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Literature summary for 1.1.1.49 extracted from
- Cloning, expression, and characterization of the gsdA gene encoding thermophilic glucose-6-phosphate dehydrogenase from Aquifex aeolicus (2002), Extremophiles, 6, 283-289.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli as His-tagged enzyme | Aquifex aeolicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.063 | - |
D-glucose 6-phosphate | 70°C, recombinant enzyme, with NADP+ | Aquifex aeolicus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
2 * 55000, SDS-PAGE | Aquifex aeolicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 6-phosphate + NADP+ | Aquifex aeolicus | - |
D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aquifex aeolicus | - |
gene gsdA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli, to homogeneity by heat precipitation, and nickel affinity chromatography | Aquifex aeolicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 6-phosphate + NADP+ | - |
Aquifex aeolicus | D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 55000, SDS-PAGE | Aquifex aeolicus |
| More | modeling of subunit interface reveals the importance of cysteine residues for oligomeric integrity | Aquifex aeolicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| G6PDH | - |
Aquifex aeolicus |
| glucose-6-phosphate dehydrogenase | - |
Aquifex aeolicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
- |
Aquifex aeolicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
half-life: 24 h | Aquifex aeolicus |
| 100 | - |
half-life: 12 h | Aquifex aeolicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Aquifex aeolicus | |
| NADP+ | preferred cofactor, 20.4fold higher activity at 40°C and 5.7fold at 70°C than with NAD+ | Aquifex aeolicus | |
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