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Literature summary for 1.1.1.6 extracted from

  • Nishise, H.; Nagao, A.; Tani, Y.; Yamada, H.
    Further characterization of glycerol dehydrogenase from Cellulomonas sp. NT3060 (1984), Agric. Biol. Chem., 48, 1603-1609.
No PubMed abstract available
Search for more literature for 1.1.1.6

Activating Compound

Activating Compound Comment Organism Structure
NH4+
-
 Cellulomonas sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Cellulomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
dihydroxyacetone noncompatible inhibitor, at saturated level of glycerol Cellulomonas sp.
Hydroxyacetone
-
 Cellulomonas sp.
NADH
-
 Cellulomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.385
-
 dihydroxyacetone
-
 Cellulomonas sp.
10.9
-
 glycerol
-
 Cellulomonas sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm soluble, isolation from crude cell extract Cellulomonas sp. 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ oxidation of glycerol os greatly increased Cellulomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
336000 390000 gel filtration Cellulomonas sp.
336000 390000 sedimentation equilibrium method Cellulomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycerol + NAD+ Cellulomonas sp.
-
 dihydroxyacetone + NADH + H+
-
 ?
glycerol + NAD+ Cellulomonas sp. NT3060
-
 dihydroxyacetone + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Cellulomonas sp.
-
-
-
Cellulomonas sp. NT3060
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cellulomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
128
-
-
 Cellulomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-propanediol + NAD+
-
 Cellulomonas sp. hydroxyacetone + NADH
-
 r
1,2-propanediol + NAD+
-
 Cellulomonas sp. NT3060 hydroxyacetone + NADH
-
 r
2,3-butanediol + NAD+
-
 Cellulomonas sp. 3-hydroxybutane-2-one + NADH
-
 r
2,3-butanediol + NAD+
-
 Cellulomonas sp. NT3060 3-hydroxybutane-2-one + NADH
-
 r
glycerol + NAD+
-
 Cellulomonas sp. dihydroxyacetone + NADH + H+
-
 ?
glycerol + NAD+
-
 Cellulomonas sp. NT3060 dihydroxyacetone + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
octamer 8 * 42000-43000, SDS-PAGE Cellulomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 oxidation of glycerol Cellulomonas sp.
50
-
 oxidation of butanediol Cellulomonas sp.
65
-
 oxidation of propanediol Cellulomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 reduction of hydroxyacetone Cellulomonas sp.
6
-
 reduction of dihydroxyacetone or 3-hydroxybutane-2-one Cellulomonas sp.
8 8.5 oxidation of 1,2-propanediol Cellulomonas sp.
9
-
 oxidation of glycerol or butanediol Cellulomonas sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Cellulomonas sp.
NADH
-
 Cellulomonas sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
11.1
-
 dihydroxyacetone
-
 Cellulomonas sp.
11.6
-
 Hydroxyacetone
-
 Cellulomonas sp.