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Literature summary for 1.1.1.40 extracted from

  • Wheeler, M.C.; Tronconi, M.A.; Drincovich, M.F.; Andreo, C.S.; Fluegge, U.I.; Maurino, V.G.
    A comprehensive analysis of the NADP-malic enzyme gene family of Arabidopsis (2005), Plant Physiol., 139, 39-51.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.40

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, NADP-ME1 Arabidopsis thaliana
expression in Escherichia coli, NADP-ME2 Arabidopsis thaliana
expression in Escherichia coli, NADP-ME3 Arabidopsis thaliana
expression in Escherichia coli, NADP-ME4 Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00296
-
 (S)-malate pH 7.5, NADP-ME1 Arabidopsis thaliana
0.0033
-
 (S)-malate pH 7.5, NADP-ME2 Arabidopsis thaliana
0.0065
-
 NADP+ pH 7.5, NADP-ME3 Arabidopsis thaliana
0.0102
-
 NADP+ pH 7.5, NADP-ME4 Arabidopsis thaliana
0.0721
-
 NADP+ pH 7.5, NADP-ME2 Arabidopsis thaliana
0.205
-
 NADP+ pH 7.5, NADP-ME1 Arabidopsis thaliana
0.23
-
 (S)-malate pH 7.5, NADP-ME4 Arabidopsis thaliana
0.83
-
 (S)-malate pH 7.5, NADP-ME3 Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
plastid NADP-ME4 is localized to plastids Arabidopsis thaliana 9536
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon NADP-ME4 is found in guard cells of cotyledonous leaves Arabidopsis thaliana
-
embryo activity is detected in the central vasculature of the siliques Arabidopsis thaliana
-
embryo activity is detected in the embryo at all developmental stages, including the seed attachment point and the integuments, NADP-ME4 Arabidopsis thaliana
-
embryo at the globular and heart stage, detected in the funiculus and vascular tissue of the siliques, NADP-ME2 Arabidopsis thaliana
-
embryo expression of NADP-ME1 from the torpedo stage onward Arabidopsis thaliana
-
endosperm NADP-ME4 Arabidopsis thaliana
-
filament NADP-ME4 Arabidopsis thaliana
-
flower sepals and filaments of developed flowers, NADP-ME2 Arabidopsis thaliana
-
hypocotyl NADP-ME4 Arabidopsis thaliana
-
leaf expression of NADP-ME2 in all cell types, being particularly strong in the trichome basal cells and hydatodes Arabidopsis thaliana
-
leaf expression of NADP-ME3 is restricted to the trichomes and trichome basal cells of leaves and stems Arabidopsis thaliana
-
leaf NADP-ME4 Arabidopsis thaliana
-
additional information NADP-ME2 activity is detected in all reproductive organs Arabidopsis thaliana
-
additional information NADP-ME4 is detected in all vegetative organs Arabidopsis thaliana
-
additional information no activity of NADP-ME1 is detectable in leaves and stems throughout growth, not expressed in floral organs Arabidopsis thaliana
-
petiole NADP-ME4 Arabidopsis thaliana
-
pollen expression of NADP-ME3 exclusively in pollen from the latest maturation stage up to its germination on the stigma Arabidopsis thaliana
-
pollen NADP-ME4 Arabidopsis thaliana
-
root expression of NADP-ME2 throughout all the tissues, except for root tips Arabidopsis thaliana
-
root stele, mainly at emerging lateral roots and at root tips, NADP-ME4 Arabidopsis thaliana
-
secondary root NADP-ME1 expression only in some secondary roots, where it is confined to the stele and excluded from the tips Arabidopsis thaliana
-
sepal NADP-ME2 Arabidopsis thaliana
-
sepal NADP-ME4 Arabidopsis thaliana
-
silique in developing siliques, NADP-ME2 activity is found at both ends, the stigmatic papillae and the abscission zone. Detected in the funiculus and vascular tissue of the siliques, NADP-ME2 Arabidopsis thaliana
-
stele of root Arabidopsis thaliana
-
stem expression of NADP-ME3 is restricted to the trichomes and trichome basal cells of leaves and stems Arabidopsis thaliana
-
stem NADP-ME4 Arabidopsis thaliana
-
stigma NADP-ME4 Arabidopsis thaliana
-
vascular system NADP-ME4 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NADP+
-
 Arabidopsis thaliana pyruvate + NADPH + CO2
-
 ?

Synonyms

Synonyms Comment Organism
NADP-ME1
-
 Arabidopsis thaliana
NADP-ME2
-
 Arabidopsis thaliana
NADP-ME3
-
 Arabidopsis thaliana
NADP-ME4
-
 Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
38.7
-
 NADP+ pH 7.5, NADP-ME1 Arabidopsis thaliana
151.3
-
 NADP+ pH 7.5, NADP-ME4 Arabidopsis thaliana
268.1
-
 NADP+ pH 7.5, NADP-ME3 Arabidopsis thaliana
324.1
-
 NADP+ pH 7.5, NADP-ME2 Arabidopsis thaliana
4670
-
 NADP+ pH 7.5, NADP-ME2 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Arabidopsis thaliana