General Stability | Organism |
---|---|
denaturation by urea | Escherichia coli |
stable in 8 M urea, nearly unaffected, only 10% loss of activity after exposure for 60 min | Geobacillus stearothermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) | - |
Cyberlindnera jadinii | |
5,5'-dithiobis(2-nitrobenzoic acid) | - |
Enterococcus faecalis | |
5,5'-dithiobis(2-nitrobenzoic acid) | - |
Escherichia coli | |
5,5'-dithiobis(2-nitrobenzoic acid) | - |
Geobacillus stearothermophilus | |
D-fructose 1,6-diphosphate | - |
Enterococcus faecalis | |
D-fructose 1,6-diphosphate | - |
Escherichia coli | |
D-fructose 1,6-diphosphate | - |
Neurospora crassa | |
NADPH | - |
Escherichia coli | |
NADPH | - |
Geobacillus stearothermophilus | |
NADPH | - |
Neurospora crassa | |
p-chloromercuribenzoate | - |
Enterococcus faecalis | |
p-chloromercuribenzoate | - |
Escherichia coli | |
p-chloromercuribenzoate | - |
Geobacillus stearothermophilus | |
Rose bengal | photooxidation | Escherichia coli | |
Rose bengal | photooxidation | Geobacillus stearothermophilus | |
Rose bengal | photooxidation | Neurospora crassa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
NADP+ | - |
Geobacillus stearothermophilus | |
0.05 | - |
6-phosphogluconate | - |
Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
gel filtration | Cyberlindnera jadinii |
100000 | - |
gel filtration | Escherichia coli |
100000 | - |
sucrose density gradient centrifugation | Geobacillus stearothermophilus |
106000 | 115000 | - |
Enterococcus faecalis |
110000 | 120000 | - |
Neurospora crassa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-phospho-D-gluconate + NADP+ | Cyberlindnera jadinii | - |
D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
6-phospho-D-gluconate + NADP+ | Enterococcus faecalis | - |
D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
6-phospho-D-gluconate + NADP+ | Escherichia coli | enzyme specific for the oxidative pentose phosphate pathway | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
6-phospho-D-gluconate + NADP+ | Geobacillus stearothermophilus | enzyme specific for the oxidative pentose phosphate pathway | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
6-phospho-D-gluconate + NADP+ | Neurospora crassa | enzyme specific for the oxidative pentose phosphate pathway | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
6-phospho-D-gluconate + NADP+ | Escherichia coli MRE 600 | enzyme specific for the oxidative pentose phosphate pathway | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cyberlindnera jadinii | - |
- |
- |
Enterococcus faecalis | - |
- |
- |
Escherichia coli | - |
MRE 600 | - |
Escherichia coli MRE 600 | - |
MRE 600 | - |
Geobacillus stearothermophilus | - |
- |
- |
Neurospora crassa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
32 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
20°C, in phosphate buffer pH 6.8 in presence of the reducing agent beta-mercaptoethanol enzyme remains fully active for 24 h | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-phospho-D-gluconate + NADP+ | - |
Cyberlindnera jadinii | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
6-phospho-D-gluconate + NADP+ | - |
Escherichia coli | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
6-phospho-D-gluconate + NADP+ | - |
Geobacillus stearothermophilus | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
6-phospho-D-gluconate + NADP+ | - |
Neurospora crassa | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
6-phospho-D-gluconate + NADP+ | - |
Enterococcus faecalis | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
6-phospho-D-gluconate + NADP+ | enzyme specific for the oxidative pentose phosphate pathway | Escherichia coli | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
6-phospho-D-gluconate + NADP+ | enzyme specific for the oxidative pentose phosphate pathway | Geobacillus stearothermophilus | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
6-phospho-D-gluconate + NADP+ | enzyme specific for the oxidative pentose phosphate pathway | Neurospora crassa | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
6-phospho-D-gluconate + NADP+ | - |
Escherichia coli MRE 600 | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
6-phospho-D-gluconate + NADP+ | enzyme specific for the oxidative pentose phosphate pathway | Escherichia coli MRE 600 | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Cyberlindnera jadinii |
dimer | - |
Escherichia coli |
dimer | - |
Geobacillus stearothermophilus |
dimer | - |
Neurospora crassa |
dimer | - |
Enterococcus faecalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 60 | - |
Escherichia coli |
75 | 80 | - |
Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
stable below | Escherichia coli |
60 | - |
retains more than 90% of its structure | Geobacillus stearothermophilus |
67 | - |
stable below | Geobacillus stearothermophilus |
70 | - |
stable up to | Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Cyberlindnera jadinii | |
NADP+ | - |
Escherichia coli | |
NADP+ | - |
Neurospora crassa | |
NADP+ | - |
Enterococcus faecalis | |
NADP+ | strictly specific for NADP+, no activity observed with NAD+ | Geobacillus stearothermophilus |