Literature summary for 1.1.1.47 extracted from

Yang, Y.; Wei, B.; Zhao, Y.; Wang, J.
Construction of an integrated enzyme system consisting azoreductase and glucose 1-dehydrogenase for dye removal (2013), Biores. Technol., 130, 517-521.

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Application

Application	Comment	Organism
biotechnology	azoreductase and glucose 1-dehydrogenase are coupled for both continuous generation of the cofactor NADH and azo dye removal. The results show that 85% maximum relative activity of azoreductase in an integrated enzyme system is obtained at the conditions: 1 U azoreductase: 10 U glucose 1-dehydrogenase, 250 mM glucose, 1.0 mM NAD+ and 150 microM methyl red	Lysinibacillus sphaericus

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Lysinibacillus sphaericus

Organism

Organism	UniProt	Comment	Textmining
Lysinibacillus sphaericus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glucose + NAD+	-	Lysinibacillus sphaericus	D-glucono-1,5-lactone + NADH + H+	-	?

Synonyms

Synonyms	Comment	Organism
glucose1-dehydrogenase	-	Lysinibacillus sphaericus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	assay at	Lysinibacillus sphaericus

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Release 2023.1 (January 2023)