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Literature summary for 1.1.1.4 extracted from

  • Liang, K.; Shen, C.R.
    Selection of an endogenous 2,3-butanediol pathway in Escherichia coli by fermentative redox balance (2017), Metab. Eng., 39, 181-191.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4
-
acetoin cosubstrate NADH, pH 7.0, temperature not specified in the publication Escherichia coli
13.6
-
acetoin cosubstrate NADPH, pH 7.0, temperature not specified in the publication Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli BW25113
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetoin + NADH + H+
-
Escherichia coli (R,R)-butanediol + NAD+
-
?
acetoin + NADH + H+
-
Escherichia coli BW25113 (R,R)-butanediol + NAD+
-
?
acetoin + NADPH + H+
-
Escherichia coli (R,R)-butanediol + NADP+
-
?
acetoin + NADPH + H+
-
Escherichia coli BW25113 (R,R)-butanediol + NADP+
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.5
-
acetoin cosubstrate NADPH, pH 7.0, temperature not specified in the publication Escherichia coli
1.4
-
acetoin cosubstrate NADH, pH 7.0, temperature not specified in the publication Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NADH preferred over NADPH Escherichia coli
NADPH NADH is preferred over NADPH Escherichia coli

General Information

General Information Comment Organism
physiological function glycerol/1,2-propanediol dehydrogenase GldA is the major enzyme responsible for the acetoin reducing activity observed in Escherichia coli Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.04
-
acetoin cosubstrate NADPH, pH 7.0, temperature not specified in the publication Escherichia coli
0.4
-
acetoin cosubstrate NADH, pH 7.0, temperature not specified in the publication Escherichia coli