Literature summary for 1.1.1.67 extracted from

Krahulec, S.; Armao, G.C.; Bubner, P.; Klimacek, M.; Nidetzky, B.
Polyol-specific long-chain dehydrogenases/reductases of mannitol metabolism in Aspergillus fumigatus: biochemical characterization and pH studies of mannitol 2-dehydrogenase and mannitol-1-phosphate 5-dehydrogenase (2009), Chem. Biol. Interact., 178, 274-282.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli JM109 cells	Aspergillus fumigatus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.019	-	NADH	at 25°C, in 100 mM Tris/HCl buffer, pH 7.1	Aspergillus fumigatus
0.15	-	NAD+	at 25°C, in 100 mM glycine/NaOH buffer pH 10.0	Aspergillus fumigatus
13	-	D-mannitol	at 25°C, in 100 mM glycine/NaOH buffer pH 10.0	Aspergillus fumigatus
60	-	D-fructose	at 25°C, in 100 mM Tris/HCl buffer, pH 7.1	Aspergillus fumigatus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	does not require metals for activity	Aspergillus fumigatus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
58000	-	recombinant enzyme, SDS-PAGE	Aspergillus fumigatus

Organism

Organism	UniProt	Comment	Textmining
Aspergillus fumigatus	Q4WQY4	-	-

Purification (Commentary)

Purification (Comment)	Organism
Co2+ chelating Sepharose column chromatography and Superdex 200 gel filtration	Aspergillus fumigatus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
14	-	crude extract, at 25°C, using 100 mM Tris-HCl buffer, pH 7.1	Aspergillus fumigatus
33	-	after 6.7fold purification, at 25°C, using 100 mM Tris-HCl buffer, pH 7.1	Aspergillus fumigatus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-mannitol + NAD+	-	Aspergillus fumigatus	D-fructose + NADH + H+	-	r
D-mannitol + NADP+	M2DH is a much poorer enzyme when it employes NADP+ and NADPH as compared to NAD+ and NADH	Aspergillus fumigatus	D-fructose + NADPH + H+	-	r

Subunits

Subunits	Comment	Organism
monomer	1 * 58000, recombinant enzyme, SDS-PAGE	Aspergillus fumigatus

Synonyms

Synonyms	Comment	Organism
M2DH	-	Aspergillus fumigatus

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	10	-	Aspergillus fumigatus

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	dependent on, M2DH is a much poorer enzyme when it employes NADP+ and NADPH as compared to NAD+ and NADH	Aspergillus fumigatus
NADP+	M2DH is a much poorer enzyme when it employes NADP+ and NADPH as compared to NAD+ and NADH	Aspergillus fumigatus

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Release 2023.1 (January 2023)