Cloned (Comment) | Organism |
---|---|
gene gldA from Escherichia coli strain JM109, expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3), subcloning in Escherichia coli TOP10 cells | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic study with different effectors, overview | Escherichia coli | |
0.05 | - |
NADH | pH 6.5, 30°C, recombinant enzyme | Escherichia coli | |
0.07 | - |
NADH | pH 6.5, 30°C, recombinant enzyme, in presence of 20 mM KCl | Escherichia coli | |
0.08 | - |
NADH | pH 6.5, 30°C, recombinant enzyme, in presence of 30 mM NH4Cl | Escherichia coli | |
0.15 | - |
Glycerone | pH 6.5, 30°C, recombinant enzyme | Escherichia coli | |
0.22 | - |
Glycerone | pH 6.5, 30°C, recombinant enzyme, in presence of 30 mM NH4Cl | Escherichia coli | |
0.24 | - |
Glycerone | pH 6.5, 30°C, recombinant enzyme, in presence of 20 mM KCl | Escherichia coli | |
0.81 | - |
NAD+ | pH 10.3, 30°C, recombinant enzyme | Escherichia coli | |
1 | - |
NAD+ | pH 10.3, 30°C, recombinant enzyme, in presence of 30 mM NH4Cl | Escherichia coli | |
3.2 | - |
NAD+ | pH 10.3, 30°C, recombinant enzyme, in presence of 20 mM KCl | Escherichia coli | |
47 | - |
glycerol | pH 10.3, 30°C, recombinant enzyme, in presence of 20 mM KCl | Escherichia coli | |
76 | - |
glycerol | pH 10.3, 30°C, recombinant enzyme | Escherichia coli | |
81 | - |
glycerol | pH 10.3, 30°C, recombinant enzyme, in presence of 30 mM NH4Cl | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | activates, less effective than NH4+ | Escherichia coli | |
NH4+ | activates, 4fold increase in Vmax produced by NH4Cl in the direction of glycerol oxidation, and 70fold reduction in the Km for dihydroxyaceton and 2fold increase in the Vmax with 30 mM NH4Cl added to the dihydroxyaceton reduction reaction | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39800 | - |
8 * 40000, recombinant His-tagged enzyme, SDS-PAGE, 8 * 39800, about, sequence calculation | Escherichia coli |
40000 | - |
8 * 40000, recombinant His-tagged enzyme, SDS-PAGE, 8 * 39800, about, sequence calculation | Escherichia coli |
320000 | - |
recombinant His-tagged enzyme, gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerol + NAD+ | Escherichia coli | - |
glycerone + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene gldA | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by immobilized metal affinity chromatography or by heat shock treatment, both resulting in about the same protein purity of over 90% | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerol + NAD+ | - |
Escherichia coli | glycerone + NADH + H+ | - |
r | |
additional information | ability of the enzyme to use glycerol from biodiesel waste as substrate, overview | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 40000, recombinant His-tagged enzyme, SDS-PAGE, 8 * 39800, about, sequence calculation | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
Gro dehydrogenase | - |
Escherichia coli |
GroDHase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Escherichia coli |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 80 | activity range, profile overview | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
dihydroxyaceton reduction reaction | Escherichia coli |
11 | - |
glycerol oxidation reaction | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | dihydroxyaceton reduction reaction, activity range, profile overview | Escherichia coli |
7.5 | 12 | glycerol oxidation reaction, activity range, profile overview | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Escherichia coli | |
NADH | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the medium-chain dehydrogenase/reductase, MDRase, superfamily | Escherichia coli |
metabolism | in Escherichia coli, the enzyme catalyzes the first step in fermentative glycerol metabolism to produce dihydroxyacetone, biochemical transformation pathway of glycerol, overview | Escherichia coli |
physiological function | GroDHase is mainly involved in Gro utilization as a carbon and energy source | Escherichia coli |