Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Acetobacter aceti |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ethanol | enzyme activity is decreased to 20% by incubation with 60% (v/v) ethanol | Acetobacter aceti | |
Fe3+ | 15% inhibition at 1 mM | Acetobacter aceti | |
Hg2+ | 30% inhibition at 1 mM | Acetobacter aceti |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.38 | - |
glyoxylate | with NADPH as cosubstrate, at pH 4.0 and 45°C | Acetobacter aceti | |
0.58 | - |
glyoxylate | with NADH as cosubstrate, at pH 4.0 and 45°C | Acetobacter aceti | |
309 | - |
glycolate | with NAD+ as cosubstrate, at pH 9.0 and 45°C | Acetobacter aceti | |
334 | - |
glycolate | with NADP+ as cosubstrate, at pH 9.0 and 45°C | Acetobacter aceti |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxylate + NADH + H+ | Acetobacter aceti | the specific activity with NADPH is slightly higher as that with NADH | glycolate + NAD+ | - |
? | |
glyoxylate + NADH + H+ | Acetobacter aceti JCM20276 | the specific activity with NADPH is slightly higher as that with NADH | glycolate + NAD+ | - |
? | |
glyoxylate + NADPH + H+ | Acetobacter aceti | the specific activity with NADPH is slightly higher as that with NADH | glycolate + NADP+ | - |
? | |
glyoxylate + NADPH + H+ | Acetobacter aceti JCM20276 | the specific activity with NADPH is slightly higher as that with NADH | glycolate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetobacter aceti | - |
- |
- |
Acetobacter aceti JCM20276 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Acetobacter aceti |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
463 | - |
crude extract, the reaction mixture contains 100 mM citrate buffer (pH 4.0), 1 mM glyoxylate, and 0.15 mM NADPH at 45°C | Acetobacter aceti |
1300 | - |
after 2.81fold purification, the reaction mixture contains 100 mM citrate buffer (pH 4.0), 1 mM glyoxylate, and 0.15 mM NADPH at 45°C | Acetobacter aceti |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycolate + NAD+ | the reaction occurs only at pH 9.0 | Acetobacter aceti | glyoxylate + NADH + H+ | - |
? | |
glycolate + NAD+ | the reaction occurs only at pH 9.0 | Acetobacter aceti JCM20276 | glyoxylate + NADH + H+ | - |
? | |
glycolate + NADP+ | the reaction occurs only at pH 9.0 | Acetobacter aceti | glyoxylate + NAPDH + H+ | - |
? | |
glycolate + NADP+ | the reaction occurs only at pH 9.0 | Acetobacter aceti JCM20276 | glyoxylate + NAPDH + H+ | - |
? | |
glyoxylate + NADH + H+ | the specific activity with NADPH is slightly higher as that with NADH | Acetobacter aceti | glycolate + NAD+ | - |
? | |
glyoxylate + NADH + H+ | the specific activity with NADPH is slightly higher as that with NADH | Acetobacter aceti JCM20276 | glycolate + NAD+ | - |
? | |
glyoxylate + NADPH + H+ | the specific activity with NADPH is slightly higher as that with NADH | Acetobacter aceti | glycolate + NADP+ | - |
? | |
glyoxylate + NADPH + H+ | the specific activity with NADPH is slightly higher as that with NADH | Acetobacter aceti JCM20276 | glycolate + NADP+ | - |
? | |
additional information | the enzyme exhibits no activity against succinic semialdehyde, hydroxypyruvate, formate, acetate, oxalate, 3-hydroxypropionate, DL-glycerate, pyruvate, and phenylpyruvate, formaldehyde, acetaldehyde, glutaraldehyde, glyoxal, methylglyoxal, and phenylglyoxal. The enzyme does not catalyze NAD(P)+-dependent glycolate oxidation at pH 4.0 and 7.0. DL-lactate, L-malate, (S)-hydroxyisobutyrate, and (R)-hydroxyisobutyrate, D-serine, L-serine, D-threonine, and L-threonine are inert as substrates of the enzyme when examined at pH of 4.0, 6.0, and 9.0 | Acetobacter aceti | ? | - |
- |
|
additional information | the enzyme exhibits no activity against succinic semialdehyde, hydroxypyruvate, formate, acetate, oxalate, 3-hydroxypropionate, DL-glycerate, pyruvate, and phenylpyruvate, formaldehyde, acetaldehyde, glutaraldehyde, glyoxal, methylglyoxal, and phenylglyoxal. The enzyme does not catalyze NAD(P)+-dependent glycolate oxidation at pH 4.0 and 7.0. DL-lactate, L-malate, (S)-hydroxyisobutyrate, and (R)-hydroxyisobutyrate, D-serine, L-serine, D-threonine, and L-threonine are inert as substrates of the enzyme when examined at pH of 4.0, 6.0, and 9.0 | Acetobacter aceti JCM20276 | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 328000, calculated from amino acid sequence | Acetobacter aceti |
? | x * 330000, SDS-PAGE | Acetobacter aceti |
Synonyms | Comment | Organism |
---|---|---|
aac4036 | - |
Acetobacter aceti |
glyoxylate reductase | - |
Acetobacter aceti |
NAD(P)H-dependent GR | - |
Acetobacter aceti |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Acetobacter aceti |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 45 | the enzyme shows relatively high activity over a broad temperature range (30-45°C) | Acetobacter aceti |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
the enzyme retains 90% activity at up to 65°C for 10 min and inactivated rapidly when the temperature exceeds 70°C | Acetobacter aceti |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.8 | - |
glycolate | with NAD+ as cosubstrate, at pH 9.0 and 45°C | Acetobacter aceti | |
10 | - |
glycolate | with NADP+ as cosubstrate, at pH 9.0 and 45°C | Acetobacter aceti | |
530 | - |
glyoxylate | with NADH as cosubstrate, at pH 4.0 and 45°C | Acetobacter aceti | |
570 | - |
glyoxylate | with NADPH as cosubstrate, at pH 4.0 and 45°C | Acetobacter aceti |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Acetobacter aceti |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 8 | approximately 50% of the activity is retained at a pH of 3.5 and 8.0, respectively | Acetobacter aceti |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 11 | more than 80% of the activity is retained over the pH range of 4.0-11.0 for 1 h on ice | Acetobacter aceti |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Acetobacter aceti | |
NADPH | - |
Acetobacter aceti |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.048 | - |
glycolate | with NAD+ as cosubstrate, at pH 9.0 and 45°C | Acetobacter aceti | |
0.1 | - |
glycolate | with NADP+ as cosubstrate, at pH 9.0 and 45°C | Acetobacter aceti | |
910 | - |
glyoxylate | with NADH as cosubstrate, at pH 4.0 and 45°C | Acetobacter aceti | |
1500 | - |
glyoxylate | with NADPH as cosubstrate, at pH 4.0 and 45°C | Acetobacter aceti |