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Literature summary for 1.1.1.72 extracted from

  • Rivaldi, J.; Sousa Silva, M.; Duarte, L.; Ferreira, A.; Cordeiro, C.; De Almeida Felipe, M.; De Ponces Freire, A.; De Mancilha, I.
    Metabolism of biodiesel-derived glycerol in probiotic Lactobacillus strains (2013), Appl. Microbiol. Biotechnol., 97, 1735-1743.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information enzyme kinetic parameters are determined by fitting the initial rates to irreversible single substrate Michaelis-Menten models Lactiplantibacillus plantarum
additional information
-
additional information enzyme kinetic parameters are determined by fitting the initial rates to irreversible single substrate Michaelis-Menten models Lactobacillus delbrueckii
additional information
-
additional information enzyme kinetic parameters are determined by fitting the initial rates to irreversible single substrate Michaelis-Menten models Lactobacillus acidophilus
12.8
-
D-glyceraldehyde reverse reaction, pH 6.5, 25°C, crude extract Lactobacillus delbrueckii
20.2
-
D-glyceraldehyde reverse reaction, pH 6.5, 25°C, crude extract Lactiplantibacillus plantarum
32.3
-
D-glyceraldehyde reverse reaction, pH 6.5, 25°C, crude extract Lactobacillus acidophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycerol + NADP+ Lactiplantibacillus plantarum
-
D-glyceraldehyde + NADPH + H+
-
r
glycerol + NADP+ Lactobacillus delbrueckii
-
D-glyceraldehyde + NADPH + H+
-
r
glycerol + NADP+ Lactobacillus acidophilus
-
D-glyceraldehyde + NADPH + H+
-
r
glycerol + NADP+ Lactiplantibacillus plantarum ATCC 8014
-
D-glyceraldehyde + NADPH + H+
-
r
glycerol + NADP+ Lactobacillus delbrueckii UFV-H2B20
-
D-glyceraldehyde + NADPH + H+
-
r
glycerol + NADP+ Lactobacillus acidophilus ATCC 4356
-
D-glyceraldehyde + NADPH + H+
-
r
additional information Lactiplantibacillus plantarum glycerol dehydrogenase activity is detected in all Lactobacillus strains studied but only for the reduction of glyceraldehyde with NADPH. This enzyme shows a very low oxidative activity with glycerol and NADP+ and, most likely, under physiological conditions, the oxidative reaction does not occur ?
-
?
additional information Lactobacillus delbrueckii glycerol dehydrogenase activity is detected in all Lactobacillus strains studied. This enzyme shows a very low oxidative activity with glycerol and NADP+ and, most likely, under physiological conditions, the oxidative reaction does not occur ?
-
?
additional information Lactobacillus acidophilus glycerol dehydrogenase activity is detected in all Lactobacillus strains. This enzyme shows a very low oxidative activity with glycerol and NADP+ and, most likely, under physiological conditions, the oxidative reaction does not occur ?
-
?
additional information Lactiplantibacillus plantarum ATCC 8014 glycerol dehydrogenase activity is detected in all Lactobacillus strains studied but only for the reduction of glyceraldehyde with NADPH. This enzyme shows a very low oxidative activity with glycerol and NADP+ and, most likely, under physiological conditions, the oxidative reaction does not occur ?
-
?
additional information Lactobacillus delbrueckii UFV-H2B20 glycerol dehydrogenase activity is detected in all Lactobacillus strains studied. This enzyme shows a very low oxidative activity with glycerol and NADP+ and, most likely, under physiological conditions, the oxidative reaction does not occur ?
-
?
additional information Lactobacillus acidophilus ATCC 4356 glycerol dehydrogenase activity is detected in all Lactobacillus strains. This enzyme shows a very low oxidative activity with glycerol and NADP+ and, most likely, under physiological conditions, the oxidative reaction does not occur ?
-
?

Organism

Organism UniProt Comment Textmining
Lactiplantibacillus plantarum
-
-
-
Lactiplantibacillus plantarum ATCC 8014
-
-
-
Lactobacillus acidophilus
-
-
-
Lactobacillus acidophilus ATCC 4356
-
-
-
Lactobacillus delbrueckii
-
-
-
Lactobacillus delbrueckii UFV-H2B20
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information biodiesel-derived glycerol is used as the main carbon and energy source in batch microaerobic growth, the tested strains are able to assimilate glycerol, consuming between 38-48% in approximately 24 h. The strains catabolize glycerol mainly through glycerol kinase, EC 2.7.1.30. Glycerol dehydrogenase activity is also detected in the strains studied but only for the reduction of glyceraldehyde with NADPH Lactiplantibacillus plantarum
-
additional information biodiesel-derived glycerol is used as the main carbon and energy source in batch microaerobic growth, the tested strains are able to assimilate glycerol, consuming between 38-48% in approximately 24 h. The strains catabolize glycerol mainly through glycerol kinase, EC 2.7.1.30. Glycerol dehydrogenase activity is also detected in the strains studied but only for the reduction of glyceraldehyde with NADPH Lactobacillus delbrueckii
-
additional information biodiesel-derived glycerol is used as the main carbon and energy source in batch microaerobic growth, the tested strains are able to assimilate glycerol, consuming between 38-48% in approximately 24 h. The strains catabolize glycerol mainly through glycerol kinase, EC 2.7.1.30. Glycerol dehydrogenase activity is also detected in the strains studied but only for the reduction of glyceraldehyde with NADPH Lactobacillus acidophilus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0043
-
cell-free enzyme extract, reverse reaction, pH 6.5, 25°C Lactobacillus delbrueckii
0.0049
-
cell-free enzyme extract, reverse reaction, pH 6.5, 25°C Lactiplantibacillus plantarum
0.0131
-
cell-free enzyme extract, reverse reaction, pH 6.5, 25°C Lactobacillus acidophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycerol + NADP+
-
Lactiplantibacillus plantarum D-glyceraldehyde + NADPH + H+
-
r
glycerol + NADP+
-
Lactobacillus delbrueckii D-glyceraldehyde + NADPH + H+
-
r
glycerol + NADP+
-
Lactobacillus acidophilus D-glyceraldehyde + NADPH + H+
-
r
glycerol + NADP+ the reverse reaction is energetically more favorable Lactiplantibacillus plantarum D-glyceraldehyde + NADPH + H+
-
r
glycerol + NADP+ the reverse reaction is energetically more favorable Lactobacillus delbrueckii D-glyceraldehyde + NADPH + H+
-
r
glycerol + NADP+ the reverse reaction is energetically more favorable Lactobacillus acidophilus D-glyceraldehyde + NADPH + H+
-
r
glycerol + NADP+
-
Lactiplantibacillus plantarum ATCC 8014 D-glyceraldehyde + NADPH + H+
-
r
glycerol + NADP+ the reverse reaction is energetically more favorable Lactiplantibacillus plantarum ATCC 8014 D-glyceraldehyde + NADPH + H+
-
r
glycerol + NADP+
-
Lactobacillus delbrueckii UFV-H2B20 D-glyceraldehyde + NADPH + H+
-
r
glycerol + NADP+ the reverse reaction is energetically more favorable Lactobacillus delbrueckii UFV-H2B20 D-glyceraldehyde + NADPH + H+
-
r
glycerol + NADP+
-
Lactobacillus acidophilus ATCC 4356 D-glyceraldehyde + NADPH + H+
-
r
glycerol + NADP+ the reverse reaction is energetically more favorable Lactobacillus acidophilus ATCC 4356 D-glyceraldehyde + NADPH + H+
-
r
additional information glycerol dehydrogenase activity is detected in all Lactobacillus strains studied but only for the reduction of glyceraldehyde with NADPH. This enzyme shows a very low oxidative activity with glycerol and NADP+ and, most likely, under physiological conditions, the oxidative reaction does not occur Lactiplantibacillus plantarum ?
-
?
additional information glycerol dehydrogenase activity is detected in all Lactobacillus strains studied. This enzyme shows a very low oxidative activity with glycerol and NADP+ and, most likely, under physiological conditions, the oxidative reaction does not occur Lactobacillus delbrueckii ?
-
?
additional information glycerol dehydrogenase activity is detected in all Lactobacillus strains. This enzyme shows a very low oxidative activity with glycerol and NADP+ and, most likely, under physiological conditions, the oxidative reaction does not occur Lactobacillus acidophilus ?
-
?
additional information glycerol dehydrogenase activity is detected in all Lactobacillus strains studied but only for the reduction of glyceraldehyde with NADPH. This enzyme shows a very low oxidative activity with glycerol and NADP+ and, most likely, under physiological conditions, the oxidative reaction does not occur Lactiplantibacillus plantarum ATCC 8014 ?
-
?
additional information glycerol dehydrogenase activity is detected in all Lactobacillus strains studied. This enzyme shows a very low oxidative activity with glycerol and NADP+ and, most likely, under physiological conditions, the oxidative reaction does not occur Lactobacillus delbrueckii UFV-H2B20 ?
-
?
additional information glycerol dehydrogenase activity is detected in all Lactobacillus strains. This enzyme shows a very low oxidative activity with glycerol and NADP+ and, most likely, under physiological conditions, the oxidative reaction does not occur Lactobacillus acidophilus ATCC 4356 ?
-
?

Synonyms

Synonyms Comment Organism
glycerol dehydrogenase
-
Lactiplantibacillus plantarum
glycerol dehydrogenase
-
Lactobacillus delbrueckii
glycerol dehydrogenase
-
Lactobacillus acidophilus
NADP+-dependent glycerol dehydrogenase
-
Lactiplantibacillus plantarum
NADP+-dependent glycerol dehydrogenase
-
Lactobacillus delbrueckii
NADP+-dependent glycerol dehydrogenase
-
Lactobacillus acidophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at, forward reaction Lactiplantibacillus plantarum
25
-
assay at, forward reaction Lactobacillus delbrueckii
25
-
assay at, forward reaction Lactobacillus acidophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
assay at, reverse reaction Lactiplantibacillus plantarum
6.5
-
assay at, reverse reaction Lactobacillus delbrueckii
6.5
-
assay at, reverse reaction Lactobacillus acidophilus
10
-
assay at, forward reaction Lactiplantibacillus plantarum
10
-
assay at, forward reaction Lactobacillus delbrueckii
10
-
assay at, forward reaction Lactobacillus acidophilus

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Lactiplantibacillus plantarum
NADP+
-
Lactobacillus delbrueckii
NADP+
-
Lactobacillus acidophilus
NADPH
-
Lactiplantibacillus plantarum
NADPH
-
Lactobacillus delbrueckii
NADPH
-
Lactobacillus acidophilus

General Information

General Information Comment Organism
evolution glycerol dehydrogenases can be classified into three different groups. The first one, NAD+-dependent (EC 1.1.1.6), catalyzes the oxidation of glycerol to DHA. The second (EC 1.1.1.156), also named DHA reductase, oxidizes glycerol to DHA in the presence of nicotinamide adenine dinucleotide phosphate (NADP+). The third one (EC 1.1.1.72) transforms glycerol to D-glyceraldehyde in the presence of NADP+. The latter enzymes also use D-glyceraldehyde, or its stereoisomer, as substrate in a reverse reaction for producing glycerol, as in glycerolipid metabolism Lactiplantibacillus plantarum
evolution glycerol dehydrogenases can be classified into three different groups. The first one, NAD+-dependent (EC 1.1.1.6), catalyzes the oxidation of glycerol to DHA. The second (EC 1.1.1.156), also named DHA reductase, oxidizes glycerol to DHA in the presence of nicotinamide adenine dinucleotide phosphate (NADP+). The third one (EC 1.1.1.72) transforms glycerol to D-glyceraldehyde in the presence of NADP+. The latter enzymes also use D-glyceraldehyde, or its stereoisomer, as substrate in a reverse reaction for producing glycerol, as in glycerolipid metabolism Lactobacillus delbrueckii
evolution glycerol dehydrogenases can be classified into three different groups. The first one, NAD+-dependent (EC 1.1.1.6), catalyzes the oxidation of glycerol to DHA. The second (EC 1.1.1.156), also named DHA reductase, oxidizes glycerol to DHA in the presence of nicotinamide adenine dinucleotide phosphate (NADP+). The third one (EC 1.1.1.72) transforms glycerol to D-glyceraldehyde in the presence of NADP+. The latter enzymes also use D-glyceraldehyde, or its stereoisomer, as substrate in a reverse reaction for producing glycerol, as in glycerolipid metabolism Lactobacillus acidophilus
metabolism glycerol dehydrogenase activity is detected in all Lactobacillus strains studied but only for the reduction of glyceraldehyde with NADPH (Km for DL-glyceraldehyde ranging from 12.8 to 32.3 mM). This enzyme shows a very low oxidative activity with glycerol and NADP+ and, most likely, under physiological conditions, the oxidative reaction does not occur, supporting the assumption that the main metabolic flux concerning glycerol metabolism is through the glycerol kinase pathway, but glycerol kinase is not completely controlling the flux of glycerol metabolism in the lactobacilli Lactobacillus delbrueckii
metabolism glycerol dehydrogenase activity is detected in all Lactobacillus strains studied but only for the reduction of glyceraldehyde with NADPH (Km for DL-glyceraldehyde ranging from 12.8 to 32.3 mM). This enzyme shows a very low oxidative activity with glycerol and NADP+ and, most likely, under physiological conditions, the oxidative reaction does not occur, supporting the assumption that the main metabolic flux concerning glycerol metabolism is through the glycerol kinase pathway, but glycerol kinase is not completely controlling the flux of glycerol metabolism in the lactobacilli Lactobacillus acidophilus
metabolism glycerol dehydrogenase activity is detected in all Lactobacillus strains studied. This enzyme shows a very low oxidative activity with glycerol and NADP+ and, most likely, under physiological conditions, the oxidative reaction does not occur, supporting the assumption that the main metabolic flux concerning glycerol metabolism is through the glycerol kinase pathway, but glycerol kinase is not completely controlling the flux of glycerol metabolism in the lactobacilli Lactiplantibacillus plantarum