Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli strain BL21 as His-tagged enzyme | Thermotoga maritima |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | 15% inhibition at 2.5 mM, 24% inhibition at 10 mM | Thermotoga maritima | |
NADPH | competitive | Thermotoga maritima | |
phosphoenolpyruvate | 13% inhibition at 2.5 mM, 31% inhibition at 10 mM | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
NADP+ | pH 7.4, 80°C, recombinant enzyme | Thermotoga maritima | |
0.06 | - |
thio-NADP+ | pH 7.4, 80°C, recombinant enzyme | Thermotoga maritima | |
0.15 | - |
D-glucose 6-phosphate | pH 7.4, 80°C, recombinant enzyme | Thermotoga maritima | |
1.5 | - |
thio-NAD+ | pH 7.4, 80°C, recombinant enzyme | Thermotoga maritima | |
12 | - |
NAD+ | pH 7.4, 80°C, recombinant enzyme | Thermotoga maritima | |
35 | - |
2-deoxy-D-glucose 6-phosphate | pH 7.4, 80°C, recombinant enzyme | Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
95000 | - |
gel filtration, recombinant enzyme | Thermotoga maritima |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADP+ | Thermotoga maritima | - |
D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
gene g6pd | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-D-glucose 6-phosphate + NAD(P)+ | low activity | Thermotoga maritima | 2-deoxy-D-glucono-1,5-lactone 6-phosphate + NAD(P)H | - |
? | |
D-glucose 6-phosphate + NAD(P)+ | - |
Thermotoga maritima | D-glucono-1,5-lactone 6-phosphate + NAD(P)H | - |
? | |
D-glucose 6-phosphate + NAD+ | - |
Thermotoga maritima | D-glucono-1,5-lactone 6-phosphate + NADH + H+ | - |
? | |
D-glucose 6-phosphate + NADP+ | - |
Thermotoga maritima | D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? | |
D-glucose 6-phosphate + thio-NAD(P)+ | the thio-cofactors give increased activity compared to the non-thio-cofactors | Thermotoga maritima | D-glucono-1,5-lactone 6-phosphate + thio-NAD(P)H | - |
? | |
D-glucose 6-phosphate + thio-NAD+ | - |
Thermotoga maritima | 6-phospho-D-glucono-1,5-lactone + thio-NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 x 60000, alpha2, recombinant enzyme, SDS-PAGE | Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
G6PD | - |
Thermotoga maritima |
glucose-6-phosphate dehydrogenase | - |
Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
92 | - |
- |
Thermotoga maritima |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 95 | - |
Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
120 min, no loss in activity | Thermotoga maritima |
80 | - |
120 min, 20% loss in activity | Thermotoga maritima |
95 | 100 | 120 min, almost complete loss in activity | Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1100 | - |
NAD+ | pH 7.4, 80°C, recombinant enzyme | Thermotoga maritima | |
1800 | - |
2-deoxy-D-glucose 6-phosphate | pH 7.4, 80°C, recombinant enzyme | Thermotoga maritima | |
2100 | - |
thio-NAD+ | pH 7.4, 80°C, recombinant enzyme | Thermotoga maritima | |
3500 | - |
NADP+ | pH 7.4, 80°C, recombinant enzyme | Thermotoga maritima | |
3500 | - |
D-glucose 6-phosphate | pH 7.4, 80°C, recombinant enzyme | Thermotoga maritima | |
8000 | - |
thio-NADP+ | pH 7.4, 80°C, recombinant enzyme | Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Thermotoga maritima |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.9 | 9 | 50% of maximal activity at pH 5.9 and pH 9.0 | Thermotoga maritima |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Thermotoga maritima | |
NADP+ | highly preferred cofactor, 1000fold higher activity than with NAD+ | Thermotoga maritima | |
thio-NAD+ | - |
Thermotoga maritima | |
thio-NADP+ | best cofactor | Thermotoga maritima |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
NADPH | pH 7.4, 80°C, recombinant enzyme | Thermotoga maritima |