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Literature summary for 1.1.1.404 extracted from

  • Chen, L.; Yang, J.
    Biochemical characterization of the tetrachlorobenzoquinone reductase involved in the biodegradation of pentachlorophenol (2008), Int. J. Mol. Sci., 9, 198-212.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.404

Activating Compound

Activating Compound Comment Organism Structure
tetrachlorohydroquinone activity of PcpD is stimulated by tetrachlorohydroquinone at low concentrations but inhibited at high concentrations. 83% increase in activity at 0.1 mM tetrachlorohydroquinone, 60% loss of activity at 0.2 mM tetrachlorohydroquinone Sphingobium chlorophenolicum

Cloned(Commentary)

Cloned (Comment) Organism
expression in Eshcerichia coli Sphingobium chlorophenolicum

Crystallization (Commentary)

Crystallization (Comment) Organism
homology modeling of structure. The active site is slightly positively charged and situated in a deep pit on the surface. The putative binding pocket is adjacent to the cofactor flavin mononucleotide and the 2Fe-2S cluster. It is formed mainly by residues Ser78, Arg79, Phe225, Gly226, Ala227, Ala228, Leu229, Gln275 and the cofactor FMN Sphingobium chlorophenolicum

Inhibitors

Inhibitors Comment Organism Structure
Pentachlorophenol competitive, 55% loss of activity at 0.1 mM Sphingobium chlorophenolicum
tetrachlorohydroquinone activity of PcpD is stimulated by tetrachlorohydroquinone at low concentrations but inhibited at high concentrations. 83% increase in activity at 0.1 mM tetrachlorohydroquinone, 60% loss of activity at 0.2 mM tetrachlorohydroquinone Sphingobium chlorophenolicum

Metals/Ions

Metals/Ions Comment Organism Structure
Iron protein contains 1.7 mol of iron per mol Sphingobium chlorophenolicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 protein exists as trimer, dynamic light scattering Sphingobium chlorophenolicum
36000
-
-
 Sphingobium chlorophenolicum
36500
-
-
 Sphingobium chlorophenolicum

Organism

Organism UniProt Comment Textmining
Sphingobium chlorophenolicum Q47914
-
-
Sphingobium chlorophenolicum ATCC 39723 Q47914
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein,. Purified enzyme is colorless Sphingobium chlorophenolicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1
-
 pH 7.0, 25°C Sphingobium chlorophenolicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3,5,6-tetrachlorohydroquinone + NAD+
-
 Sphingobium chlorophenolicum 2,3,5,6-tetrachloro-1,4-benzoquinone + NADH + H+
-
 ?
2,3,5,6-tetrachlorohydroquinone + NAD+
-
 Sphingobium chlorophenolicum ATCC 39723 2,3,5,6-tetrachloro-1,4-benzoquinone + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
homotrimer 3 * 36000, SDS-PAGE, 3 * 36500, mass spectrometry, 3 * 36500, calculated Sphingobium chlorophenolicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Sphingobium chlorophenolicum

pH Range

pH Minimum pH Maximum Comment Organism
5
-
 70% of maximum activity Sphingobium chlorophenolicum
8
-
 20% of maximum activity Sphingobium chlorophenolicum

Cofactor

Cofactor Comment Organism Structure
FMN the putative binding pocket is adjacent to the cofactor flavin mononucleotide and the 2Fe-2S cluster Sphingobium chlorophenolicum
[2Fe-2S]-center the putative binding pocket is adjacent to the cofactor flavin mononucleotide and the 2Fe-2S cluster Sphingobium chlorophenolicum