Crystallization (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
General Stability | Organism |
---|---|
noteworthy stability to heat and protein denaturants | Geobacillus stearothermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) | - |
Cyberlindnera jadinii | |
5,5'-dithiobis(2-nitrobenzoic acid) | - |
Escherichia coli | |
5,5'-dithiobis(2-nitrobenzoic acid) | Ellman's reagent | Geobacillus stearothermophilus | |
7-Chloro-4-nitrobenzo-2-oxa-1,3-diazole | NBD-Cl | Geobacillus stearothermophilus | |
iodoacetamide | - |
Geobacillus stearothermophilus | |
p-hydroxymercuribenzoate | - |
Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Cyberlindnera jadinii | |
Mg2+ | - |
Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
2 * 50000, SDS-PAGE | Geobacillus stearothermophilus |
100000 | - |
- |
Cyberlindnera jadinii |
100000 | - |
- |
Escherichia coli |
100000 | - |
sucrose density gradient centrifugation | Geobacillus stearothermophilus |
100000 | - |
sedimentation equilibrium | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-phospho-D-gluconate + NADP+ | Cyberlindnera jadinii | - |
D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
6-phospho-D-gluconate + NADP+ | Escherichia coli | enzyme specific for the oxidative pentose phosphate pathway | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
6-phospho-D-gluconate + NADP+ | Geobacillus stearothermophilus | enzyme specific for the oxidative pentose phosphate pathway | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
6-phospho-D-gluconate + NADP+ | Geobacillus stearothermophilus NCA 1503 | enzyme specific for the oxidative pentose phosphate pathway | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cyberlindnera jadinii | - |
- |
- |
Escherichia coli | - |
- |
- |
Geobacillus stearothermophilus | - |
NCA 1503 | - |
Geobacillus stearothermophilus NCA 1503 | - |
NCA 1503 | - |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
27 | - |
- |
Geobacillus stearothermophilus |
Storage Stability | Organism |
---|---|
4°C, retains full activity for several months when stored as an ammonium sulfate suspension or in acetate buffer, pH 6 in presence of a crystal of thymol to prevent bacterial growth | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-phospho-D-gluconate + NADP+ | - |
Cyberlindnera jadinii | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
6-phospho-D-gluconate + NADP+ | - |
Escherichia coli | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
6-phospho-D-gluconate + NADP+ | - |
Geobacillus stearothermophilus | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
6-phospho-D-gluconate + NADP+ | enzyme specific for the oxidative pentose phosphate pathway | Escherichia coli | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
6-phospho-D-gluconate + NADP+ | enzyme specific for the oxidative pentose phosphate pathway | Geobacillus stearothermophilus | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
6-phospho-D-gluconate + NADP+ | - |
Geobacillus stearothermophilus NCA 1503 | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
6-phospho-D-gluconate + NADP+ | enzyme specific for the oxidative pentose phosphate pathway | Geobacillus stearothermophilus NCA 1503 | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 50000, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
maximum activity at pH 8 also at 43°C | Geobacillus stearothermophilus |
43 | - |
maximum activity at pH 8 also at 25°C | Geobacillus stearothermophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 60 | - |
Escherichia coli |
65 | 75 | - |
Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
retains full activity after heating for 15 min | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Cyberlindnera jadinii |
8 | - |
- |
Geobacillus stearothermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Geobacillus stearothermophilus |