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Literature summary for 1.1.1.67 extracted from

  • Parmentier, S.; Beauprez, J.; Arnaut, F.; Soetaert, W.; Vandamme, E.J.
    Gluconobacter oxydans NAD-dependent, D-fructose reducing, polyol dehydrogenases activity: screening, medium optimisation and application for enzymatic polyol production (2005), Biotechnol. Lett., 27, 305-311.
    View publication on PubMed
Search for more literature for 1.1.1.67

Activating Compound

Activating Compound Comment Organism Structure
EDTA 13% stimulation of D-fructose reduction Gluconobacter oxydans
additional information no clear stimulatory effect of MgSO4+, MnSO4, CuSO4, ZnSO4, Na2SO4 and CaSO4 on activity Gluconobacter oxydans

Inhibitors

Inhibitors Comment Organism Structure
CuSO4 1 mM, 28% inhibition of D-fructose reduction, 18% inhibition of D-sorbitol oxidation Gluconobacter oxydans
additional information no inhibitory effect on D-fructose reduction detected for MgSO4, CaSO4, Na2SO4, ZnSO4 and MnSO4 Gluconobacter oxydans

Organism

Organism UniProt Comment Textmining
Gluconobacter oxydans
-
-
-
Gluconobacter oxydans LMG 1489
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose + NADH + H+
-
 Gluconobacter oxydans D-mannitol + NAD+
-
 ?
D-fructose + NADH + H+
-
 Gluconobacter oxydans LMG 1489 D-mannitol + NAD+
-
 ?

Synonyms

Synonyms Comment Organism
mannitol dehydrogenase
-
 Gluconobacter oxydans
polyol dehydrogenase
-
 Gluconobacter oxydans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 40 The enzyme is very stable at 30°C. The half life time at 40°C is 35 min for D-mannitol oxidation. Gluconobacter oxydans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Gluconobacter oxydans

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Gluconobacter oxydans
NADH
-
 Gluconobacter oxydans