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Literature summary for 1.1.1.65 extracted from

  • Nakano, M.; Morita, T.; Yamamoto, T.; Sano, H.; Ashiuchi, M.; Masui, R.; Kuramitsu, S.; Toshiharu, Y.
    Purification, molecular cloning, and catalytic activity of Schizosaccharomyces pombe pyridoxal reductase (1999), J. Biol. Chem., 274, 23185-23190.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Schizosaccharomyces pombe

General Stability

General Stability Organism
purified enzyme very unstable but can be stabilized by low concentrations of various detergents such as Tween 40 Schizosaccharomyces pombe

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.096
-
2-nitrobenzaldehyde
-
Schizosaccharomyces pombe
0.9
-
pyridoxal
-
Schizosaccharomyces pombe

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
gel filtration Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyridoxine + NADP+ Schizosaccharomyces pombe
-
pyridoxal + NADPH
-
r

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe O14295
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Schizosaccharomyces pombe

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrobenzaldehyde + NADPH
-
Schizosaccharomyces pombe 2-nitrobenzyl alcohol + NADP+
-
?
pyridoxine + NADP+
-
Schizosaccharomyces pombe pyridoxal + NADPH
-
r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.5 for formation of pyridoxine Schizosaccharomyces pombe
7.5 8.5 for formation of pyridoxal Schizosaccharomyces pombe

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Schizosaccharomyces pombe
NADPH
-
Schizosaccharomyces pombe