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Literature summary for 1.1.1.42 extracted from

  • Huang, Y.C.; Colman, R.F.
    Location of the coenzyme binding site in the porcine mitochondrial NADP-dependent isocitrate dehydrogenase (2005), J. Biol. Chem., 280, 30349-30353.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with Mn2+ and isocitrate Sus scrofa

Protein Variants

Protein Variants Comment Organism
N328D 36% decrease in vmax-value compared to wild-type Sus scrofa
N328S slight decrease in vmax-value compared to wild-type Sus scrofa
R83K slight decrease in vmax-value compared to wild-type Sus scrofa
R83Q slight decrease in vmax-value compared to wild-type Sus scrofa
T311A slight decrease in vmax-value compared to wild-type Sus scrofa
T311N vmax-value is less than 1% of the value of wild-type Sus scrofa
T311S large increase in vmax-value compared to wild-type Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0043
-
NADP+ mutant T311N, pH 7.4, 25°C Sus scrofa
0.0056
-
NADP+ wild-type, pH 7.4, 25°C Sus scrofa
0.0064
-
DL-isocitrate mutant T311S, pH 7.4, 25°C Sus scrofa
0.0065
-
DL-isocitrate mutant R83K, pH 7.4, 25°C Sus scrofa
0.0073
-
DL-isocitrate mutant T311N, pH 7.4, 25°C Sus scrofa
0.0084
-
DL-isocitrate wild-type, pH 7.4, 25°C Sus scrofa
0.0097
-
NADP+ mutant T311S, pH 7.4, 25°C Sus scrofa
0.0114
-
DL-isocitrate mutant N328D, pH 7.4, 25°C Sus scrofa
0.014
-
DL-isocitrate mutant R83Q, pH 7.4, 25°C Sus scrofa
0.0154
-
DL-isocitrate mutant N328S, pH 7.4, 25°C Sus scrofa
0.0155
-
DL-isocitrate mutant T311A, pH 7.4, 25°C Sus scrofa
0.0203
-
NADP+ mutant R83K, pH 7.4, 25°C Sus scrofa
0.0246
-
NADP+ mutant N328D, pH 7.4, 25°C Sus scrofa
0.0749
-
NADP+ mutant T311A, pH 7.4, 25°C Sus scrofa
0.0811
-
NADP+ mutant R83Q, pH 7.4, 25°C Sus scrofa
0.119
-
NADP+ mutant N328S, pH 7.4, 25°C Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Sus scrofa 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ wild-type, Km-value 0.00033 mM Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa P33198
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-isocitrate + NADP+
-
Sus scrofa 2-oxoglutarate + CO2 + NADPH
-
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