Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM, 82% inhibition | Homo sapiens | |
iodoacetamide | 1 mM, 78% inhibition | Homo sapiens | |
Mg2+ | 1 mM, 18% inhibition | Homo sapiens | |
PCMB | 1 mM, complete inhibition | Homo sapiens | |
Zn2+ | 1 mM, 33% inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0032 | - |
estradiol-17beta | cosubstrate NADP+, secretory endometrium | Homo sapiens | |
0.0036 | - |
estradiol | cosubstrate NAD+, secretory endometrium | Homo sapiens | |
0.0128 | - |
estradiol-17beta | cosubstrate NADP+, proliferating endometrium | Homo sapiens | |
0.0134 | - |
estradiol | cosubstrate NAD+, proliferating endometrium | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial outer membrane | - |
Homo sapiens | 5741 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
uterine endometrium | proliferative | Homo sapiens | - |
uterine endometrium | secretory | Homo sapiens | - |
uterine endometrium | endometrial carcinoma | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-androstene-3,17-dione + NADPH + H+ | - |
Homo sapiens | testosterone + NADP+ | - |
r | |
androstenedione + NADPH + H+ | 50% of the activity with estradiol-17beta | Homo sapiens | testosterone + NADP+ | - |
? | |
estradiol-17beta + NAD+ | - |
Homo sapiens | estrone + NADH | - |
r | |
estradiol-17beta + NADP+ | - |
Homo sapiens | estrone + NADPH | - |
r | |
estrone + NADH | - |
Homo sapiens | estradiol-17beta + NAD+ | - |
r | |
estrone + NADPH + H+ | - |
Homo sapiens | estradiol-17beta + NADP+ | - |
r | |
testosterone + NADP+ | 75% of the activity with estradiol-17beta | Homo sapiens | 4-androstene-3,17-dione + NADPH + H+ | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
enzyme from crude washed mitochondria | Homo sapiens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
24 | 54 | 24°C: about 60% of maximal activity, 54°C: about 50% of maximal activity, enzyme from crude washed mitochondria | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
reduction of estrone | Homo sapiens |
9.5 | - |
oxidation of estradiol-17beta | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | cofactor | Homo sapiens | |
NADH | cofactor | Homo sapiens | |
NADP+ | cofactor | Homo sapiens | |
NADPH | cofactor | Homo sapiens |