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Literature summary for 1.1.1.49 extracted from
- D-glucose-6-phosphate dehydrogenase (Entner-Doudoroff enzyme) from Pseudomonas fluorescens. Purification, properties and regulation (1975), Eur. J. Biochem., 59, 545-559.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | - |
Pseudomonas fluorescens |  |
| ADP | - |
Pseudomonas fluorescens | |
| AMP | - |
Pseudomonas fluorescens | |
| ATP | - |
Pseudomonas fluorescens | |
| CoA | - |
Pseudomonas fluorescens | |
| GTP | - |
Pseudomonas fluorescens | |
| ITP | - |
Pseudomonas fluorescens | |
| NADH | - |
Pseudomonas fluorescens | |
| NADPH | - |
Pseudomonas fluorescens | |
| phosphoenolpyruvate | - |
Pseudomonas fluorescens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 220000 | - |
Entner-Doudoroff enzyme, gel chromatography | Pseudomonas fluorescens |
| 265000 | - |
Zwischenferment, gel chromatography | Pseudomonas fluorescens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 6-phosphate + H2O + NADP+ | Pseudomonas fluorescens | - |
D-gluconate 6-phosphate + NADPH + H+ | 6-phosphoglucono-delta-lactone | r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas fluorescens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pseudomonas fluorescens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, several months | Pseudomonas fluorescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 6-phosphate + H2O + NADP+ | - |
Pseudomonas fluorescens | D-gluconate 6-phosphate + NADPH + H+ | 6-phosphoglucono-delta-lactone | r | |
| D-glucose 6-phosphate + NAD+ | - |
Pseudomonas fluorescens | D-glucono-1,5-lactone 6-phosphate + NADH + H+ | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Pseudomonas fluorescens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1120 | - |
NADP+ | - |
Pseudomonas fluorescens | |
| 1380 | - |
NAD+ | - |
Pseudomonas fluorescens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.9 | - |
- |
Pseudomonas fluorescens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 10 | Entner-Doudoroff enzyme stable | Pseudomonas fluorescens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Pseudomonas fluorescens | |
| NADP+ | - |
Pseudomonas fluorescens | |
| NADPH | - |
Pseudomonas fluorescens | |
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