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Literature summary for 1.1.1.6 extracted from

  • Burton, R.M.
    Glycerol dehydrogenase from Aerobacter aerogenes (1955), Methods Enzymol., 1, 397-400.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
NH4+
-
Klebsiella aerogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
NADH
-
Klebsiella aerogenes
0.15
-
NAD+
-
Klebsiella aerogenes
1.3
-
dihydroxyacetone
-
Klebsiella aerogenes
39
-
glycerol
-
Klebsiella aerogenes

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm soluble, isolation from crude cell extract Klebsiella aerogenes 5737
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycerol + NAD+ Klebsiella aerogenes
-
dihydroxyacetone + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Klebsiella aerogenes
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Klebsiella aerogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
82
-
-
Klebsiella aerogenes

Storage Stability

Storage Stability Organism
frozen, retains about 42% of initial activity for 2 months Klebsiella aerogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-propanediol + NAD+ as active as glycerol Klebsiella aerogenes hydroxyacetone + NADH
-
?
1,3-propanediol + NAD+ relative rate of oxidation is 37% compared to oxidation of glycerol Klebsiella aerogenes ?
-
?
1,4-butanediol + NAD+ relative activity 17% compared to oxidation of glycerol Klebsiella aerogenes ?
-
?
2,3-butanediol + NAD+ as active as glycerol Klebsiella aerogenes 3-hydroxybutane-2-one + NADH
-
?
beta-glycerophosphate + NAD+ relative activity is 2% compared to oxidation of glycerol Klebsiella aerogenes ?
-
?
diglycerol + NAD+ relative activity is 21% compared to oxidation of glycerol Klebsiella aerogenes ?
-
?
DL-alpha-glycerophosphate + NAD+ relative activity 11%compared to oxidation of glycerol Klebsiella aerogenes ?
-
?
DL-glyceraldehyde + NAD+ relative rate of reduction is 14%compared to reduction of dihydroxyacetone Klebsiella aerogenes 3-hydroxypyruvaldehyde + NADH
-
?
ethanol + NAD+ relative activity is 1% compared to oxidation of glycerol Klebsiella aerogenes acetaldehyde + NADH
-
?
ethylene glycol + NAD+ relative activity is 20% compared to oxidation of glycerol Klebsiella aerogenes ?
-
?
glycerol + NAD+
-
Klebsiella aerogenes dihydroxyacetone + NADH + H+
-
?
glycerol-alpha-monochlorohydrin + NAD+ relative rate of oxidation is 26% compared to oxidation of glycerol Klebsiella aerogenes ?
-
?
hydroxy-2-propanone + NADH relative activity is 27% compared to oxidation of glycerol Klebsiella aerogenes propylene glycol + NAD+
-
?
i-inositol + NAD+ relative activity is 18% compared to oxidation of glycerol Klebsiella aerogenes ?
-
?
isopropanol + NAD+ relative activity is 17% compared to oxidation of glycerol Klebsiella aerogenes acetone + NADH
-
?
methylglyoxal + NADH relative rate of reduction is 56% compared to reduction of dihydroxyacetone Klebsiella aerogenes lactaldehyde + NAD+
-
?
sorbitol + NAD+ relative activity is 3% compared to oxidation of glycerol Klebsiella aerogenes ?
-
?

pH Range

pH Minimum pH Maximum Comment Organism
6.8 11 the initial rate of reaction increases upon increasing the pH from pH 6.8 to 11.0, past pH 11.0 the initial rate falls rapidly, oxidation of glycerol Klebsiella aerogenes

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
25°C, 10 min, 30% loss of activity Klebsiella aerogenes
7.5
-
25°C, 10 min, maximal stability, 10% loss of activity Klebsiella aerogenes
10
-
25°C, 10 min, 40% loss of activity Klebsiella aerogenes

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Klebsiella aerogenes
NADH
-
Klebsiella aerogenes