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Literature summary for 1.1.1.54 extracted from

  • Matsushima, A.; Sato, Y.; Otsuka, M.; Watanabe, T.; Yamamoto, H.; Hirata, T.
    An enone reductase from Nicotiana tabacum: cDNA cloning, expression in Escherichia coli, and reduction of enones with the recombinant proteins (2008), Bioorg. Chem., 36, 23-28.
    View publication on PubMed
Search for more literature for 1.1.1.54

Cloned(Commentary)

Cloned (Comment) Organism
GST-fusion protein expressed in Escherichia coli BL21 Nicotiana tabacum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
 (R)-pulegone native enzyme, pH 7.0, 35°C Nicotiana tabacum
1.4
-
 (R)-pulegone recombinant enzyme, pH 7.0, 35°C Nicotiana tabacum
8.3
-
 (S)-pulegone recombinant enzyme, pH 7.0, 35°C Nicotiana tabacum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36300
-
 2 * 36300, SDS-PAGE, native mass by gel filtration Nicotiana tabacum
38482
-
 2 * 38482, recombinant protein, MALDI TOF-MS, native mass by gel filtration Nicotiana tabacum
38487
-
 2 * 38487, recombinant protein, calculated from the deduced amino acid sequence, native mass by gel filtration Nicotiana tabacum
74000
-
 gel filtration Nicotiana tabacum

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum Q9SLN8
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme, recombinant enzyme purified using GST-tag Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-pulegone + NADP+ recombinant enzyme Nicotiana tabacum (1R,4R)-isomenthone + (1R,4S)-menthone + NADPH + H+ reaction is not stereospecific as with the native enzyme ?
(R)-pulegone + NADP+ native enzyme Nicotiana tabacum (1R,4R)-isomenthone + NADPH + H+
-
 ?
(S)-pulegone + NADP+ recombinant enzyme Nicotiana tabacum (1S,4S)-isomenthone + (1S,4R)-menthone + NADPH + H+
-
 ?
allyl alcohol + NADP+
-
 Nicotiana tabacum acrolein + NADPH + H+
-
 ?
additional information (R)-carvone, (S)-carvone, (1S,5S)-verbenone, (1R,5R)-verbenone, oxalacetic acid, and (2S,4S)-carveol do not serve as substrate Nicotiana tabacum ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 36300, SDS-PAGE, native mass by gel filtration Nicotiana tabacum
dimer 2 * 38482, recombinant protein, MALDI TOF-MS, native mass by gel filtration Nicotiana tabacum
dimer 2 * 38487, recombinant protein, calculated from the deduced amino acid sequence, native mass by gel filtration Nicotiana tabacum

Synonyms

Synonyms Comment Organism
allyl-ADH
-
 Nicotiana tabacum
NtRed-1
-
 Nicotiana tabacum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 assay temperature Nicotiana tabacum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8
-
 (S)-pulegone recombinant enzyme, pH 7.0, 35°C Nicotiana tabacum
3.3
-
 (R)-pulegone recombinant enzyme, pH 7.0, 35°C Nicotiana tabacum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay pH Nicotiana tabacum

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Nicotiana tabacum