Cloned (Comment) | Organism |
---|---|
construction of a genomic cDNA library, gene Cl-idh, DNA sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) | Chlorobium limicola |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition of carboxylation by citrate, pyruvate, succinate, fumarate, malate, glyoxylate, ATP, and ADP | Chlorobium limicola | |
oxaloacetate | competitive, inhibits the reverse reaction, over 50% inhibition at 1 mM, synergistically with glyoxylate | Chlorobium limicola |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
NADP+ | recombinant enyme, pH 9.0 | Chlorobium limicola | |
0.045 | - |
isocitrate | recombinant enyme, pH 9.0 | Chlorobium limicola | |
1.1 | - |
2-oxoglutarate | recombinant enyme, pH 7.0 | Chlorobium limicola | |
1.3 | - |
CO2 | recombinant enyme, pH 7.0 | Chlorobium limicola |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
native PAGE | Chlorobium limicola |
81000 | - |
gel filtration | Chlorobium limicola |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | Chlorobium limicola | carbon dioxide-fixing enzyme in the reductive tricarboxylic acid cycle, preference for carboxylation reaction direction | 2-oxoglutarate + CO2 + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlorobium limicola | - |
green sulfur bacterium, strain M1 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli by ion exchange and gel filtration to homogeneity, 1.84fold | Chlorobium limicola |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
36 | - |
purified recombinant enzyme | Chlorobium limicola |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | - |
Chlorobium limicola | 2-oxoglutarate + CO2 + NADPH + H+ | - |
r | |
isocitrate + NADP+ | carbon dioxide-fixing enzyme in the reductive tricarboxylic acid cycle, preference for carboxylation reaction direction | Chlorobium limicola | 2-oxoglutarate + CO2 + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 80000 | Chlorobium limicola |
Synonyms | Comment | Organism |
---|---|---|
isocitrate dehydrogenase | - |
Chlorobium limicola |
NADP-dependent IDH | - |
Chlorobium limicola |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
reverse reaction | Chlorobium limicola |
9 | - |
forward reaction | Chlorobium limicola |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADH | Chlorobium limicola | |
NADP+ | - |
Chlorobium limicola | |
NADPH | - |
Chlorobium limicola |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.35 | - |
oxaloacetate | recombinant enzyme, pH 7.0 | Chlorobium limicola |