Cloned (Comment) | Organism |
---|---|
the recombinant enzyme is produced in Sf9 cells using a baculovirus expression system | Ips pini |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.58 | - |
(4R)-ipsdienol | pH 7.8, 25°C | Ips pini |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Ips pini | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
calculated from sequence | Ips pini |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(4R)-ipsdienol + NAD(P)+ | Ips pini | the enzyme is involved in pheromon production | ipsdienone + NAD(P)H + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ips pini | G5DGA8 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
midgut | transcript levels are highest in pheromone producing tissue: the anterior midgut of fed males. The protein is detected only in male midguts | Ips pini | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0163 | - |
pH 7.8, 25°C | Ips pini |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(4R)-ipsdienol + NAD(P)+ | the enzyme is involved in pheromon production | Ips pini | ipsdienone + NAD(P)H + H+ | - |
? | |
(4R)-ipsdienol + NAD+ | no activity with (4S)-ipsdienol. The enzyme shows similar specific activities with NAD+ and NADP+ | Ips pini | ipsdienone + NADH + H+ | - |
? | |
(4R)-ipsdienol + NADP+ | no activity with (4S)-ipsdienol. The enzyme shows similar specific activities with NAD+ and NADP+ | Ips pini | ipsdienone + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IDOLDH | - |
Ips pini |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Ips pini |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Ips pini |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | the enzyme shows similar specific activities with NAD+ and NADP+ | Ips pini | |
NADP+ | the enzyme shows similar specific activities with NAD+ and NADP+ | Ips pini |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Ips pini | calculated from sequence | - |
5.8 |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in pheromon production | Ips pini |