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Literature summary for 1.1.1.67 extracted from

  • Schneider, K.H.; Giffhorn, F.
    Purification and properties of a polyol dehydrogenase from the phototrophic bacterium Rhodobacter sphaeroides (1989), Eur. J. Biochem., 184, 15-19.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01 0.079 NADH
-
Cereibacter sphaeroides
0.18 0.27 NAD+
-
Cereibacter sphaeroides
0.29 21.8 D-mannitol
-
Cereibacter sphaeroides
1.8 6.5 D-arabinitol
-
Cereibacter sphaeroides
16.3 79.2 D-fructose
-
Cereibacter sphaeroides
32
-
D-glucitol
-
Cereibacter sphaeroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47200
-
sucrose density gradient centrifugation Cereibacter sphaeroides
52200
-
1 * 52200, SDS-PAGE   Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-mannitol + NAD+ Cereibacter sphaeroides
-
D-fructose + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
phototrophic bacterium
-

Purification (Commentary)

Purification (Comment) Organism
-
Cereibacter sphaeroides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
60.3
-
-
Cereibacter sphaeroides

Storage Stability

Storage Stability Organism
4°C, 1 month Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-arabinitol + NAD+
-
Cereibacter sphaeroides D-ribulose + NADH + H+
-
?
D-glucitol + NAD+
-
Cereibacter sphaeroides ? + NADH
-
?
D-mannitol + NAD+
-
Cereibacter sphaeroides D-fructose + NADH + H+
-
?
D-mannitol + NAD+
-
Cereibacter sphaeroides D-fructose + NADH + H+
-
r
L-sorbitol + NAD+
-
Cereibacter sphaeroides L-sorbose + NADH + H+
-
?

Subunits

Subunits Comment Organism
monomer 1 * 52200, SDS-PAGE   Cereibacter sphaeroides

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
not stable above Cereibacter sphaeroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7.2 D-fructose reduction Cereibacter sphaeroides
8.9 10 D-mannitol oxidation Cereibacter sphaeroides

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Cereibacter sphaeroides
NADH
-
Cereibacter sphaeroides