Cloned (Comment) | Organism |
---|---|
expressed as a His-tagged fusion protein | Priestia megaterium |
Crystallization (Comment) | Organism |
---|---|
the crystal structures of BmGlcDH-IV in ligand-free, NADH-bound and beta-D-glucose-bound forms is determined to a resolution of 2.0 A | Priestia megaterium |
Protein Variants | Comment | Organism |
---|---|---|
A258F | Km (D-glucose) increased at pH 6 compared to wild-type, decreased at pH 8 compared to wild-type. Mutant shows higher substrate specificity with D-xylose, D-mannose, D-galactose and D-glucosamine compared to wild-type. Mutant shows a markedly deteriorated thermostability | Priestia megaterium |
DELTAG261 | Km (D-glucose) highly increased at pH 6 and pH 8 compared to wild-type.Specific activity of mutant for D-glucose is severely decreased at both pH 6.0 and pH 8.0. Mutant shows a markedly deteriorated thermostability | Priestia megaterium |
G259A | Km (D-glucose) increased at pH 6 and pH 8 compared to wild-type. Specific activity of the G259A mutant is slightly lower, but is still comparable with wild-type BmGlcDH-IV. Thermostability comparable to wild-type | Priestia megaterium |
G259V | Km (D-glucose) highly increased at pH 6 and pH 8 compared to wild-type. Specific activity of mutant for D-glucose is severely decreased at both pH 6.0 and pH 8.0. Thermostability comparable to wild-type | Priestia megaterium |
G261A | Km (D-glucose) highly increased at pH 6 and pH 8 compared to wild-type. Specific activity of mutant for D-glucose is severely decreased at both pH 6.0 and pH 8.0. Thermostability comparable to wild-type | Priestia megaterium |
G261V | Km (D-glucose) highly increased at pH 6 and pH 8 compared to wild-type. Specific activity of mutant for D-glucose is severely decreased at both pH 6.0 and pH 8.0. Thermostability comparable to wild-type | Priestia megaterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.1 | - |
D-glucose | pH 6.0, 37°C, wild-tpye | Priestia megaterium | |
4 | 10 | D-glucose | pH 6.0, 37°C, mutant G261A | Priestia megaterium | |
4.3 | - |
D-glucose | pH 6.0, 37°C, mutant A258F | Priestia megaterium | |
9.8 | - |
D-glucose | pH 8.0, 37°C, mutant A258F | Priestia megaterium | |
11 | - |
D-glucose | pH 6.0, 37°C, wild-tpye | Priestia megaterium | |
12 | - |
D-glucose | pH 8.0, 37°C, wild-tpye | Priestia megaterium | |
63 | - |
D-glucose | pH 8.0, 37°C, wild-tpye | Priestia megaterium | |
81 | - |
D-glucose | pH 6.0, 37°C, mutant G259A | Priestia megaterium | |
380 | - |
D-glucose | pH 8.0, 37°C, mutant G259A | Priestia megaterium | |
1100 | - |
D-glucose | pH 6.0, 37°C, mutant DELTAG261 | Priestia megaterium | |
1400 | - |
D-glucose | pH 6.0, 37°C, mutant G259V | Priestia megaterium | |
2000 | - |
D-glucose | pH 8.0, 37°C, mutant DELTAG261 | Priestia megaterium | |
2000 | - |
D-glucose | pH 8.0, 37°C, mutant G261A | Priestia megaterium | |
4000 | - |
D-glucose | pH 6.0, 37°C, mutant G261V | Priestia megaterium | |
4000 | - |
D-glucose | pH 8.0, 37°C, mutant G259V | Priestia megaterium | |
5000 | - |
D-glucose | pH 8.0, 37°C, mutant G261V | Priestia megaterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | - |
- |
- |
Priestia megaterium | P39485 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose + NAD+ | substrate specificity wild-tpye: 2.9% (reference: D-glucose 100%) | Priestia megaterium | D-galactono-1,5-lactone + NADH + H+ | - |
? | |
D-galactose + NAD+ | substrate specificity wild-tpye: 9.1% (reference: D-glucose 100%) | Priestia megaterium | D-galactono-1,5-lactone + NADH + H+ | - |
? | |
D-glucosamine + NAD+ | substrate specificity wild-tpye: 11% (reference: D-glucose 100%) | Priestia megaterium | D-glucosamino-1,5-lactone + NADH + H+ | - |
? | |
D-glucosamine + NAD+ | substrate specificity wild-tpye: 3% (reference: D-glucose 100%) | Priestia megaterium | D-glucosamino-1,5-lactone + NADH + H+ | - |
? | |
D-glucose + NAD+ | - |
Priestia megaterium | D-glucono-1,5-lactone + NADH + H+ | - |
? | |
D-mannose + NAD+ | substrate specificity wild-tpye: 18% (reference: D-glucose 100%) | Priestia megaterium | D-mannono-1,5-lactone + NADH + H+ | - |
? | |
D-mannose + NAD+ | substrate specificity wild-tpye: 4.8% (reference: D-glucose 100%) | Priestia megaterium | D-mannono-1,5-lactone + NADH + H+ | - |
? | |
D-xylose + NAD+ | substrate specificity wild-tpye: 10% (reference: D-glucose 100%) | Priestia megaterium | D-xylono-1,5-lactone + NADH + H+ | - |
? | |
D-xylose + NAD+ | substrate specificity wild-tpye: 35% (reference: D-glucose 100%) | Priestia megaterium | D-xylono-1,5-lactone + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BmGlcDH-III | - |
Priestia megaterium |
BmGlcDH-IV | - |
Priestia megaterium |
glucose 1-dehydrogenase III | - |
Priestia megaterium |
glucose 1-dehydrogenase IV | - |
Priestia megaterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Priestia megaterium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
wild-type enzyme retains 20% activity after 20 min at 65°C | Priestia megaterium |
65 | - |
wild-type enzyme retains 70% activity after 40 min at 65°C | Priestia megaterium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Priestia megaterium |