Crystallization (Comment) | Organism |
---|---|
N-terminally truncated enzyme mutant DELTA37N, sitting drop vapour diffusion method, from 20 mMTris-HCl, pH 8.0, and 5 mM D-glucose 6-phosphate, mixing of 0.002 ml reservoir solution, containing 6% PEG 400, 1.6 M ammonium sulfate, 0.1 M HEPES pH 7.5 without D-glucose 6-phosphate and 4% PEG 400, 1.8 M ammonium sulfate, 0.1 M Na HEPES pH 7.5, with D-glucose 6-phosphate, with 0.002 ml of protein solution, containing 20 mg/ml and 33 mg/ml protein, respectively, followed by equilibration against 0.09 ml of reservoir solution, 4 days, X-ray diffraction structure determination and analysis at 2.85-3.35 A resolution, molecular replacement | Trypanosoma cruzi |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a N-terminally truncated version of the enzyme glucose-6-phosphate dehydrogenase from Trypanosoma cruzi lacking the first 37 residues, mutant DELTA37N | Trypanosoma cruzi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADP+ | Trypanosoma cruzi | - |
D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | Q1WBU6 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADP+ | - |
Trypanosoma cruzi | D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
G6PDH | - |
Trypanosoma cruzi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Trypanosoma cruzi |