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Literature summary for 1.1.1.50 extracted from
- Functional expression, purification, and characterization of 3alpha-hydroxysteroid dehydrogenase/Carbonyl reductase from Comamonas testosteroni (2000), Biochem. Biophys. Res. Commun., 272, 622-628.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression as His-tagged fusion protein in Escherichia coli | Comamonas testosteroni |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0061 | - |
Fusidic acid | - |
Comamonas testosteroni |  |
| 0.0184 | - |
cholic acid | - |
Comamonas testosteroni | |
| 0.0223 | - |
5alpha-dihydrotestosterone | - |
Comamonas testosteroni | |
| 0.0311 | - |
androsterone | - |
Comamonas testosteroni | |
| 0.0422 | - |
androstanedione | - |
Comamonas testosteroni | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26400 | - |
2 * 26400, SDS-PAGE and sequence analysis | Comamonas testosteroni |
| 49400 | - |
gel filtration | Comamonas testosteroni |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Comamonas testosteroni | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant, His-tagged from Escherichia coli | Comamonas testosteroni |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.5 | - |
fusidic acid, oxidation | Comamonas testosteroni |
| 27.4 | - |
cholic acid, oxidation | Comamonas testosteroni |
| 64.5 | - |
5alpha-dihydrotestosterone, reduction | Comamonas testosteroni |
| 85.7 | - |
androstanedione, reduction | Comamonas testosteroni |
| 119 | - |
androsteone, oxidation | Comamonas testosteroni |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5alpha-dihydrotestosterone + NAD(P)H + H+ | - |
Comamonas testosteroni | 5alpha-androstan-3alpha,17beta-diol + NAD(P)+ | - |
r | |
| 5alpha-dihydrotestosterone + NAD(P)H + H+ | - |
Comamonas testosteroni | (3beta,5alpha,17beta)-androstane-3,17-diol + NAD(P)+ | - |
r | |
| 5beta-androstan-3,17-dione + NAD(P)H | - |
Comamonas testosteroni | 5beta-androstan-17one-3-ol + NAD(P)+ | - |
r | |
| androsterone + NAD(P)+ | - |
Comamonas testosteroni | 5alpha-androstan-3,17-dione + NAD(P)H | - |
r | |
| androsterone + NAD+ | - |
Comamonas testosteroni | androstanedione + NADH | - |
? | |
| cholic acid + NAD(P)+ | - |
Comamonas testosteroni | 7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NAD(P)H | - |
r | |
| fusidic acid + NAD+ | - |
Comamonas testosteroni | ? | - |
r | |
| metapyrone + NADH | - |
Comamonas testosteroni | ? | - |
? | |
| p-nitrobenzaldehyde + NAD(P)H | - |
Comamonas testosteroni | p-nitrobenzylalcohol + NAD(P)+ | - |
r | |
| p-nitrobenzylalcohol + NAD+ | - |
Comamonas testosteroni | p-nitrobenzaldehyde + NADH | - |
r | |
| progesterone + NADH | - |
Comamonas testosteroni | ? | - |
r | |
| testosterone + NAD(P)+ | - |
Comamonas testosteroni | androstenedione + NAD(P)H + H+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 26400, SDS-PAGE and sequence analysis | Comamonas testosteroni |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Comamonas testosteroni | |
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