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Literature summary for 1.1.1.405 extracted from

  • Follens, A.; Veiga-da-Cunha, M.; Merckx, R.; van Schaftingen, E.; van Eldere, J.
    acs1 of Haemophilus influenzae type a capsulation locus region II encodes a bifunctional ribulose 5-phosphate reductase- CDP-ribitol pyrophosphorylase (1999), J. Bacteriol., 181, 2001-2007.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
CTP marked stimulation, presence of 50 mM CTP decreases the Km value for ribulose 5-phosphate from 400 to 50 microM Haemophilus influenzae
additional information not stimulated by UTP, ATP, GTP, ADP, or dCTP Haemophilus influenzae

Cloned(Commentary)

Cloned (Comment) Organism
expression in ERscherichia coli Haemophilus influenzae

General Stability

General Stability Organism
23°C, at 0.3 mg/ml in the presence of 20 mM HEPES, pH 7.1, 1 mM DTT, and 0.5 mg of BSA/ml, ribitol 5-phosphate dehydrogenase activity decreases to about 50% of the initial activity after 2 h Haemophilus influenzae

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibitory: 0.5 mM ribitol 5-phosphate or 0.5 mM NADP+ Haemophilus influenzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
NADPH pH 7.1, 30°C Haemophilus influenzae
0.05
-
D-ribitol 5-phosphate presence of CTP, pH 7.1, 30°C Haemophilus influenzae
0.4
-
D-ribitol 5-phosphate pH 7.1, 30°C Haemophilus influenzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
x * 53000, SDS-PAGE Haemophilus influenzae

Organism

Organism UniProt Comment Textmining
Haemophilus influenzae Q48154 additionally catalyzes reaction of ribitol-5-phosphate cytidylyltransferase, EC 2.7.7.40
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.73
-
substrate D-ribulose 5-phosphate, pH 8.5, 30°C Haemophilus influenzae
2.87
-
substrate D-ribulose 5-phosphate, presence of CTP, pH 8.5, 30°C Haemophilus influenzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribitol 5-phosphate + NADP+
-
Haemophilus influenzae D-ribulose 5-phosphate + NADPH + H+
-
r
D-ribulose 5-phosphate + NADPH + H+
-
Haemophilus influenzae D-ribitol 5-phosphate + NADP+
-
r
additional information the dehydrogenase activity of Acs1 is specific for ribulose 5-phosphate and NADPH in one direction and for ribitol 5-phosphate and NADP+ in the other direction. The activity is about twofold higher with ribulose 5-phosphate as the substrate than with ribose 5-phosphate. No substrate: xylulose 5-phosphate Haemophilus influenzae ?
-
?

Subunits

Subunits Comment Organism
? x * 53000, SDS-PAGE Haemophilus influenzae

Synonyms

Synonyms Comment Organism
ACS1
-
Haemophilus influenzae
bifunctional ribulose 5-phosphate reductase/CDP-ribitol pyrophosphorylase
-
Haemophilus influenzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8.4 broad Haemophilus influenzae

General Information

General Information Comment Organism
physiological function Acs1 encodes a bifunctional enzyme that converts ribulose 5-phosphate into ribitol 5-phosphate and further into CDP-ribitol, the activated precursor form for incorporation of ribitol 5-phosphate into the Haemophilus influenzae type a capsular polysaccharide Haemophilus influenzae