Crystallization (Comment) | Organism |
---|---|
precipitate collected by centrifugation and dissolved in a minimal volume of 0.1 M potassium phosphate buffer, pH 7.5, 1 mM 2-mercaptoethanol | Gluconobacter oxydans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Gluconobacter oxydans | |
Hg2+ | - |
Gluconobacter oxydans | |
Ni2+ | - |
Gluconobacter oxydans | |
PCMB | - |
Gluconobacter oxydans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
NADP+ | pH 8.0, 25°C | Gluconobacter oxydans | |
5 | - |
D-glucose | pH 8.0, 25°C | Gluconobacter oxydans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Gluconobacter oxydans | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
4 * 40000, SDS-PAGE | Gluconobacter oxydans |
153000 | - |
gel filtration | Gluconobacter oxydans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter oxydans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sephadex, Blue-Dextran Sepharose, DEAE-Sephadex, crystallization | Gluconobacter oxydans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
140 | - |
- |
Gluconobacter oxydans |
Storage Stability | Organism |
---|---|
5°C, crystalline preparation as suspension in ammonium sulfate, stable for 6 months | Gluconobacter oxydans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-glucose + NADP+ | in the reverse reaction only 4% of the reaction occurs at neutral pH | Gluconobacter oxydans | D-glucono-1,5-lactone + NADPH + H+ | - |
r | |
beta-D-glucose + NADP+ | the enzyme is specific for NADP+ and completely inactive with NAD+ | Gluconobacter oxydans | D-glucono-1,5-lactone + NADPH + H+ | - |
r | |
D-glucose + NADP+ | - |
Gluconobacter oxydans | D-glucono-1,5-lactone + NADPH + H+ | - |
r | |
D-mannose + NADP+ | 88% of the activity with D-glucose | Gluconobacter oxydans | D-mannono-1,5-lactone + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 40000, SDS-PAGE | Gluconobacter oxydans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Gluconobacter oxydans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9 | - |
Gluconobacter oxydans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | the enzyme is specific for NADP+ and completely inactive with NAD+ | Gluconobacter oxydans | |
NADP+ | the enzyme is specific for NADP+ and completely inactive with NAD+ | Gluconobacter oxydans |