Cloned (Comment) | Organism |
---|---|
recombinant class I ADH g2g2 isozymes cloning and overexpression in Escherichia coli | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0153 | - |
3beta,7alpha-dihydroxy-5beta-cholan-24-oic acid | pH 7.5, 25°C, recombinant enzyme | Homo sapiens | |
0.0183 | - |
3beta-hydroxy-5beta-cholan-24-oic acid | pH 7.5, 25°C, recombinant enzyme | Homo sapiens | |
0.0238 | - |
3beta,7beta-dihydroxy-5beta-cholan-24-oic acid | pH 7.5, 25°C, recombinant enzyme | Homo sapiens | |
0.0283 | - |
3beta-hydroxy-5beta-cholan-24-oic acid | pH 7.5, 25°C, recombinant enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3beta,7beta-dihydroxy-5beta-cholan-24-oic acid + NAD+ | Homo sapiens | substrate docking of isoursodeoxycholic acid to the active site of a three-dimensional model of human class I gammagamma alcohol dehydrogenase reveals that the ADH gammagamma isozyme is the iso bile acid 3beta-hydroxysteroid dehydrogenase present in human liver cytosol, with NAD+ as a cofactor, but ethanol is the preferred substrate | 7beta-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant class I ADH g2g2 isozymes from Escherichia coli by anion exchange and 5'-AMP-affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3beta,7alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+ | i.e. isochenodeoxycholic acid | Homo sapiens | 7alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+ | - |
? | |
3beta,7beta-dihydroxy-5beta-cholan-24-oic acid + NAD+ | substrate docking of isoursodeoxycholic acid to the active site of a three-dimensional model of human class I gammagamma alcohol dehydrogenase reveals that the ADH gammagamma isozyme is the iso bile acid 3beta-hydroxysteroid dehydrogenase present in human liver cytosol, with NAD+ as a cofactor, but ethanol is the preferred substrate | Homo sapiens | 7beta-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+ | - |
? | |
3beta,7beta-dihydroxy-5beta-cholan-24-oic acid + NAD+ | i.e. isoursodeoxycholic acid | Homo sapiens | 7beta-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+ | - |
? | |
3beta-hydroxy-5beta-cholan-24-oic acid + NAD+ | i.e. isolithocholic acid | Homo sapiens | 3-oxo-5beta-cholan-24-oic acid + NADH + H+ | - |
? | |
3beta12alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+ | i.e. isodeoxycholic acid | Homo sapiens | 12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+ | - |
? | |
additional information | substrate specificity, bile acid screening, reaction product identification and quantification by gas chromotography/mass spectrometry, overview. No activity with 3alpha,7alpha,12alpha,27-tetrahydroxy-5beta-cholestane | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3beta-HSD | - |
Homo sapiens |
3beta-hydroxysteroid dehydrogenase | - |
Homo sapiens |
bile acid 3beta-HSD | - |
Homo sapiens |
iso bile acid dehydrogenase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
3beta-hydroxy-5beta-cholan-24-oic acid | pH 7.5, 25°C, recombinant enzyme | Homo sapiens | |
0.022 | - |
3beta,7alpha-dihydroxy-5beta-cholan-24-oic acid | pH 7.5, 25°C, recombinant enzyme | Homo sapiens | |
0.033 | - |
3beta,7beta-dihydroxy-5beta-cholan-24-oic acid | pH 7.5, 25°C, recombinant enzyme | Homo sapiens | |
0.047 | - |
3beta-hydroxy-5beta-cholan-24-oic acid | pH 7.5, 25°C, recombinant enzyme | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADP+ | Homo sapiens | |
NAD+ | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | epimerization of the 3beta-hydroxy to a 3alpha-hydroxy group of epi-bile acids catalyzed by 3beta-hydroxysteroid dehydrogenases and 3-oxo-reductases with 3-dehydro (oxo) bile acids as intermediate products | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.82 | - |
3beta-hydroxy-5beta-cholan-24-oic acid | pH 7.5, 25°C, recombinant enzyme | Homo sapiens | |
1.4 | - |
3beta,7alpha-dihydroxy-5beta-cholan-24-oic acid | pH 7.5, 25°C, recombinant enzyme | Homo sapiens | |
1.4 | - |
3beta,7beta-dihydroxy-5beta-cholan-24-oic acid | pH 7.5, 25°C, recombinant enzyme | Homo sapiens | |
1.66 | - |
3beta-hydroxy-5beta-cholan-24-oic acid | pH 7.5, 25°C, recombinant enzyme | Homo sapiens |