Any feedback?
Literature summary for 1.1.1.40 extracted from
- Fumarate and cytosolic pH as modulators of the synthesis or consumption of C4 organic acids through NADP-malic enzyme in Arabidopsis thaliana (2013), Plant Mol. Biol., 81, 297-307.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| fumarate | fumarate activates the NADP-ME2 forward reaction by about 15fold at 0.05 mM NADP+, but only at about 1.5fold at 0.35 mM NADP+. The trans configuration of fumarate is crucial for the activating effect | Arabidopsis thaliana |  |
| succinate | activates at 10 mM | Arabidopsis thaliana | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of isozyme NADP-ME2 in Escherichia coli strain BL21(DE3) | Arabidopsis thaliana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| R115A | site-directed mutagenesis of isozyme NADP-ME2, the mutant displays a marked inhibition in the presence of all the organic acids tested, also fumarate | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxoglutarate | inhibition of isozyme NADP-ME2 | Arabidopsis thaliana | |
| Maleate | the cis isomer of fumarate, inhibition of isozyme NADP-ME2 | Arabidopsis thaliana | |
| malonate | inhibition of isozyme NADP-ME2 | Arabidopsis thaliana | |
| additional information | no inhibition of isozyme NADP-ME2 by tartrate | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics of NADP+ and (S)-malate at different NADP+ and fumarate concentrations, overview | Arabidopsis thaliana |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Arabidopsis thaliana | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NADP+ | Arabidopsis thaliana | - |
pyruvate + CO2 + NADPH | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
four malic isozymes, NADP-ME1, NADP-ME2, NADP-ME3, and NADP-ME4 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NADP+ | - |
Arabidopsis thaliana | pyruvate + CO2 + NADPH | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NADP-dependent malic enzyme | - |
Arabidopsis thaliana |
| NADP-ME | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
reductive carboxylation of pyruvate | Arabidopsis thaliana |
| 7.5 | - |
oxidative decarboxylation of malate | Arabidopsis thaliana |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | dependent on | Arabidopsis thaliana | |
| NADPH | dependent on | Arabidopsis thaliana | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
