KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
NADH | pH 9.1, 37°C, wild-type enzyme | Clostridium butyricum | |
0.12 | - |
NADH | pH 9.1, 37°C, mutant D | Clostridium butyricum | |
1.18 | - |
dihydroxyacetone | pH 9.1, 37°C, wild-type enzyme | Clostridium butyricum | |
1.18 | - |
dihydroxyacetone | wild type enzyme, at pH 9.1 and 37°C | Clostridium butyricum | |
2 | - |
dihydroxyacetone | pH 9.1, 37°C, mutant D | Clostridium butyricum | |
2 | - |
dihydroxyacetone | mutant enzyme E5-MD, at pH 9.1 and 37°C | Clostridium butyricum | |
4.07 | - |
NAD+ | pH 9.7, 37°C, wild-type enzyme | Clostridium butyricum | |
4.7 | - |
NAD+ | pH 9.7, 37°C, mutant D | Clostridium butyricum | |
73.3 | - |
glycerol | pH 9.7, 37°C, mutant D 76.3 mM | Clostridium butyricum | |
91.7 | - |
glycerol | pH 9.7, 37°C, wild-type enzyme | Clostridium butyricum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | soluble, isolation from crude cell extract | Clostridium butyricum | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM, stimulates 64.5% compared to 1 mM Mn2+ | Clostridium butyricum | |
Fe2+ | 1 mM, stimulates 26.3% compared to 1 mM Mn2+ | Clostridium butyricum | |
Mn2+ | 1 mM, activates | Clostridium butyricum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
181000 | - |
gel filtration | Clostridium butyricum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium butyricum | - |
mutant D | - |
Clostridium butyricum E5 | - |
mutant D | - |
Purification (Comment) | Organism |
---|---|
- |
Clostridium butyricum |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,3-propanediol + NAD+ | - |
Clostridium butyricum | ? | - |
? | |
1,3-propanediol + NAD+ | - |
Clostridium butyricum E5 | ? | - |
? | |
2,3-butanediol + NAD+ | - |
Clostridium butyricum | 3-hydroxybutane-2-one + NADH | - |
? | |
2,3-butanediol + NAD+ | - |
Clostridium butyricum E5 | 3-hydroxybutane-2-one + NADH | - |
? | |
3-hydroxypropionaldehyde + NADH | - |
Clostridium butyricum | propan-1,3-diol + NAD+ | - |
? | |
acetaldehyde + NADH + H+ | - |
Clostridium butyricum | ethanol + NAD+ | - |
? | |
acetaldehyde + NADH + H+ | - |
Clostridium butyricum E5 | ethanol + NAD+ | - |
? | |
dihydroxyacetone + NADH + H+ | - |
Clostridium butyricum | glycerol + NAD+ | - |
r | |
DL-glyceraldehyde + NADH | - |
Clostridium butyricum | glycerol + NAD+ | - |
? | |
DL-glyceraldehyde + NADH | - |
Clostridium butyricum E5 | glycerol + NAD+ | - |
? | |
N-butyraldehyde + NADH | - |
Clostridium butyricum | 1-butanol + NAD+ | - |
? | |
propionaldehyde + NADH | - |
Clostridium butyricum | 1-propanol + NAD+ | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
oxidation of glycerol, wild-type enzyme | Clostridium butyricum |
7.5 | - |
oxidation of glycerol, mutant D | Clostridium butyricum |
8.3 | - |
reduction of dihydroxyacetone, mutant D | Clostridium butyricum |
8.6 | - |
reduction of dihydroxyacetone, wild-type enzyme | Clostridium butyricum |