Application | Comment | Organism |
---|---|---|
analysis | the recombinant chimeric fusion enzyme GDH-NOX has a potential application for quick glycerol analysis and dioxyacetone biosynthesis | Klebsiella pneumoniae |
Cloned (Comment) | Organism |
---|---|
recombinant expression of the His-tagged enzyme encoding gene fused to the codon-optimized NADH oxidase gene nox from Lactobacillus brevis, chimeric enzyme GDH-NOX, in Escherichia coli strain BL21(DE3) resulting in bioconversion of glycerol to dihydroxyacetone in the cells | Klebsiella pneumoniae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the glycerol dehydrogenase gene from Klebsiella pneumoniae is fused to codon-optimized NADH oxidase gene from Lactobacillus brevis. Gene fusion of glycerol dehydrogenase (GDH) and NOX forms a bifunctional multienzyme for bioconversion of glycerol coupled with coenzyme regeneration | Klebsiella pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0513 | - |
NADH | recombinant chimeric enzyme GDH-NOX, pH 11.0, 37°C | Klebsiella pneumoniae | |
19.4 | - |
glycerol | recombinant chimeric enzyme GDH-NOX, pH 11.0, 37°C | Klebsiella pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerol + NAD+ | Klebsiella pneumoniae | - |
glycerone + NADH + H+ | - |
r | |
glycerol + NAD+ | Klebsiella pneumoniae DSM 2026 | - |
glycerone + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | B2ZPN8 | - |
- |
Klebsiella pneumoniae DSM 2026 | B2ZPN8 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged chimeric enzyme GDH-NOX from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Klebsiella pneumoniae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15.7 | - |
recombinant His-tagged chimeric enzyme GDH-NOX, pH 11.0, 37°C | Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerol + NAD+ | - |
Klebsiella pneumoniae | glycerone + NADH + H+ | - |
r | |
glycerol + NAD+ | - |
Klebsiella pneumoniae DSM 2026 | glycerone + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
GDH | - |
Klebsiella pneumoniae |
GldA | - |
Klebsiella pneumoniae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
11 | - |
recombinant His-tagged chimeric enzyme GDH-NOX | Klebsiella pneumoniae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
11 | - |
recombinant His-tagged chimeric enzyme GDH-NOX | Klebsiella pneumoniae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Klebsiella pneumoniae | |
NADH | - |
Klebsiella pneumoniae |