Literature summary for 1.1.1.49 extracted from

Hasmann, F.A.; Cortez, D.V.; Gurpilhares, D.B.; Santos, V.C.; Roberto, I.C.; Pessoa-Junior, A.
Continuous counter-current purification of glucose-6-phosphate dehydrogenase using liquid-liquid extraction by reverse micelles (2007), Biochem. Eng. J., 34, 236-241.

No PubMed abstract available

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
continuous counter-current purification of glucose-6-phosphate dehydrogenase from Saccharomyces cerevisiae cells by liquid-liquid extraction using reverse micelles. A biocompatible reverse micellar system consisting of 0.05 M soybean lecithin (zwitterionic surfactants) in isooctane with hexanol is employed to study the influence of different flow-rates on G6PD purification. The results show that the reverse micellar system is able to remove proteins (impurities) from the cell-free. The enzyme recovery yield varies from 32% to 115% and G6PD purification factor from 1.0 to 2.2, under flow-rates ranging from 1.6:1.6 mL/min to 6.0:6.0 ml/min for both phases (micellar and aqueous phases). The steady hold-up values demonstrate that the mass transfer capability of this extraction apparatus is practically constant. The continuous counter-current purification system employed proves to be useful for purifying glucose-6-phosphate dehydrogenase and for maintaining the enzyme activity	Saccharomyces cerevisiae

Purification (Comment)

Organism

continuous counter-current purification of glucose-6-phosphate dehydrogenase from Saccharomyces cerevisiae cells by liquid-liquid extraction using reverse micelles. A biocompatible reverse micellar system consisting of 0.05 M soybean lecithin (zwitterionic surfactants) in isooctane with hexanol is employed to study the influence of different flow-rates on G6PD purification. The results show that the reverse micellar system is able to remove proteins (impurities) from the cell-free. The enzyme recovery yield varies from 32% to 115% and G6PD purification factor from 1.0 to 2.2, under flow-rates ranging from 1.6:1.6 mL/min to 6.0:6.0 ml/min for both phases (micellar and aqueous phases). The steady hold-up values demonstrate that the mass transfer capability of this extraction apparatus is practically constant. The continuous counter-current purification system employed proves to be useful for purifying glucose-6-phosphate dehydrogenase and for maintaining the enzyme activity

Saccharomyces cerevisiae

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Release 2023.1 (January 2023)