Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | slight activation | Escherichia coli | |
dithiothreitol | slight activation | Escherichia coli | |
glutathione | slight activation | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Escherichia coli | |
Cu2+ | - |
Escherichia coli | |
Hg2+ | - |
Escherichia coli | |
iodoacetate | - |
Escherichia coli | |
N-ethylmaleimide | - |
Escherichia coli | |
Ni2+ | - |
Escherichia coli | |
p-chloromercuribenzoate | - |
Escherichia coli | |
Zn2+ | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
gel filtration, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methylglyoxal + NADH | Escherichia coli | - |
(R)-lactaldehyde + NAD+ | - |
? | |
methylglyoxal + NADPH | Escherichia coli | - |
lactaldehyde + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.26 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methylglyoxal + NADH | - |
Escherichia coli | (R)-lactaldehyde + NAD+ | - |
? | |
methylglyoxal + NADPH | - |
Escherichia coli | lactaldehyde + NADP+ | - |
? | |
additional information | enzyme utilizes both: NADH and NADPH | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 43000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Escherichia coli |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 75 | 25°C and 75°C: about 50% of activity maximum | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 9.5 | pH 4.5 and pH 9.5: about 50% of activity maximum | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | utilizes both NADPH and NADH | Escherichia coli | |
NADPH | utilizes both NADH and NADPH | Escherichia coli |