Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli M15[pREP4] cells | Pectobacterium atrosepticum |
Protein Variants | Comment | Organism |
---|---|---|
D194A | the mutation results in substantial catalytic deficiency (2.8% compared to the wild type enzyme) | Pectobacterium atrosepticum |
H198A | inactive | Pectobacterium atrosepticum |
H267A | inactive | Pectobacterium atrosepticum |
H281A | inactive | Pectobacterium atrosepticum |
H363A | the mutant with full activity is not able to grow on butanal-containing medium | Pectobacterium atrosepticum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | complete inhibition at 1 mM | Pectobacterium atrosepticum | |
Fe2+ | no activity at 1 mM | Pectobacterium atrosepticum | |
Ni2+ | 14% residual activity at 1 mM | Pectobacterium atrosepticum | |
Zn2+ | 14% residual activity at 1 mM | Pectobacterium atrosepticum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | required | Pectobacterium atrosepticum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42100 | - |
- |
Pectobacterium atrosepticum |
43200 | - |
- |
Pectobacterium atrosepticum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pectobacterium atrosepticum | U6CL97 | - |
- |
Pectobacterium atrosepticum DSM18077 | U6CL97 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex 200 pg gel filtration | Pectobacterium atrosepticum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
butanal + NADPH + H+ | 44% activity compared to pentanal | Pectobacterium atrosepticum | 1-butanol + NAD(P)+ | - |
? | |
furfural + NADPH + H+ | 5% activity compared to pentanal | Pectobacterium atrosepticum | (furan-2-yl)methanol + NAD(P)+ | - |
? | |
glycoaldehyde + NADPH + H+ | 5% activity compared to pentanal | Pectobacterium atrosepticum | ? | - |
? | |
heptanal + NADPH + H+ | 56% activity compared to pentanal | Pectobacterium atrosepticum | 1-heptanol + NAD(P)+ | - |
? | |
hexanal + NADPH + H+ | 62% activity compared to pentanal | Pectobacterium atrosepticum | 1-hexanol + NAD(P)+ | - |
? | |
methylglyoxal + NADPH + H+ | 29% activity compared to pentanal | Pectobacterium atrosepticum | ? | - |
? | |
additional information | no activity with glyoxal, methanal, and ethanal | Pectobacterium atrosepticum | ? | - |
? | |
additional information | the enzyme shows no oxidative activities towards alcoholic compounds | Pectobacterium atrosepticum | ? | - |
? | |
additional information | no activity with glyoxal, methanal, and ethanal | Pectobacterium atrosepticum DSM18077 | ? | - |
? | |
additional information | the enzyme shows no oxidative activities towards alcoholic compounds | Pectobacterium atrosepticum DSM18077 | ? | - |
? | |
additional information | no activity with glyoxal, methanal, and ethanal | Pectobacterium atrosepticum DSM 18077 | ? | - |
? | |
additional information | the enzyme shows no oxidative activities towards alcoholic compounds | Pectobacterium atrosepticum DSM 18077 | ? | - |
? | |
octanal + NADPH + H+ | 55% activity compared to pentanal | Pectobacterium atrosepticum | 1-octanol + NAD(P)+ | - |
? | |
pentanal + NADPH + H+ | 100% activity | Pectobacterium atrosepticum | 1-pentanol + NAD(P)+ | - |
? | |
propanal + NADPH + H+ | 14% activity compared to pentanal | Pectobacterium atrosepticum | 1-propanol + NAD(P)+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42100, calculated from amino acid sequence | Pectobacterium atrosepticum |
? | x * 43200, SDS-PAGE | Pectobacterium atrosepticum |
Synonyms | Comment | Organism |
---|---|---|
ADH | - |
Pectobacterium atrosepticum |
NAD(P)+-dependent alcohol dehydrogenase | - |
Pectobacterium atrosepticum |
YqhD | - |
Pectobacterium atrosepticum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Pectobacterium atrosepticum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 50 | 30 to 60% of activity is observed between 20° and 30°C and 20% activity at 10 and 50°C, respectively | Pectobacterium atrosepticum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 60 | the enzyme is thermally stable when incubated for 30 min between 4 and 40°C. Incubation for 30 min at temperatures between 10 and 30 °C slightly increases the catalytic activity. Recombinant enzyme is completely inactivated at 50 and 60°C | Pectobacterium atrosepticum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Pectobacterium atrosepticum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | the enzyme shows around 50% of activity at pH 6.0, pH 6.5, and pH 8.0 | Pectobacterium atrosepticum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Pectobacterium atrosepticum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pectobacterium atrosepticum | calculated from amino acid sequence | - |
5.5 |