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Literature summary for 1.1.1.6 extracted from

  • Malaoui, H.; Marczak, R.
    Separation and characterization of the 1,3-propanediol and glycerol dehydrogenase activities from Clostridium butyricum E5 wild-type and mutant D (2001), J. Appl. Microbiol., 90, 1006-1014.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
NADH pH 9.1, 37°C, wild-type enzyme Clostridium butyricum
0.12
-
NADH pH 9.1, 37°C, mutant D Clostridium butyricum
1.18
-
dihydroxyacetone pH 9.1, 37°C, wild-type enzyme Clostridium butyricum
1.18
-
dihydroxyacetone wild type enzyme, at pH 9.1 and 37°C Clostridium butyricum
2
-
dihydroxyacetone pH 9.1, 37°C, mutant D Clostridium butyricum
2
-
dihydroxyacetone mutant enzyme E5-MD, at pH 9.1 and 37°C Clostridium butyricum
4.07
-
NAD+ pH 9.7, 37°C, wild-type enzyme Clostridium butyricum
4.7
-
NAD+ pH 9.7, 37°C, mutant D Clostridium butyricum
73.3
-
glycerol pH 9.7, 37°C, mutant D 76.3 mM Clostridium butyricum
91.7
-
glycerol pH 9.7, 37°C, wild-type enzyme Clostridium butyricum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm soluble, isolation from crude cell extract Clostridium butyricum 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM, stimulates 64.5% compared to 1 mM Mn2+ Clostridium butyricum
Fe2+ 1 mM, stimulates 26.3% compared to 1 mM Mn2+ Clostridium butyricum
Mn2+ 1 mM, activates Clostridium butyricum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
181000
-
gel filtration Clostridium butyricum

Organism

Organism UniProt Comment Textmining
Clostridium butyricum
-
mutant D
-
Clostridium butyricum E5
-
mutant D
-

Purification (Commentary)

Purification (Comment) Organism
-
Clostridium butyricum

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,3-propanediol + NAD+
-
Clostridium butyricum ?
-
?
1,3-propanediol + NAD+
-
Clostridium butyricum E5 ?
-
?
2,3-butanediol + NAD+
-
Clostridium butyricum 3-hydroxybutane-2-one + NADH
-
?
2,3-butanediol + NAD+
-
Clostridium butyricum E5 3-hydroxybutane-2-one + NADH
-
?
3-hydroxypropionaldehyde + NADH
-
Clostridium butyricum propan-1,3-diol + NAD+
-
?
acetaldehyde + NADH + H+
-
Clostridium butyricum ethanol + NAD+
-
?
acetaldehyde + NADH + H+
-
Clostridium butyricum E5 ethanol + NAD+
-
?
dihydroxyacetone + NADH + H+
-
Clostridium butyricum glycerol + NAD+
-
r
DL-glyceraldehyde + NADH
-
Clostridium butyricum glycerol + NAD+
-
?
DL-glyceraldehyde + NADH
-
Clostridium butyricum E5 glycerol + NAD+
-
?
N-butyraldehyde + NADH
-
Clostridium butyricum 1-butanol + NAD+
-
?
propionaldehyde + NADH
-
Clostridium butyricum 1-propanol + NAD+
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
oxidation of glycerol, wild-type enzyme Clostridium butyricum
7.5
-
oxidation of glycerol, mutant D Clostridium butyricum
8.3
-
reduction of dihydroxyacetone, mutant D Clostridium butyricum
8.6
-
reduction of dihydroxyacetone, wild-type enzyme Clostridium butyricum