Cloned (Comment) | Organism |
---|---|
gene betahsd, sequence comparisons, cloning and location of the TSS and promoter (126 bp) of the 3,17beta-HSD gene, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3) | Comamonas testosteroni |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a betahsd knockout mutant of Comamonas testosteroni by homologous integration, and preparation of 3,17b-HSD gene high expression mutant | Comamonas testosteroni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Comamonas testosteroni | the wild-type strain ATCC 119960 degrades steroids, such as testosterone, estradiol, or cholesterol, differences in the enzyme mutant strains, overview | ? | - |
? | |
additional information | Comamonas testosteroni ATCC 119960 | the wild-type strain ATCC 119960 degrades steroids, such as testosterone, estradiol, or cholesterol, differences in the enzyme mutant strains, overview | ? | - |
? | |
testosterone + NADP+ | Comamonas testosteroni | - |
androstenedione + NADPH + H+ | - |
r | |
testosterone + NADP+ | Comamonas testosteroni ATCC 119960 | - |
androstenedione + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Comamonas testosteroni | P19871 | - |
- |
Comamonas testosteroni ATCC 119960 | P19871 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the wild-type strain ATCC 119960 degrades steroids, such as testosterone, estradiol, or cholesterol, differences in the enzyme mutant strains, overview | Comamonas testosteroni | ? | - |
? | |
additional information | the wild-type strain ATCC 119960 degrades steroids, such as testosterone, estradiol, or cholesterol, differences in the enzyme mutant strains, overview | Comamonas testosteroni ATCC 119960 | ? | - |
? | |
testosterone + NADP+ | - |
Comamonas testosteroni | androstenedione + NADPH + H+ | - |
r | |
testosterone + NADP+ | - |
Comamonas testosteroni ATCC 119960 | androstenedione + NADPH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
3,17beta-HSD | - |
Comamonas testosteroni |
3,17beta-hydroxysteroid dehydrogenase | - |
Comamonas testosteroni |
betahsd | - |
Comamonas testosteroni |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Comamonas testosteroni | |
NADPH | - |
Comamonas testosteroni |
Organism | Comment | Expression |
---|---|---|
Comamonas testosteroni | 3,17beta-HSD gene expression is induced by testosterone, but not by estradiol and cholesterol | up |
General Information | Comment | Organism |
---|---|---|
malfunction | compared to the wild-type Comamonas testosteroni, degradation ability of testosterone and cholesterol is almost lost, and degradation of estradiol is decreased in the 3,17beta-HSD knockout mutant. Degradation of testosterone and cholesterol is obviously increased in the 3,17beta-HSD overexpression mutant. The growths in the medium with testosterone, cholesterol or estradiol are impaired in 3,17beta-HSD knockout mutant | Comamonas testosteroni |
metabolism | Comamonas testosteroni strain ATCC11996 is a gram-negative bacterium which can use steroids as carbon and energy source. 3,17beta-HSD is an enzyme which is involved in the complete oxidative degradation of the steroid skeleton, induced in the presence of these compounds in the culture medium | Comamonas testosteroni |