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Literature summary for 1.1.1.391 extracted from

  • Marschall, H.U.; Oppermann, U.C.; Svensson, S.; Nordling, E.; Persson, B.; Hoeoeg, J.O.; Joernvall, H.
    Human liver class I alcohol dehydrogenase gammagamma isozyme: the sole cytosolic 3beta-hydroxysteroid dehydrogenase of iso bile acids (2000), Hepatology, 31, 990-996.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
recombinant class I ADH g2g2 isozymes cloning and overexpression in Escherichia coli Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0153
-
3beta,7alpha-dihydroxy-5beta-cholan-24-oic acid pH 7.5, 25°C, recombinant enzyme Homo sapiens
0.0183
-
3beta-hydroxy-5beta-cholan-24-oic acid pH 7.5, 25°C, recombinant enzyme Homo sapiens
0.0238
-
3beta,7beta-dihydroxy-5beta-cholan-24-oic acid pH 7.5, 25°C, recombinant enzyme Homo sapiens
0.0283
-
3beta-hydroxy-5beta-cholan-24-oic acid pH 7.5, 25°C, recombinant enzyme Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3beta,7beta-dihydroxy-5beta-cholan-24-oic acid + NAD+ Homo sapiens substrate docking of isoursodeoxycholic acid to the active site of a three-dimensional model of human class I gammagamma alcohol dehydrogenase reveals that the ADH gammagamma isozyme is the iso bile acid 3beta-hydroxysteroid dehydrogenase present in human liver cytosol, with NAD+ as a cofactor, but ethanol is the preferred substrate 7beta-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant class I ADH g2g2 isozymes from Escherichia coli by anion exchange and 5'-AMP-affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3beta,7alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+ i.e. isochenodeoxycholic acid Homo sapiens 7alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
?
3beta,7beta-dihydroxy-5beta-cholan-24-oic acid + NAD+ substrate docking of isoursodeoxycholic acid to the active site of a three-dimensional model of human class I gammagamma alcohol dehydrogenase reveals that the ADH gammagamma isozyme is the iso bile acid 3beta-hydroxysteroid dehydrogenase present in human liver cytosol, with NAD+ as a cofactor, but ethanol is the preferred substrate Homo sapiens 7beta-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
?
3beta,7beta-dihydroxy-5beta-cholan-24-oic acid + NAD+ i.e. isoursodeoxycholic acid Homo sapiens 7beta-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
?
3beta-hydroxy-5beta-cholan-24-oic acid + NAD+ i.e. isolithocholic acid Homo sapiens 3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
?
3beta12alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+ i.e. isodeoxycholic acid Homo sapiens 12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
?
additional information substrate specificity, bile acid screening, reaction product identification and quantification by gas chromotography/mass spectrometry, overview. No activity with 3alpha,7alpha,12alpha,27-tetrahydroxy-5beta-cholestane Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
3beta-HSD
-
Homo sapiens
3beta-hydroxysteroid dehydrogenase
-
Homo sapiens
bile acid 3beta-HSD
-
Homo sapiens
iso bile acid dehydrogenase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.015
-
3beta-hydroxy-5beta-cholan-24-oic acid pH 7.5, 25°C, recombinant enzyme Homo sapiens
0.022
-
3beta,7alpha-dihydroxy-5beta-cholan-24-oic acid pH 7.5, 25°C, recombinant enzyme Homo sapiens
0.033
-
3beta,7beta-dihydroxy-5beta-cholan-24-oic acid pH 7.5, 25°C, recombinant enzyme Homo sapiens
0.047
-
3beta-hydroxy-5beta-cholan-24-oic acid pH 7.5, 25°C, recombinant enzyme Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADP+ Homo sapiens
NAD+
-
Homo sapiens

General Information

General Information Comment Organism
metabolism epimerization of the 3beta-hydroxy to a 3alpha-hydroxy group of epi-bile acids catalyzed by 3beta-hydroxysteroid dehydrogenases and 3-oxo-reductases with 3-dehydro (oxo) bile acids as intermediate products Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.82
-
3beta-hydroxy-5beta-cholan-24-oic acid pH 7.5, 25°C, recombinant enzyme Homo sapiens
1.4
-
3beta,7alpha-dihydroxy-5beta-cholan-24-oic acid pH 7.5, 25°C, recombinant enzyme Homo sapiens
1.4
-
3beta,7beta-dihydroxy-5beta-cholan-24-oic acid pH 7.5, 25°C, recombinant enzyme Homo sapiens
1.66
-
3beta-hydroxy-5beta-cholan-24-oic acid pH 7.5, 25°C, recombinant enzyme Homo sapiens