Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-[(3R,5S,8R,9S,10S,13S,14S)-10,13-dimethyl-2',17-dioxohexadecahydro-3'H-spiro[cyclopenta[a]phenanthrene-3,5'-[1,3]oxazolidin]-3'-yl]-3-(morpholin-4-yl)propan-2-yl cyclohexanecarboxylate | D-5-2 | Rattus norvegicus | |
androstenedione | - |
Rattus norvegicus | |
RM-532-105 | 3beta-(o-trifluoromethylphenyl-sulfonyl-dimethylpipera-zino)-androsterone, a 3 beta-dimethylpiperazino-ADT sulfonamide derivative. RM-532-105 is a better competitor of labeled enzyme substrate [14C]-4-dione than the unlabeled natural substrate 4-dione (IC50 = 341 and 337 nM) used itself as an inhibitor | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Rattus norvegicus | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
testosterone + NADP+ | Rattus norvegicus | - |
androstenedione + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
testis | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
testosterone + NADP+ | - |
Rattus norvegicus | androstenedione + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
17 beta-HSD3 | - |
Rattus norvegicus |
17beta-HSD3 | - |
Rattus norvegicus |
17beta-hydroxysteroid dehydrogenase type 3 | - |
Rattus norvegicus |
HSD17B3 | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Rattus norvegicus | |
NADPH | - |
Rattus norvegicus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000026 | - |
pH 7.4, 37°C, inhibition in recombinant HEK-293 cells overexpressing 17 beta-HSD3, overview | Rattus norvegicus | RM-532-105 | |
0.000037 | 0.000051 | pH 7.4, 37°C | Rattus norvegicus | 1-[(3R,5S,8R,9S,10S,13S,14S)-10,13-dimethyl-2',17-dioxohexadecahydro-3'H-spiro[cyclopenta[a]phenanthrene-3,5'-[1,3]oxazolidin]-3'-yl]-3-(morpholin-4-yl)propan-2-yl cyclohexanecarboxylate | |
0.0001 | - |
pH 7.4, 37°C, inhibition in wild-type rat testis, overview | Rattus norvegicus | RM-532-105 | |
0.000337 | 0.000341 | pH 7.4, 37°C | Rattus norvegicus | androstenedione |
General Information | Comment | Organism |
---|---|---|
physiological function | 17beta-hydroxysteroid dehydrogenase type 3 catalyzes the last step in the biosynthesis of the potent androgen testosterone by stereoselectively reducing the C17 ketone of 4-androstene-3,17-dione (4-dione), with NADPH as cofactor | Rattus norvegicus |