Literature summary for 1.1.1.44 extracted from

Chooback, L.; Price, N.E.; Karsten, W.E.; Nelson, J.; Sundstrom, P.; Cook, P.F.
Cloning, expression, purification, and characterization of the 6-phosphogluconate dehydrogenase from sheep liver (1998), Protein Expr. Purif., 13, 251-258.

Search for more literature for 1.1.1.44

Cloned(Commentary)

Cloned (Comment)	Organism
mRNA encoding the 51-kDa subunit of 6-phosphogluconate dehydrogenase from sheep liver reverse transcribed and amplified resulting cDNA subcloned into pQE-30 and overproduced in Escherichia coli M15	Ovis aries

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
51000	-	2 * 51000, SDS-PAGE	Ovis aries

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
6-phospho-D-gluconate + NADP+	Cyberlindnera jadinii	-	D-ribulose 5-phosphate + CO2 + NADPH + H+	-	r
6-phospho-D-gluconate + NADP+	Escherichia coli	-	D-ribulose 5-phosphate + CO2 + NADPH + H+	-	r
6-phospho-D-gluconate + NADP+	Ovis aries	-	D-ribulose 5-phosphate + CO2 + NADPH + H+	-	r
6-phospho-D-gluconate + NADP+	Escherichia coli JM109 M15	-	D-ribulose 5-phosphate + CO2 + NADPH + H+	-	r

Organism

Organism	UniProt	Comment	Textmining
Cyberlindnera jadinii	-	-	-
Escherichia coli	-	JM109, M15	-
Escherichia coli JM109 M15	-	JM109, M15	-
Ovis aries	-	sheep	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme	Ovis aries

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Ovis aries	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
4.7	-	recombinant enzyme	Ovis aries

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
6-phospho-D-gluconate + NADP+	-	Cyberlindnera jadinii	D-ribulose 5-phosphate + CO2 + NADPH + H+	-	r
6-phospho-D-gluconate + NADP+	-	Escherichia coli	D-ribulose 5-phosphate + CO2 + NADPH + H+	-	r
6-phospho-D-gluconate + NADP+	-	Ovis aries	D-ribulose 5-phosphate + CO2 + NADPH + H+	-	r
6-phospho-D-gluconate + NADP+	-	Escherichia coli JM109 M15	D-ribulose 5-phosphate + CO2 + NADPH + H+	-	r

Subunits

Subunits	Comment	Organism
dimer	-	Cyberlindnera jadinii
dimer	-	Escherichia coli
dimer	2 * 51000, SDS-PAGE	Ovis aries

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Release 2023.1 (January 2023)