Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.49 extracted from

  • Hansen, T.; Schlichting, B.; Schonheit, P.
    Glucose-6-phosphate dehydrogenase from the hyperthermophilic bacterium Thermotoga maritima: expression of the g6pd gene and characterization of an extremely thermophilic enzyme (2002), FEMS Microbiol. Lett., 216, 249-253.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli strain BL21 as His-tagged enzyme Thermotoga maritima

Inhibitors

Inhibitors Comment Organism Structure
ATP 15% inhibition at 2.5 mM, 24% inhibition at 10 mM Thermotoga maritima
NADPH competitive Thermotoga maritima
phosphoenolpyruvate 13% inhibition at 2.5 mM, 31% inhibition at 10 mM Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
NADP+ pH 7.4, 80°C, recombinant enzyme Thermotoga maritima
0.06
-
thio-NADP+ pH 7.4, 80°C, recombinant enzyme Thermotoga maritima
0.15
-
D-glucose 6-phosphate pH 7.4, 80°C, recombinant enzyme Thermotoga maritima
1.5
-
thio-NAD+ pH 7.4, 80°C, recombinant enzyme Thermotoga maritima
12
-
NAD+ pH 7.4, 80°C, recombinant enzyme Thermotoga maritima
35
-
2-deoxy-D-glucose 6-phosphate pH 7.4, 80°C, recombinant enzyme Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
95000
-
gel filtration, recombinant enzyme Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose 6-phosphate + NADP+ Thermotoga maritima
-
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
-
gene g6pd
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-glucose 6-phosphate + NAD(P)+ low activity Thermotoga maritima 2-deoxy-D-glucono-1,5-lactone 6-phosphate + NAD(P)H
-
?
D-glucose 6-phosphate + NAD(P)+
-
Thermotoga maritima D-glucono-1,5-lactone 6-phosphate + NAD(P)H
-
?
D-glucose 6-phosphate + NAD+
-
Thermotoga maritima D-glucono-1,5-lactone 6-phosphate + NADH + H+
-
?
D-glucose 6-phosphate + NADP+
-
Thermotoga maritima D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
?
D-glucose 6-phosphate + thio-NAD(P)+ the thio-cofactors give increased activity compared to the non-thio-cofactors Thermotoga maritima D-glucono-1,5-lactone 6-phosphate + thio-NAD(P)H
-
?
D-glucose 6-phosphate + thio-NAD+
-
Thermotoga maritima 6-phospho-D-glucono-1,5-lactone + thio-NADH + H+
-
r

Subunits

Subunits Comment Organism
dimer 2 x 60000, alpha2, recombinant enzyme, SDS-PAGE Thermotoga maritima

Synonyms

Synonyms Comment Organism
G6PD
-
Thermotoga maritima
glucose-6-phosphate dehydrogenase
-
Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
92
-
-
Thermotoga maritima

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 95
-
Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
120 min, no loss in activity Thermotoga maritima
80
-
120 min, 20% loss in activity Thermotoga maritima
95 100 120 min, almost complete loss in activity Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1100
-
NAD+ pH 7.4, 80°C, recombinant enzyme Thermotoga maritima
1800
-
2-deoxy-D-glucose 6-phosphate pH 7.4, 80°C, recombinant enzyme Thermotoga maritima
2100
-
thio-NAD+ pH 7.4, 80°C, recombinant enzyme Thermotoga maritima
3500
-
NADP+ pH 7.4, 80°C, recombinant enzyme Thermotoga maritima
3500
-
D-glucose 6-phosphate pH 7.4, 80°C, recombinant enzyme Thermotoga maritima
8000
-
thio-NADP+ pH 7.4, 80°C, recombinant enzyme Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
Thermotoga maritima

pH Range

pH Minimum pH Maximum Comment Organism
5.9 9 50% of maximal activity at pH 5.9 and pH 9.0 Thermotoga maritima

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Thermotoga maritima
NADP+ highly preferred cofactor, 1000fold higher activity than with NAD+ Thermotoga maritima
thio-NAD+
-
Thermotoga maritima
thio-NADP+ best cofactor Thermotoga maritima

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.11
-
NADPH pH 7.4, 80°C, recombinant enzyme Thermotoga maritima