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Literature summary for 1.1.1.76 extracted from
- Laboratory-scale production of acetoin isomers (D(-) and L(+)) by bacterial fermentation (1984), J. Ferment. Technol., 62, 151-156.
No PubMed abstract available
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | increased acetoin production | Corynebacterium glutamicum |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Corynebacterium glutamicum | - |
C-1012 | - |
| Corynebacterium glutamicum C-1012 | - |
C-1012 | - |
| Klebsiella pneumoniae | - |
IAM 1063 | - |
| Klebsiella pneumoniae IAM 1063 | - |
IAM 1063 | - |
| no activity in Pseudomonas sp. | - |
lacks 2,3-butanediol dehydrogenase | - |
| no activity in Pseudomonas sp. s4 | - |
lacks 2,3-butanediol dehydrogenase | - |
| Paenibacillus polymyxa | - |
IAM 1189 | - |
| Paenibacillus polymyxa IAM 1189 | - |
IAM 1189 | - |
| Saccharomyces cerevisiae | - |
OC-2 | - |
| Saccharomyces cerevisiae OC-2 | - |
OC-2 | - |
| Serratia marcescens | - |
IAM 1022 | - |
| Serratia marcescens IAM 1022 | - |
IAM 1022 | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.44 | - |
3% 2,3-butanediol as carbon source | Corynebacterium glutamicum |
| 0.79 | - |
1% glucose as carbon source | Corynebacterium glutamicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-acetoin + NADH | - |
Klebsiella pneumoniae | L-2,3-butanediol | - |
r | |
| L-acetoin + NADH | reaction dependent of substrate concentration, incubation time, glucose addition, aeration | Corynebacterium glutamicum | L-2,3-butanediol | - |
r | |
| L-acetoin + NADH | reaction dependent of substrate concentration, incubation time, glucose addition, aeration | Corynebacterium glutamicum C-1012 | L-2,3-butanediol | - |
r | |
| L-acetoin + NADH | - |
Klebsiella pneumoniae IAM 1063 | L-2,3-butanediol | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Corynebacterium glutamicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | - |
assay at, reduction of acetoin | Corynebacterium glutamicum |
| 8 | - |
assay at, oxidation of 2,3-butanediol | Corynebacterium glutamicum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Klebsiella pneumoniae | |
| NAD+ | - |
Corynebacterium glutamicum | |
| NADH | - |
Klebsiella pneumoniae | |
| NADH | - |
Corynebacterium glutamicum | |
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