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Literature summary for 1.1.1.42 extracted from
- Expression and characterization of a novel isocitrate dehydrogenase from Streptomyces diastaticus No. 7 strain M1033 (2013), Mol. Biol. Rep., 40, 1615-1623.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| SdIDH, sequence comparisons, expression of His6-tagged enzyme in Escherichia coli strain Rosetta (DE3) | Streptomyces diastaticus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Streptomyces diastaticus |  |
| Co2+ | - |
Streptomyces diastaticus | |
| Cu2+ | complete inhibition | Streptomyces diastaticus | |
| EDTA | - |
Streptomyces diastaticus | |
| K+ | - |
Streptomyces diastaticus | |
| Na+ | - |
Streptomyces diastaticus | |
| Zn2+ | - |
Streptomyces diastaticus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activates, about 50% activity compared to Mn2+, SdIDH displays a 19000-32000fold (kcat/Km) preference for NADP+ over NAD+ with Mn2+ and Mg2+, respectively | Streptomyces diastaticus | |
| Mn2+ | activates, preferred cation, SdIDH displays a 19000-32000fold (kcat/Km) preference for NADP+ over NAD+ with Mn2+ and Mg2+, respectively | Streptomyces diastaticus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
gel filtration | Streptomyces diastaticus |
| 80000 | - |
1 * 80000, SDS-PAGE | Streptomyces diastaticus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DL-isocitrate + NADP+ | Streptomyces diastaticus | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
| DL-isocitrate + NADP+ | Streptomyces diastaticus M1033 | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces diastaticus | - |
- |
- |
| Streptomyces diastaticus M1033 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme from Escherichia coli strain Rosetta (DE3) | Streptomyces diastaticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DL-isocitrate + NAD+ | low activityy with NAD+ | Streptomyces diastaticus | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
| DL-isocitrate + NAD+ | low activityy with NAD+ | Streptomyces diastaticus M1033 | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
| DL-isocitrate + NADP+ | - |
Streptomyces diastaticus | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
| DL-isocitrate + NADP+ | NADP+ is the highly preferred cofactor | Streptomyces diastaticus | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
| DL-isocitrate + NADP+ | - |
Streptomyces diastaticus M1033 | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
| DL-isocitrate + NADP+ | NADP+ is the highly preferred cofactor | Streptomyces diastaticus M1033 | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 80000, SDS-PAGE | Streptomyces diastaticus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| IDH | - |
Streptomyces diastaticus |
| NADP+-dependent IDH | - |
Streptomyces diastaticus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
with Mg2+ | Streptomyces diastaticus |
| 40 | - |
with Mn2+ | Streptomyces diastaticus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 45 | activity range, profile overview | Streptomyces diastaticus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
purified enzyme, 20 min, stable up to 40°C | Streptomyces diastaticus |
| 47 | - |
purified enzyme, 20 min, loss of 50% activity | Streptomyces diastaticus |
| 50 | - |
purified enzyme, 20 min, inactivation | Streptomyces diastaticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
with Mn2+ | Streptomyces diastaticus |
| 9 | - |
with Mg2+ | Streptomyces diastaticus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 11 | activity range, profile overview | Streptomyces diastaticus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | SdIDH displays a 19000-32000fold (kcat/Km) preference for NADP+ over NAD+ with Mn2+ and Mg2+, respectively | Streptomyces diastaticus | |
| NAD+ | poor cofactor | Streptomyces diastaticus | |
| NADP+ | dependent on | Streptomyces diastaticus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | NAD(P)+-dependent isocitrate dehydrogenase is a key enzyme in tricarboxylic acid cycle | Streptomyces diastaticus |
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