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Literature summary for 1.1.1.44 extracted from

  • Veronese, F.M.; Boccu, E.; Fontana, A.
    6-Phosphogluconate dehydrogenase from Bacillus stearothermophilus (1982), Methods Enzymol., 89, 282-291.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Geobacillus stearothermophilus

General Stability

General Stability Organism
noteworthy stability to heat and protein denaturants Geobacillus stearothermophilus

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoic acid)
-
Cyberlindnera jadinii
5,5'-dithiobis(2-nitrobenzoic acid)
-
Escherichia coli
5,5'-dithiobis(2-nitrobenzoic acid) Ellman's reagent Geobacillus stearothermophilus
7-Chloro-4-nitrobenzo-2-oxa-1,3-diazole NBD-Cl Geobacillus stearothermophilus
iodoacetamide
-
Geobacillus stearothermophilus
p-hydroxymercuribenzoate
-
Geobacillus stearothermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Cyberlindnera jadinii
Mg2+
-
Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
2 * 50000, SDS-PAGE Geobacillus stearothermophilus
100000
-
-
Cyberlindnera jadinii
100000
-
-
Escherichia coli
100000
-
sucrose density gradient centrifugation Geobacillus stearothermophilus
100000
-
sedimentation equilibrium Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-phospho-D-gluconate + NADP+ Cyberlindnera jadinii
-
D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r
6-phospho-D-gluconate + NADP+ Escherichia coli enzyme specific for the oxidative pentose phosphate pathway D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r
6-phospho-D-gluconate + NADP+ Geobacillus stearothermophilus enzyme specific for the oxidative pentose phosphate pathway D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r
6-phospho-D-gluconate + NADP+ Geobacillus stearothermophilus NCA 1503 enzyme specific for the oxidative pentose phosphate pathway D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r

Organism

Organism UniProt Comment Textmining
Cyberlindnera jadinii
-
-
-
Escherichia coli
-
-
-
Geobacillus stearothermophilus
-
NCA 1503
-
Geobacillus stearothermophilus NCA 1503
-
NCA 1503
-

Purification (Commentary)

Purification (Comment) Organism
-
Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
27
-
-
Geobacillus stearothermophilus

Storage Stability

Storage Stability Organism
4°C, retains full activity for several months when stored as an ammonium sulfate suspension or in acetate buffer, pH 6 in presence of a crystal of thymol to prevent bacterial growth Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-phospho-D-gluconate + NADP+
-
Cyberlindnera jadinii D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r
6-phospho-D-gluconate + NADP+
-
Escherichia coli D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r
6-phospho-D-gluconate + NADP+
-
Geobacillus stearothermophilus D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r
6-phospho-D-gluconate + NADP+ enzyme specific for the oxidative pentose phosphate pathway Escherichia coli D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r
6-phospho-D-gluconate + NADP+ enzyme specific for the oxidative pentose phosphate pathway Geobacillus stearothermophilus D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r
6-phospho-D-gluconate + NADP+
-
Geobacillus stearothermophilus NCA 1503 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r
6-phospho-D-gluconate + NADP+ enzyme specific for the oxidative pentose phosphate pathway Geobacillus stearothermophilus NCA 1503 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 50000, SDS-PAGE Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
maximum activity at pH 8 also at 43°C Geobacillus stearothermophilus
43
-
maximum activity at pH 8 also at 25°C Geobacillus stearothermophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 60
-
Escherichia coli
65 75
-
Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
retains full activity after heating for 15 min Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Cyberlindnera jadinii
8
-
-
Geobacillus stearothermophilus

pH Range

pH Minimum pH Maximum Comment Organism
6 9
-
Geobacillus stearothermophilus