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Literature summary for 1.1.1.40 extracted from

  • Rodriguez, E.; Navone, L.; Casati, P.; Gramajo, H.
    Impact of malic enzymes on antibiotic and triacylglycerol production in Streptomyces coelicolor (2012), Appl. Environ. Microbiol., 78, 4571-4579.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene Sco5261, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expressionin Escherichia coli strain BL21(DE3) Streptomyces coelicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.107
-
NADP+ pH 8.0, 30°C, recombinant enzyme Streptomyces coelicolor
6.4
-
(S)-malate pH 8.0, 30°C, recombinant enzyme Streptomyces coelicolor
18.1
-
pyruvate pH 8.0, 30°C, recombinant enzyme Streptomyces coelicolor

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NADP+ Streptomyces coelicolor
-
pyruvate + CO2 + NADPH
-
r
(S)-malate + NADP+ Streptomyces coelicolor M145
-
pyruvate + CO2 + NADPH
-
r

Organism

Organism UniProt Comment Textmining
Streptomyces coelicolor Q9F3K4 gene Sco5261
-
Streptomyces coelicolor M145 Q9F3K4 gene Sco5261
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NADP+
-
Streptomyces coelicolor pyruvate + CO2 + NADPH
-
r
(S)-malate + NADP+
-
Streptomyces coelicolor M145 pyruvate + CO2 + NADPH
-
r

Synonyms

Synonyms Comment Organism
malic enzyme-NADP
-
Streptomyces coelicolor
ME-NADP
-
Streptomyces coelicolor

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Streptomyces coelicolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Streptomyces coelicolor

Cofactor

Cofactor Comment Organism Structure
NADP+ dependent on Streptomyces coelicolor
NADPH
-
Streptomyces coelicolor

General Information

General Information Comment Organism
malfunction the double Sco2951 Sco5261 mutant, deficient in ME-NAD, EC 1.1.1.39, and ME-NADP activity, display a strong reduction in the production of the polyketide antibiotic actinorhodin. Additionally, the Sco2951/Sco5261 mutant shows a decrease in stored triacylglcerides during exponential growth Streptomyces coelicolor
physiological function the enzyme plays a role in antibiotic and triacylglycerol production, e.g. production of the polyketide antibiotic actinorhodin, overview Streptomyces coelicolor