Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli as His-tagged enzyme | Aquifex aeolicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.063 | - |
D-glucose 6-phosphate | 70°C, recombinant enzyme, with NADP+ | Aquifex aeolicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
2 * 55000, SDS-PAGE | Aquifex aeolicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADP+ | Aquifex aeolicus | - |
D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex aeolicus | - |
gene gsdA | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli, to homogeneity by heat precipitation, and nickel affinity chromatography | Aquifex aeolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADP+ | - |
Aquifex aeolicus | D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 55000, SDS-PAGE | Aquifex aeolicus |
More | modeling of subunit interface reveals the importance of cysteine residues for oligomeric integrity | Aquifex aeolicus |
Synonyms | Comment | Organism |
---|---|---|
G6PDH | - |
Aquifex aeolicus |
glucose-6-phosphate dehydrogenase | - |
Aquifex aeolicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Aquifex aeolicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
half-life: 24 h | Aquifex aeolicus |
100 | - |
half-life: 12 h | Aquifex aeolicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Aquifex aeolicus | |
NADP+ | preferred cofactor, 20.4fold higher activity at 40°C and 5.7fold at 70°C than with NAD+ | Aquifex aeolicus |