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Literature summary for 1.1.1.40 extracted from
- Nicotiana tabacum NADP-malic enzyme: cloning, characterization and analysis of biological role (2008), Plant Cell Physiol., 49, 469-480.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| aspartate | 2fold activation of isozyme NADP-ME1 | Nicotiana tabacum |  |
| CoA | slight activation of isozyme NADP-ME2 | Nicotiana tabacum | |
| additional information | no activation of isozyme NADP-ME2 by aspartate | Nicotiana tabacum |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| isozyme NADP-ME2, expression in Escherichia coli | Nicotiana tabacum |
| nadp-me1, expression in Escherichia coli | Nicotiana tabacum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | 40% inhibition of isozyme NADP-ME1 at 2 mM; inhibition of isozyme NADP-ME2 | Nicotiana tabacum | |
| ATP | 20% inhibition of isozyme NADP-ME1 at 2 mM; inhibition of isozyme NADP-ME2 | Nicotiana tabacum | |
| CoA | 30% inhibition of isozyme NADP-ME1 at 2 mM | Nicotiana tabacum | |
| D-glucose 6-phosphate | 40% inhibition of isozyme NADP-ME1 at 2 mM; inhibition of isozyme NADP-ME2 | Nicotiana tabacum | |
| fumarate | 20% inhibition of isozyme NADP-ME1 at 2 mM; inhibition of isozyme NADP-ME2 | Nicotiana tabacum | |
| malate | inhibition of isozyme NADP-ME1 at pH 7.0 | Nicotiana tabacum | |
| additional information | no inhibition of isozyme NADP-ME2 by aspartate and malate | Nicotiana tabacum | |
| oxaloacetic acid | 60% inhibition of isozyme NADP-ME1 at 2 mM; inhibition of isozyme NADP-ME2 | Nicotiana tabacum | |
| pyruvate | 20% inhibition of isozyme NADP-ME1 at 2 mM; inhibition of isozyme NADP-ME2 | Nicotiana tabacum | |
| succinate | inhibition of isozyme NADP-ME2 | Nicotiana tabacum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00171 | - |
NADP+ | pH 7.3, isozyme NADP-ME1 | Nicotiana tabacum | |
| 0.00429 | - |
NADP+ | pH 7.0, isozyme NADP-ME2 | Nicotiana tabacum | |
| 0.13 | - |
(S)-malate | pH 7.3, isozyme NADP-ME1 | Nicotiana tabacum | |
| 0.69 | - |
pyruvate | pH 7.0, isozyme NADP-ME1 | Nicotiana tabacum | |
| 1.46 | - |
(S)-malate | pH 7.0, isozyme NADP-ME2 | Nicotiana tabacum | |
| 2.6 | - |
pyruvate | pH 7.0, isozyme NADP-ME2 | Nicotiana tabacum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Nicotiana tabacum | 9507 | - |
| cytosol | - |
Nicotiana tabacum | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 63000 | - |
4 * 63000, plastidic isozyme, SDS-PAGE | Nicotiana tabacum |
| 65000 | - |
4 * 65000, cytosolic isozyme, SDS-PAGE | Nicotiana tabacum |
| 260000 | - |
native PAGE | Nicotiana tabacum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NADP+ | Nicotiana tabacum | - |
pyruvate + CO2 + NADPH | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Nicotiana tabacum | Q006P9 | gene nadp-me2; gene nadp-me2 | - |
| Nicotiana tabacum | Q006Q0 | gene nadp-me1; gene nadp-me1 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | - |
Nicotiana tabacum | - |
| leaf | - |
Nicotiana tabacum | - |
| root | - |
Nicotiana tabacum | - |
| stem | - |
Nicotiana tabacum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.048 | - |
enzyme extracts from flowers | Nicotiana tabacum |
| 0.069 | - |
enzyme extracts from stems | Nicotiana tabacum |
| 0.072 | - |
enzyme extracts from leaves | Nicotiana tabacum |
| 0.571 | - |
enzyme extract from roots | Nicotiana tabacum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NADP+ | - |
Nicotiana tabacum | pyruvate + CO2 + NADPH | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 63000, plastidic isozyme, SDS-PAGE | Nicotiana tabacum |
| tetramer | 4 * 65000, cytosolic isozyme, SDS-PAGE | Nicotiana tabacum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| L-malate:NADP oxidoreductase (oxaloacetate decarboxylating) | - |
Nicotiana tabacum |
| NADP-malic enzyme | - |
Nicotiana tabacum |
| NADP-ME | - |
Nicotiana tabacum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 21.6 | - |
pyruvate | pH 7.0, isozyme NADP-ME2 | Nicotiana tabacum | |
| 154.3 | - |
(S)-malate | pH 7.3, isozyme NADP-ME1 | Nicotiana tabacum | |
| 158.4 | - |
pyruvate | pH 7.0, isozyme NADP-ME1 | Nicotiana tabacum | |
| 177 | - |
(S)-malate | pH 7.0, isozyme NADP-ME2 | Nicotiana tabacum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
isozyme NADP-ME1, reverse reaction | Nicotiana tabacum |
| 7 | - |
isozyme NADP-ME2, forward and reverse reaction | Nicotiana tabacum |
| 7.3 | - |
isozyme NADP-ME1, forward reaction | Nicotiana tabacum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Nicotiana tabacum | |
| NADPH | - |
Nicotiana tabacum | |
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