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Literature summary for 1.1.1.79 extracted from
- Kinetic mechanism of a recombinant Arabidopsis glyoxylate reductase: studies of initial velocity, dead-end inhibition and product inhibition (2007), Can. J. Bot., 85, 896-902.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-hydroxybutyrate | mixed type inhibition with NADPH; mixed type inhibition with succinic semialdehyde | Arabidopsis thaliana |  |
| glycolate | mixed type inhibition with glyoxylate; uncompetitive with NADPH | Arabidopsis thaliana | |
| NADP+ | competitive with NADPH; competitive with NADPH when glyoxylate is the fixed substrate; uncompetitive with glyoxylate; uncompetitive with succinic semialdehyde | Arabidopsis thaliana | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0045 | - |
glyoxylate | recombinant protein from Escherichia coli | Arabidopsis thaliana | |
| 0.87 | - |
Succinic semialdehyde | recombinant protein from Escherichia coli | Arabidopsis thaliana | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q9LSV0 | enzyme also displays 4-hydroxybutyrate dehydrogenase (EC1.1.1.61) activity but with less efficiency; L. Heynh | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein from Escherichia coli | Arabidopsis thaliana |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| glycolate + NADP+ = glyoxylate + NADPH + H+ | ordered Bi Bi mechanism, complexation of NADPH to the enzyme before glyoxylate or succinic semialdehyde, and release of NADP+ before glycolate or 4-hydroxybutyrate | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glyoxylate + NADPH + H+ | preferred substrate | Arabidopsis thaliana | glycolate + NADP+ | - |
ir | |
| succinic semialdehyde + NADPH + H+ | - |
Arabidopsis thaliana | 4-hydroxybutyrate + NADP+ | - |
ir | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AtGR1 | - |
Arabidopsis thaliana |
| More | enzyme also displays 4-hydroxybutyrate dehydrogenase (EC1.1.1.61) activity but with less efficiency | Arabidopsis thaliana |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Arabidopsis thaliana | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0031 | - |
NADP+ | competitive with NADPH | Arabidopsis thaliana | |
| 0.0095 | - |
NADP+ | competitive with NADPH when glyoxylate is the fixed substrate | Arabidopsis thaliana | |
| 0.125 | - |
NADP+ | uncompetitive with succinic semialdehyde | Arabidopsis thaliana | |
| 0.147 | - |
NADP+ | uncompetitive with glyoxylate | Arabidopsis thaliana | |
| 22.1 | - |
glycolate | uncompetitive with NADPH | Arabidopsis thaliana | |
| 23.7 | - |
glycolate | mixed type inhibition with glyoxylate | Arabidopsis thaliana | |
| 79.9 | - |
4-hydroxybutyrate | mixed type inhibition with NADPH | Arabidopsis thaliana | |
| 93.5 | - |
4-hydroxybutyrate | mixed type inhibition with succinic semialdehyde | Arabidopsis thaliana | |
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