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Literature summary for 1.1.1.48 extracted from

  • Wengenmayer, F.; Ueberschär, K.H.; Kurz, G.
    Sulfhydryl group reactivity of D-galactose dehydrogenase from Pseudomonas saccharophila (1974), Eur. J. Biochem., 43, 49-58.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoic acid) reacts with first 4 sulfhydryl groups of enzyme, inhibition is completely reversible upon addition of reducing agents, NAD+ and NADH protect against inactivation Pelomonas saccharophila
p-chloromercuribenzoate
-
Pelomonas saccharophila

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-galactose + NAD+ Pelomonas saccharophila
-
D-galactono-1,5-lactone + NADH
-
?

Organism

Organism UniProt Comment Textmining
Pelomonas saccharophila
-
-
-

Oxidation Stability

Oxidation Stability Organism
oxidation of sulhydryl residues inactivates enzyme, reactivation with 100 mM 2-mercaptoethanol or 10 mM dithiothreitol, pH 7.0, 30°C, enzymatically active oligomers of native enzyme are formed upon storage without reducing agents Pelomonas saccharophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactose + NAD+
-
Pelomonas saccharophila D-galactono-1,5-lactone + NADH
-
?