KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | enzyme kinetic parameters are determined by fitting the initial rates to irreversible single substrate Michaelis-Menten models | Lactiplantibacillus plantarum | |
additional information | - |
additional information | enzyme kinetic parameters are determined by fitting the initial rates to irreversible single substrate Michaelis-Menten models | Lactobacillus delbrueckii | |
additional information | - |
additional information | enzyme kinetic parameters are determined by fitting the initial rates to irreversible single substrate Michaelis-Menten models | Lactobacillus acidophilus | |
12.8 | - |
D-glyceraldehyde | reverse reaction, pH 6.5, 25°C, crude extract | Lactobacillus delbrueckii | |
20.2 | - |
D-glyceraldehyde | reverse reaction, pH 6.5, 25°C, crude extract | Lactiplantibacillus plantarum | |
32.3 | - |
D-glyceraldehyde | reverse reaction, pH 6.5, 25°C, crude extract | Lactobacillus acidophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerol + NADP+ | Lactiplantibacillus plantarum | - |
D-glyceraldehyde + NADPH + H+ | - |
r | |
glycerol + NADP+ | Lactobacillus delbrueckii | - |
D-glyceraldehyde + NADPH + H+ | - |
r | |
glycerol + NADP+ | Lactobacillus acidophilus | - |
D-glyceraldehyde + NADPH + H+ | - |
r | |
glycerol + NADP+ | Lactiplantibacillus plantarum ATCC 8014 | - |
D-glyceraldehyde + NADPH + H+ | - |
r | |
glycerol + NADP+ | Lactobacillus delbrueckii UFV-H2B20 | - |
D-glyceraldehyde + NADPH + H+ | - |
r | |
glycerol + NADP+ | Lactobacillus acidophilus ATCC 4356 | - |
D-glyceraldehyde + NADPH + H+ | - |
r | |
additional information | Lactiplantibacillus plantarum | glycerol dehydrogenase activity is detected in all Lactobacillus strains studied but only for the reduction of glyceraldehyde with NADPH. This enzyme shows a very low oxidative activity with glycerol and NADP+ and, most likely, under physiological conditions, the oxidative reaction does not occur | ? | - |
? | |
additional information | Lactobacillus delbrueckii | glycerol dehydrogenase activity is detected in all Lactobacillus strains studied. This enzyme shows a very low oxidative activity with glycerol and NADP+ and, most likely, under physiological conditions, the oxidative reaction does not occur | ? | - |
? | |
additional information | Lactobacillus acidophilus | glycerol dehydrogenase activity is detected in all Lactobacillus strains. This enzyme shows a very low oxidative activity with glycerol and NADP+ and, most likely, under physiological conditions, the oxidative reaction does not occur | ? | - |
? | |
additional information | Lactiplantibacillus plantarum ATCC 8014 | glycerol dehydrogenase activity is detected in all Lactobacillus strains studied but only for the reduction of glyceraldehyde with NADPH. This enzyme shows a very low oxidative activity with glycerol and NADP+ and, most likely, under physiological conditions, the oxidative reaction does not occur | ? | - |
? | |
additional information | Lactobacillus delbrueckii UFV-H2B20 | glycerol dehydrogenase activity is detected in all Lactobacillus strains studied. This enzyme shows a very low oxidative activity with glycerol and NADP+ and, most likely, under physiological conditions, the oxidative reaction does not occur | ? | - |
? | |
additional information | Lactobacillus acidophilus ATCC 4356 | glycerol dehydrogenase activity is detected in all Lactobacillus strains. This enzyme shows a very low oxidative activity with glycerol and NADP+ and, most likely, under physiological conditions, the oxidative reaction does not occur | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactiplantibacillus plantarum | - |
- |
- |
Lactiplantibacillus plantarum ATCC 8014 | - |
- |
- |
Lactobacillus acidophilus | - |
- |
- |
Lactobacillus acidophilus ATCC 4356 | - |
- |
- |
Lactobacillus delbrueckii | - |
- |
- |
Lactobacillus delbrueckii UFV-H2B20 | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | biodiesel-derived glycerol is used as the main carbon and energy source in batch microaerobic growth, the tested strains are able to assimilate glycerol, consuming between 38-48% in approximately 24 h. The strains catabolize glycerol mainly through glycerol kinase, EC 2.7.1.30. Glycerol dehydrogenase activity is also detected in the strains studied but only for the reduction of glyceraldehyde with NADPH | Lactiplantibacillus plantarum | - |
additional information | biodiesel-derived glycerol is used as the main carbon and energy source in batch microaerobic growth, the tested strains are able to assimilate glycerol, consuming between 38-48% in approximately 24 h. The strains catabolize glycerol mainly through glycerol kinase, EC 2.7.1.30. Glycerol dehydrogenase activity is also detected in the strains studied but only for the reduction of glyceraldehyde with NADPH | Lactobacillus delbrueckii | - |
additional information | biodiesel-derived glycerol is used as the main carbon and energy source in batch microaerobic growth, the tested strains are able to assimilate glycerol, consuming between 38-48% in approximately 24 h. The strains catabolize glycerol mainly through glycerol kinase, EC 2.7.1.30. Glycerol dehydrogenase activity is also detected in the strains studied but only for the reduction of glyceraldehyde with NADPH | Lactobacillus acidophilus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0043 | - |
cell-free enzyme extract, reverse reaction, pH 6.5, 25°C | Lactobacillus delbrueckii |
0.0049 | - |
cell-free enzyme extract, reverse reaction, pH 6.5, 25°C | Lactiplantibacillus plantarum |
0.0131 | - |
cell-free enzyme extract, reverse reaction, pH 6.5, 25°C | Lactobacillus acidophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerol + NADP+ | - |
Lactiplantibacillus plantarum | D-glyceraldehyde + NADPH + H+ | - |
r | |
glycerol + NADP+ | - |
Lactobacillus delbrueckii | D-glyceraldehyde + NADPH + H+ | - |
r | |
glycerol + NADP+ | - |
Lactobacillus acidophilus | D-glyceraldehyde + NADPH + H+ | - |
r | |
glycerol + NADP+ | the reverse reaction is energetically more favorable | Lactiplantibacillus plantarum | D-glyceraldehyde + NADPH + H+ | - |
r | |
glycerol + NADP+ | the reverse reaction is energetically more favorable | Lactobacillus delbrueckii | D-glyceraldehyde + NADPH + H+ | - |
r | |
glycerol + NADP+ | the reverse reaction is energetically more favorable | Lactobacillus acidophilus | D-glyceraldehyde + NADPH + H+ | - |
r | |
glycerol + NADP+ | - |
Lactiplantibacillus plantarum ATCC 8014 | D-glyceraldehyde + NADPH + H+ | - |
r | |
glycerol + NADP+ | the reverse reaction is energetically more favorable | Lactiplantibacillus plantarum ATCC 8014 | D-glyceraldehyde + NADPH + H+ | - |
r | |
glycerol + NADP+ | - |
Lactobacillus delbrueckii UFV-H2B20 | D-glyceraldehyde + NADPH + H+ | - |
r | |
glycerol + NADP+ | the reverse reaction is energetically more favorable | Lactobacillus delbrueckii UFV-H2B20 | D-glyceraldehyde + NADPH + H+ | - |
r | |
glycerol + NADP+ | - |
Lactobacillus acidophilus ATCC 4356 | D-glyceraldehyde + NADPH + H+ | - |
r | |
glycerol + NADP+ | the reverse reaction is energetically more favorable | Lactobacillus acidophilus ATCC 4356 | D-glyceraldehyde + NADPH + H+ | - |
r | |
additional information | glycerol dehydrogenase activity is detected in all Lactobacillus strains studied but only for the reduction of glyceraldehyde with NADPH. This enzyme shows a very low oxidative activity with glycerol and NADP+ and, most likely, under physiological conditions, the oxidative reaction does not occur | Lactiplantibacillus plantarum | ? | - |
? | |
additional information | glycerol dehydrogenase activity is detected in all Lactobacillus strains studied. This enzyme shows a very low oxidative activity with glycerol and NADP+ and, most likely, under physiological conditions, the oxidative reaction does not occur | Lactobacillus delbrueckii | ? | - |
? | |
additional information | glycerol dehydrogenase activity is detected in all Lactobacillus strains. This enzyme shows a very low oxidative activity with glycerol and NADP+ and, most likely, under physiological conditions, the oxidative reaction does not occur | Lactobacillus acidophilus | ? | - |
? | |
additional information | glycerol dehydrogenase activity is detected in all Lactobacillus strains studied but only for the reduction of glyceraldehyde with NADPH. This enzyme shows a very low oxidative activity with glycerol and NADP+ and, most likely, under physiological conditions, the oxidative reaction does not occur | Lactiplantibacillus plantarum ATCC 8014 | ? | - |
? | |
additional information | glycerol dehydrogenase activity is detected in all Lactobacillus strains studied. This enzyme shows a very low oxidative activity with glycerol and NADP+ and, most likely, under physiological conditions, the oxidative reaction does not occur | Lactobacillus delbrueckii UFV-H2B20 | ? | - |
? | |
additional information | glycerol dehydrogenase activity is detected in all Lactobacillus strains. This enzyme shows a very low oxidative activity with glycerol and NADP+ and, most likely, under physiological conditions, the oxidative reaction does not occur | Lactobacillus acidophilus ATCC 4356 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glycerol dehydrogenase | - |
Lactiplantibacillus plantarum |
glycerol dehydrogenase | - |
Lactobacillus delbrueckii |
glycerol dehydrogenase | - |
Lactobacillus acidophilus |
NADP+-dependent glycerol dehydrogenase | - |
Lactiplantibacillus plantarum |
NADP+-dependent glycerol dehydrogenase | - |
Lactobacillus delbrueckii |
NADP+-dependent glycerol dehydrogenase | - |
Lactobacillus acidophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at, forward reaction | Lactiplantibacillus plantarum |
25 | - |
assay at, forward reaction | Lactobacillus delbrueckii |
25 | - |
assay at, forward reaction | Lactobacillus acidophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at, reverse reaction | Lactiplantibacillus plantarum |
6.5 | - |
assay at, reverse reaction | Lactobacillus delbrueckii |
6.5 | - |
assay at, reverse reaction | Lactobacillus acidophilus |
10 | - |
assay at, forward reaction | Lactiplantibacillus plantarum |
10 | - |
assay at, forward reaction | Lactobacillus delbrueckii |
10 | - |
assay at, forward reaction | Lactobacillus acidophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Lactiplantibacillus plantarum | |
NADP+ | - |
Lactobacillus delbrueckii | |
NADP+ | - |
Lactobacillus acidophilus | |
NADPH | - |
Lactiplantibacillus plantarum | |
NADPH | - |
Lactobacillus delbrueckii | |
NADPH | - |
Lactobacillus acidophilus |
General Information | Comment | Organism |
---|---|---|
evolution | glycerol dehydrogenases can be classified into three different groups. The first one, NAD+-dependent (EC 1.1.1.6), catalyzes the oxidation of glycerol to DHA. The second (EC 1.1.1.156), also named DHA reductase, oxidizes glycerol to DHA in the presence of nicotinamide adenine dinucleotide phosphate (NADP+). The third one (EC 1.1.1.72) transforms glycerol to D-glyceraldehyde in the presence of NADP+. The latter enzymes also use D-glyceraldehyde, or its stereoisomer, as substrate in a reverse reaction for producing glycerol, as in glycerolipid metabolism | Lactiplantibacillus plantarum |
evolution | glycerol dehydrogenases can be classified into three different groups. The first one, NAD+-dependent (EC 1.1.1.6), catalyzes the oxidation of glycerol to DHA. The second (EC 1.1.1.156), also named DHA reductase, oxidizes glycerol to DHA in the presence of nicotinamide adenine dinucleotide phosphate (NADP+). The third one (EC 1.1.1.72) transforms glycerol to D-glyceraldehyde in the presence of NADP+. The latter enzymes also use D-glyceraldehyde, or its stereoisomer, as substrate in a reverse reaction for producing glycerol, as in glycerolipid metabolism | Lactobacillus delbrueckii |
evolution | glycerol dehydrogenases can be classified into three different groups. The first one, NAD+-dependent (EC 1.1.1.6), catalyzes the oxidation of glycerol to DHA. The second (EC 1.1.1.156), also named DHA reductase, oxidizes glycerol to DHA in the presence of nicotinamide adenine dinucleotide phosphate (NADP+). The third one (EC 1.1.1.72) transforms glycerol to D-glyceraldehyde in the presence of NADP+. The latter enzymes also use D-glyceraldehyde, or its stereoisomer, as substrate in a reverse reaction for producing glycerol, as in glycerolipid metabolism | Lactobacillus acidophilus |
metabolism | glycerol dehydrogenase activity is detected in all Lactobacillus strains studied but only for the reduction of glyceraldehyde with NADPH (Km for DL-glyceraldehyde ranging from 12.8 to 32.3 mM). This enzyme shows a very low oxidative activity with glycerol and NADP+ and, most likely, under physiological conditions, the oxidative reaction does not occur, supporting the assumption that the main metabolic flux concerning glycerol metabolism is through the glycerol kinase pathway, but glycerol kinase is not completely controlling the flux of glycerol metabolism in the lactobacilli | Lactobacillus delbrueckii |
metabolism | glycerol dehydrogenase activity is detected in all Lactobacillus strains studied but only for the reduction of glyceraldehyde with NADPH (Km for DL-glyceraldehyde ranging from 12.8 to 32.3 mM). This enzyme shows a very low oxidative activity with glycerol and NADP+ and, most likely, under physiological conditions, the oxidative reaction does not occur, supporting the assumption that the main metabolic flux concerning glycerol metabolism is through the glycerol kinase pathway, but glycerol kinase is not completely controlling the flux of glycerol metabolism in the lactobacilli | Lactobacillus acidophilus |
metabolism | glycerol dehydrogenase activity is detected in all Lactobacillus strains studied. This enzyme shows a very low oxidative activity with glycerol and NADP+ and, most likely, under physiological conditions, the oxidative reaction does not occur, supporting the assumption that the main metabolic flux concerning glycerol metabolism is through the glycerol kinase pathway, but glycerol kinase is not completely controlling the flux of glycerol metabolism in the lactobacilli | Lactiplantibacillus plantarum |