Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Thermococcus sibiricus |
expression in Escherichia coli | Thermococcus sibiricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetonitrile | complete inhibition in buffer without NaCl and in buffer with 600 mM NaCl | Thermococcus sibiricus | |
chloroform | 21% inhibition in buffer without NaCl, 19% inhibition in buffer with 600 mM NaCl | Thermococcus sibiricus | |
Dimethyl formamide | 87% inhibition in buffer without NaCl, 59% inhibition in buffer with 600 mM NaCl | Thermococcus sibiricus | |
dimethyl sulfoxide | complete inhibition in buffer without NaCl, 60% inhibition in buffer with 600 mM NaCl | Thermococcus sibiricus | |
EDTA | 5 mM, 20% inhibition; 5 mM, 80% of initial activity | Thermococcus sibiricus | |
ethyl acetate | complete inhibition in buffer without NaCl, 67% inhibition in buffer with 600 mM NaCl | Thermococcus sibiricus | |
Fe2+ | 10 mM, 74% of initial activity | Thermococcus sibiricus | |
FeSO4 | 10 mM, 26% inhibition | Thermococcus sibiricus | |
methanol | 75% inhibition in buffer without NaCl, 81% inhibition in buffer with 600 mM NaCl | Thermococcus sibiricus | |
Mg2+ | 10 mM, 78% of initial activity | Thermococcus sibiricus | |
n-decane | 9% inhibition in buffer without NaCl, slight activation in buffer with 600 mM NaCl | Thermococcus sibiricus | |
n-hexane | 40% inhibition in buffer without NaCl, slight activation in buffer with 600 mM NaCl | Thermococcus sibiricus | |
Zn2+ | 10 mM, 79% of initial activity | Thermococcus sibiricus | |
ZnSO4 | 10 mM, 21% inhibition | Thermococcus sibiricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
NADPH | pH 7.5, 60°C | Thermococcus sibiricus | |
0.02 | - |
NADPH | 60°C, pH 7.5 | Thermococcus sibiricus | |
0.022 | - |
NADP+ | 60°C, pH 10.5 | Thermococcus sibiricus | |
0.022 | - |
NADP+ | pH 10.5, 60°C | Thermococcus sibiricus | |
17.75 | - |
Pyruvaldehyde | pH 7.5, 60°C | Thermococcus sibiricus | |
17.75 | - |
Pyruvaldehyde | 60°C, pH 7.5 | Thermococcus sibiricus | |
54.4 | - |
D-xylose | 60°C, pH 10.5 | Thermococcus sibiricus | |
54.4 | - |
D-xylose | pH 10.5, 60°C | Thermococcus sibiricus | |
168 | - |
2-propanol | 60°C, pH 10.5 | Thermococcus sibiricus | |
168 | - |
2-propanol | pH 10.5, 60°C | Thermococcus sibiricus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | activity increases in the presence of KCl is maintained even at concentration of 3 M | Thermococcus sibiricus | |
KCl | 600 mM, 147% of initial activity | Thermococcus sibiricus | |
additional information | metal independency is supported by the absence of a significant effect of TsAdh319 preincubation with 10 mM Me2+ for 30 min before measuring the activity in the presence of 1 mM Me2+ or EDTA | Thermococcus sibiricus | |
NaCl | activity increases in the presence of NaCl is maintained even at concentration of 4 M | Thermococcus sibiricus | |
NaCl | 400 mM, 206% of initial activity | Thermococcus sibiricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26200 | - |
2 * 26200, calculated from sequence | Thermococcus sibiricus |
28700 | - |
2 * 28700, SDS-PAGE | Thermococcus sibiricus |
56000 | 60000 | gel filtration | Thermococcus sibiricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus sibiricus | C6A190 | - |
- |
Thermococcus sibiricus DSM 12597 | C6A190 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermococcus sibiricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-butanol + NADP+ | 196% relative activity as compared to 2-propanol | Thermococcus sibiricus | butanone + NADPH | - |
? | |
(S)-2-butanol + NADP+ | 196% of the activity with 2-propanol, 2fold preference for (S)-2-butanol over (RS)-2-butanol | Thermococcus sibiricus | butanone + NADPH + H+ | - |
? | |
1,3-butanediol + NADP+ | 91% relative activity as compared to 2-propanol | Thermococcus sibiricus | ? + NADPH + H+ | - |
? | |
1-butanol + NADP+ | 80% relative activity as compared to 2-propanol | Thermococcus sibiricus | butanal + NADPH | - |
? | |
1-phenylmethanol + NADP+ | 180% relative activity as compared to 2-propanol | Thermococcus sibiricus | benzaldehyde + NADPH + H+ | - |
? | |
2-pentanol + NADP+ | 67% relative activity as compared to 2-propanol | Thermococcus sibiricus | 2-pentanone + NADPH + H+ | - |
? | |
2-pentanol + NADP+ | 67% of the activity with 2-propanol | Thermococcus sibiricus | pentan-2-one + NADPH + H+ | - |
? | |
2-propanol + NADP+ | - |
Thermococcus sibiricus | acetone + NADPH | - |
? | |
2-propanol + NADP+ | - |
Thermococcus sibiricus | acetone + NADPH + H+ | - |
? | |
3-methyl-2-pentanone + NADPH + H+ | 13% relative activity as compared to pyruvaldehyde | Thermococcus sibiricus | ? + NADP+ | - |
? | |
benzyl alcohol + NADP+ | 180% of the activity with 2-propanol | Thermococcus sibiricus | benzaldehyde + NADPH + H+ | - |
? | |
cellobiose + NADP+ | 71% relative activity as compared to 2-propanol | Thermococcus sibiricus | ? + NADPH + H+ | - |
? | |
cellobiose + NADP+ | 71% of the activity with 2-propanol | Thermococcus sibiricus | ? + NADPH + H+ | - |
? | |
D-arabinose + NADP+ | 200% relative activity as compared to 2-propanol | Thermococcus sibiricus | ? + NADPH + H+ | - |
? | |
D-arabinose + NADP+ | 200% of the activity with 2-propanol. D-arabinose is preferred over L-arabinose | Thermococcus sibiricus | D-arabitol + NADPH + H+ | - |
? | |
D-glucose + NADP+ | 146% relative activity as compared to 2-propanol | Thermococcus sibiricus | ? + NADPH + H+ | - |
? | |
D-glucose + NADP+ | 146% of the activity with 2-propanol | Thermococcus sibiricus | ? + NADPH + H+ | - |
? | |
D-mannose + NADP+ | 48% relative activity as compared to 2-propanol | Thermococcus sibiricus | ? + NADPH + H+ | - |
? | |
D-ribose + NADP+ | 35% relative activity as compared to 2-propanol | Thermococcus sibiricus | ? + NADPH + H+ | - |
? | |
D-xylose + NADP+ | 246% relative activity as compared to 2-propanol | Thermococcus sibiricus | ? + NADPH + H+ | - |
? | |
D-xylose + NADP+ | 246% of the activity with 2-propanol | Thermococcus sibiricus | ? + NADPH + H+ | - |
? | |
dimethylglyoxal + NADPH + H+ | 270% relative activity as compared to pyruvaldehyde | Thermococcus sibiricus | ? + NADP+ | - |
? | |
dimethylglyoxal + NADPH + H+ | 270% of the activity with pyruvaldehyde | Thermococcus sibiricus | ? + NADP+ | - |
? | |
ethanol + NADP+ | 36% relative activity as compared to 2-propanol | Thermococcus sibiricus | acetaldehyde + NADPH + H+ | - |
? | |
glycerol + NADP+ | 16% relative activity as compared to 2-propanol | Thermococcus sibiricus | glyceraldehyde + NADPH + H+ | - |
? | |
glyoxylic acid + NADPH + H+ | 36% relative activity as compared to pyruvaldehyde | Thermococcus sibiricus | ? + NADP+ | - |
? | |
L-arabinose + NADP+ | 17% relative activity as compared to 2-propanol | Thermococcus sibiricus | ? + NADPH + H+ | - |
? | |
additional information | no oxidation of methanol, methoxyethanol, ethylene glycol, or D-galactose. No reduction of acetone, cyclopentanone, D-arabinose, D-xylose, D-glucose or cellobiose | Thermococcus sibiricus | ? | - |
? | |
pyruvaldehyde + NADPH + H+ | - |
Thermococcus sibiricus | lactaldehyde + NADP+ | - |
? | |
pyruvaldehyde + NADPH + H+ | - |
Thermococcus sibiricus | ? + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 26200, calculated from sequence | Thermococcus sibiricus |
dimer | 2 * 28700, SDS-PAGE | Thermococcus sibiricus |
Synonyms | Comment | Organism |
---|---|---|
Tsib_0319 | locus name | Thermococcus sibiricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
assay at | Thermococcus sibiricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
2 h, 10% loss of activity | Thermococcus sibiricus |
70 | - |
1.5 h, no loss of activity | Thermococcus sibiricus |
80 | - |
2 h, 15% loss of activity | Thermococcus sibiricus |
80 | - |
1.5 h, no loss of activity | Thermococcus sibiricus |
90 | - |
half-life: 2 h | Thermococcus sibiricus |
90 | - |
half-life 2 h | Thermococcus sibiricus |
100 | - |
half-life: 1 h | Thermococcus sibiricus |
100 | - |
half-life 1 h | Thermococcus sibiricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.45 | - |
NADP+ | 60°C, pH 10.5 | Thermococcus sibiricus | |
0.45 | - |
NADP+ | pH 10.5, 60°C | Thermococcus sibiricus | |
0.53 | - |
2-propanol | 60°C, pH 10.5 | Thermococcus sibiricus | |
0.53 | - |
2-propanol | pH 10.5, 60°C | Thermococcus sibiricus | |
0.7 | - |
D-xylose | 60°C, pH 10.5 | Thermococcus sibiricus | |
0.7 | - |
D-xylose | pH 10.5, 60°C | Thermococcus sibiricus | |
1.51 | - |
NADPH | pH 7.5, 60°C | Thermococcus sibiricus | |
1.51 | - |
NADPH | 60°C, pH 7.5 | Thermococcus sibiricus | |
2.04 | - |
Pyruvaldehyde | pH 7.5, 60°C | Thermococcus sibiricus | |
2.04 | - |
Pyruvaldehyde | 60°C, pH 7.5 | Thermococcus sibiricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at, reduction of ketones | Thermococcus sibiricus |
10.5 | - |
assay at, oxidation of alcohols | Thermococcus sibiricus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Thermococcus sibiricus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
2-propanol | 60°C, pH 10.5 | Thermococcus sibiricus | |
0.013 | - |
D-xylose | 60°C, pH 10.5 | Thermococcus sibiricus | |
0.11 | - |
Pyruvaldehyde | 60°C, pH 7.5 | Thermococcus sibiricus | |
20.45 | - |
NADP+ | 60°C, pH 10.5 | Thermococcus sibiricus | |
75.5 | - |
NADPH | 60°C, pH 7.5 | Thermococcus sibiricus |