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Literature summary for 1.1.1.71 extracted from

  • Stekhanova, T.N.; Mardanov, A.V.; Bezsudnova, E.Y.; Gumerov, V.M.; Ravin, N.V.; Skryabin, K.G.; Popov, V.O.
    Characterization of a thermostable short-chain alcohol dehydrogenase from the hyperthermophilic archaeon Thermococcus sibiricus (2010), Appl. Environ. Microbiol., 76, 4096-4098.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Thermococcus sibiricus
expression in Escherichia coli Thermococcus sibiricus

Inhibitors

Inhibitors Comment Organism Structure
acetonitrile complete inhibition in buffer without NaCl and in buffer with 600 mM NaCl Thermococcus sibiricus
chloroform 21% inhibition in buffer without NaCl, 19% inhibition in buffer with 600 mM NaCl Thermococcus sibiricus
Dimethyl formamide 87% inhibition in buffer without NaCl, 59% inhibition in buffer with 600 mM NaCl Thermococcus sibiricus
dimethyl sulfoxide complete inhibition in buffer without NaCl, 60% inhibition in buffer with 600 mM NaCl Thermococcus sibiricus
EDTA 5 mM, 20% inhibition; 5 mM, 80% of initial activity Thermococcus sibiricus
ethyl acetate complete inhibition in buffer without NaCl, 67% inhibition in buffer with 600 mM NaCl Thermococcus sibiricus
Fe2+ 10 mM, 74% of initial activity Thermococcus sibiricus
FeSO4 10 mM, 26% inhibition Thermococcus sibiricus
methanol 75% inhibition in buffer without NaCl, 81% inhibition in buffer with 600 mM NaCl Thermococcus sibiricus
Mg2+ 10 mM, 78% of initial activity Thermococcus sibiricus
n-decane 9% inhibition in buffer without NaCl, slight activation in buffer with 600 mM NaCl Thermococcus sibiricus
n-hexane 40% inhibition in buffer without NaCl, slight activation in buffer with 600 mM NaCl Thermococcus sibiricus
Zn2+ 10 mM, 79% of initial activity Thermococcus sibiricus
ZnSO4 10 mM, 21% inhibition Thermococcus sibiricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
NADPH pH 7.5, 60°C Thermococcus sibiricus
0.02
-
NADPH 60°C, pH 7.5 Thermococcus sibiricus
0.022
-
NADP+ 60°C, pH 10.5 Thermococcus sibiricus
0.022
-
NADP+ pH 10.5, 60°C Thermococcus sibiricus
17.75
-
Pyruvaldehyde pH 7.5, 60°C Thermococcus sibiricus
17.75
-
Pyruvaldehyde 60°C, pH 7.5 Thermococcus sibiricus
54.4
-
D-xylose 60°C, pH 10.5 Thermococcus sibiricus
54.4
-
D-xylose pH 10.5, 60°C Thermococcus sibiricus
168
-
2-propanol 60°C, pH 10.5 Thermococcus sibiricus
168
-
2-propanol pH 10.5, 60°C Thermococcus sibiricus

Metals/Ions

Metals/Ions Comment Organism Structure
KCl activity increases in the presence of KCl is maintained even at concentration of 3 M Thermococcus sibiricus
KCl 600 mM, 147% of initial activity Thermococcus sibiricus
additional information metal independency is supported by the absence of a significant effect of TsAdh319 preincubation with 10 mM Me2+ for 30 min before measuring the activity in the presence of 1 mM Me2+ or EDTA Thermococcus sibiricus
NaCl activity increases in the presence of NaCl is maintained even at concentration of 4 M Thermococcus sibiricus
NaCl 400 mM, 206% of initial activity Thermococcus sibiricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26200
-
2 * 26200, calculated from sequence Thermococcus sibiricus
28700
-
2 * 28700, SDS-PAGE Thermococcus sibiricus
56000 60000 gel filtration Thermococcus sibiricus

Organism

Organism UniProt Comment Textmining
Thermococcus sibiricus C6A190
-
-
Thermococcus sibiricus DSM 12597 C6A190
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermococcus sibiricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-2-butanol + NADP+ 196% relative activity as compared to 2-propanol Thermococcus sibiricus butanone + NADPH
-
?
(S)-2-butanol + NADP+ 196% of the activity with 2-propanol, 2fold preference for (S)-2-butanol over (RS)-2-butanol Thermococcus sibiricus butanone + NADPH + H+
-
?
1,3-butanediol + NADP+ 91% relative activity as compared to 2-propanol Thermococcus sibiricus ? + NADPH + H+
-
?
1-butanol + NADP+ 80% relative activity as compared to 2-propanol Thermococcus sibiricus butanal + NADPH
-
?
1-phenylmethanol + NADP+ 180% relative activity as compared to 2-propanol Thermococcus sibiricus benzaldehyde + NADPH + H+
-
?
2-pentanol + NADP+ 67% relative activity as compared to 2-propanol Thermococcus sibiricus 2-pentanone + NADPH + H+
-
?
2-pentanol + NADP+ 67% of the activity with 2-propanol Thermococcus sibiricus pentan-2-one + NADPH + H+
-
?
2-propanol + NADP+
-
Thermococcus sibiricus acetone + NADPH
-
?
2-propanol + NADP+
-
Thermococcus sibiricus acetone + NADPH + H+
-
?
3-methyl-2-pentanone + NADPH + H+ 13% relative activity as compared to pyruvaldehyde Thermococcus sibiricus ? + NADP+
-
?
benzyl alcohol + NADP+ 180% of the activity with 2-propanol Thermococcus sibiricus benzaldehyde + NADPH + H+
-
?
cellobiose + NADP+ 71% relative activity as compared to 2-propanol Thermococcus sibiricus ? + NADPH + H+
-
?
cellobiose + NADP+ 71% of the activity with 2-propanol Thermococcus sibiricus ? + NADPH + H+
-
?
D-arabinose + NADP+ 200% relative activity as compared to 2-propanol Thermococcus sibiricus ? + NADPH + H+
-
?
D-arabinose + NADP+ 200% of the activity with 2-propanol. D-arabinose is preferred over L-arabinose Thermococcus sibiricus D-arabitol + NADPH + H+
-
?
D-glucose + NADP+ 146% relative activity as compared to 2-propanol Thermococcus sibiricus ? + NADPH + H+
-
?
D-glucose + NADP+ 146% of the activity with 2-propanol Thermococcus sibiricus ? + NADPH + H+
-
?
D-mannose + NADP+ 48% relative activity as compared to 2-propanol Thermococcus sibiricus ? + NADPH + H+
-
?
D-ribose + NADP+ 35% relative activity as compared to 2-propanol Thermococcus sibiricus ? + NADPH + H+
-
?
D-xylose + NADP+ 246% relative activity as compared to 2-propanol Thermococcus sibiricus ? + NADPH + H+
-
?
D-xylose + NADP+ 246% of the activity with 2-propanol Thermococcus sibiricus ? + NADPH + H+
-
?
dimethylglyoxal + NADPH + H+ 270% relative activity as compared to pyruvaldehyde Thermococcus sibiricus ? + NADP+
-
?
dimethylglyoxal + NADPH + H+ 270% of the activity with pyruvaldehyde Thermococcus sibiricus ? + NADP+
-
?
ethanol + NADP+ 36% relative activity as compared to 2-propanol Thermococcus sibiricus acetaldehyde + NADPH + H+
-
?
glycerol + NADP+ 16% relative activity as compared to 2-propanol Thermococcus sibiricus glyceraldehyde + NADPH + H+
-
?
glyoxylic acid + NADPH + H+ 36% relative activity as compared to pyruvaldehyde Thermococcus sibiricus ? + NADP+
-
?
L-arabinose + NADP+ 17% relative activity as compared to 2-propanol Thermococcus sibiricus ? + NADPH + H+
-
?
additional information no oxidation of methanol, methoxyethanol, ethylene glycol, or D-galactose. No reduction of acetone, cyclopentanone, D-arabinose, D-xylose, D-glucose or cellobiose Thermococcus sibiricus ?
-
?
pyruvaldehyde + NADPH + H+
-
Thermococcus sibiricus lactaldehyde + NADP+
-
?
pyruvaldehyde + NADPH + H+
-
Thermococcus sibiricus ? + NADP+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 26200, calculated from sequence Thermococcus sibiricus
dimer 2 * 28700, SDS-PAGE Thermococcus sibiricus

Synonyms

Synonyms Comment Organism
Tsib_0319 locus name Thermococcus sibiricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
assay at Thermococcus sibiricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
2 h, 10% loss of activity Thermococcus sibiricus
70
-
1.5 h, no loss of activity Thermococcus sibiricus
80
-
2 h, 15% loss of activity Thermococcus sibiricus
80
-
1.5 h, no loss of activity Thermococcus sibiricus
90
-
half-life: 2 h Thermococcus sibiricus
90
-
half-life 2 h Thermococcus sibiricus
100
-
half-life: 1 h Thermococcus sibiricus
100
-
half-life 1 h Thermococcus sibiricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.45
-
NADP+ 60°C, pH 10.5 Thermococcus sibiricus
0.45
-
NADP+ pH 10.5, 60°C Thermococcus sibiricus
0.53
-
2-propanol 60°C, pH 10.5 Thermococcus sibiricus
0.53
-
2-propanol pH 10.5, 60°C Thermococcus sibiricus
0.7
-
D-xylose 60°C, pH 10.5 Thermococcus sibiricus
0.7
-
D-xylose pH 10.5, 60°C Thermococcus sibiricus
1.51
-
NADPH pH 7.5, 60°C Thermococcus sibiricus
1.51
-
NADPH 60°C, pH 7.5 Thermococcus sibiricus
2.04
-
Pyruvaldehyde pH 7.5, 60°C Thermococcus sibiricus
2.04
-
Pyruvaldehyde 60°C, pH 7.5 Thermococcus sibiricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at, reduction of ketones Thermococcus sibiricus
10.5
-
assay at, oxidation of alcohols Thermococcus sibiricus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Thermococcus sibiricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.003
-
2-propanol 60°C, pH 10.5 Thermococcus sibiricus
0.013
-
D-xylose 60°C, pH 10.5 Thermococcus sibiricus
0.11
-
Pyruvaldehyde 60°C, pH 7.5 Thermococcus sibiricus
20.45
-
NADP+ 60°C, pH 10.5 Thermococcus sibiricus
75.5
-
NADPH 60°C, pH 7.5 Thermococcus sibiricus