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Literature summary for 1.1.1.378 extracted from

  • Watanabe, S.; Makino, K.
    Novel modified version of nonphosphorylated sugar metabolism-an alternative L-rhamnose pathway of Sphingomonas sp (2009), FEBS J., 276, 1554-1567.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26500
-
x * 26500, SDS-PAGE Sphingomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-rhamnose + NAD(P)+ Sphingomonas sp. the enzyme is part of an alternative pathway of L-rhamnose metabolism by which L-rhamnose is converted into pyruvate and L-lactaldehyde, through reaction steps analogous to the Entner-Doudoroff pathway L-rhamnono-1,4-lactone + NAD(P)H + H+
-
?
L-rhamnose + NAD(P)+ Sphingomonas sp. NBRC 101715 the enzyme is part of an alternative pathway of L-rhamnose metabolism by which L-rhamnose is converted into pyruvate and L-lactaldehyde, through reaction steps analogous to the Entner-Doudoroff pathway L-rhamnono-1,4-lactone + NAD(P)H + H+
-
?

Organism

Organism UniProt Comment Textmining
Sphingomonas sp.
-
-
-
Sphingomonas sp. NBRC 101715
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sphingomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.62
-
25°C, pH not specified in the publication, cell-free extract, NADP+-dependent activity Sphingomonas sp.
31.7
-
25°C, pH not specified in the publication, purified enzyme, NAD+-dependent activity Sphingomonas sp.
39.8
-
25°C, pH not specified in the publication, purified enzyme, NADP+-dependent activity Sphingomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lyxose + NADP+ activity is 75% compared to the activity with L-rhamnose and NADP+ Sphingomonas sp. ?
-
?
L-lyxose + NADP+ activity is 75% compared to the activity with L-rhamnose and NADP+ Sphingomonas sp. NBRC 101715 ?
-
?
L-mannose + NADP+ activity is 8.4% compared to the activity with L-rhamnose and NADP+ Sphingomonas sp. ?
-
?
L-mannose + NADP+ activity is 8.4% compared to the activity with L-rhamnose and NADP+ Sphingomonas sp. NBRC 101715 ?
-
?
L-rhamnose + NAD(P)+ the enzyme is part of an alternative pathway of L-rhamnose metabolism by which L-rhamnose is converted into pyruvate and L-lactaldehyde, through reaction steps analogous to the Entner-Doudoroff pathway Sphingomonas sp. L-rhamnono-1,4-lactone + NAD(P)H + H+
-
?
L-rhamnose + NAD(P)+ the enzyme is part of an alternative pathway of L-rhamnose metabolism by which L-rhamnose is converted into pyruvate and L-lactaldehyde, through reaction steps analogous to the Entner-Doudoroff pathway Sphingomonas sp. NBRC 101715 L-rhamnono-1,4-lactone + NAD(P)H + H+
-
?
L-rhamnose + NAD+
-
Sphingomonas sp. L-rhamnono-1,4-lactone + NADH + H+
-
?
L-rhamnose + NAD+
-
Sphingomonas sp. NBRC 101715 L-rhamnono-1,4-lactone + NADH + H+
-
?
L-rhamnose + NADP+
-
Sphingomonas sp. L-rhamnono-1,4-lactone + NADPH + H+
-
?

Subunits

Subunits Comment Organism
? x * 26500, SDS-PAGE Sphingomonas sp.

Synonyms

Synonyms Comment Organism
EAT09360
-
Sphingomonas sp.
LRA1
-
Sphingomonas sp.
NAD(P)+-dependent L-rhamnose-1-dehydrogenase
-
Sphingomonas sp.
SpLRA1
-
Sphingomonas sp.

Cofactor

Cofactor Comment Organism Structure
NAD+ dual coenzyme specificity between NAD+ and NADP+ Sphingomonas sp.
NADP+ dual coenzyme specificity between NAD+ and NADP+ Sphingomonas sp.