KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.4 | - |
acetoin | cosubstrate NADH, pH 7.0, temperature not specified in the publication | Escherichia coli | |
13.6 | - |
acetoin | cosubstrate NADPH, pH 7.0, temperature not specified in the publication | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli BW25113 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetoin + NADH + H+ | - |
Escherichia coli | (R,R)-butanediol + NAD+ | - |
? | |
acetoin + NADH + H+ | - |
Escherichia coli BW25113 | (R,R)-butanediol + NAD+ | - |
? | |
acetoin + NADPH + H+ | - |
Escherichia coli | (R,R)-butanediol + NADP+ | - |
? | |
acetoin + NADPH + H+ | - |
Escherichia coli BW25113 | (R,R)-butanediol + NADP+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
acetoin | cosubstrate NADPH, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.4 | - |
acetoin | cosubstrate NADH, pH 7.0, temperature not specified in the publication | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | preferred over NADPH | Escherichia coli | |
NADPH | NADH is preferred over NADPH | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | glycerol/1,2-propanediol dehydrogenase GldA is the major enzyme responsible for the acetoin reducing activity observed in Escherichia coli | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
acetoin | cosubstrate NADPH, pH 7.0, temperature not specified in the publication | Escherichia coli | |
0.4 | - |
acetoin | cosubstrate NADH, pH 7.0, temperature not specified in the publication | Escherichia coli |