Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | without EDTA the rates are about 60% of the maximal rate | Oryctolagus cuniculus | |
glycine buffer | 0.9 M, slight activation | Oryctolagus cuniculus |
Crystallization (Comment) | Organism |
---|---|
- |
Oryctolagus cuniculus |
General Stability | Organism |
---|---|
charcoal inactivates rabbit enzyme, restorable by thiamic acid | Oryctolagus cuniculus |
EDTA stabilizes | Oryctolagus cuniculus |
even cold dilute solutions in 0.2 M ammonium sulfate at pH 5.8 are stable for weeks | Oryctolagus cuniculus |
redistilled water prevents denaturation in absence of salts | Oryctolagus cuniculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,3-Dimercaptopropanol | competitive inhibitor to glycerophosphate | Oryctolagus cuniculus | |
iodoacetate | - |
Homo sapiens | |
iodoacetate | 0.1 mM 23% inhibition | Oryctolagus cuniculus | |
iodoacetate | - |
Rattus norvegicus | |
additional information | coenzyme analogs: acetylpyridine-NAD+, deaminoacetylpyridine-NAD+, pyridinealdehyde-NAD+, deaminopyridinealdehyde-NAD+, potent inhibitors | Oryctolagus cuniculus | |
N-ethylmaleimide | 0.1 mM complete inhibition | Oryctolagus cuniculus | |
N-ethylmaleimide | - |
Rattus norvegicus | |
p-mercuribenzoate | 0.1 mM complete inhibition | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
alpha-glycerophosphate | - |
Oryctolagus cuniculus | |
0.38 | - |
NAD+ | - |
Oryctolagus cuniculus | |
0.46 | - |
dihydroxyacetone phosphate | - |
Oryctolagus cuniculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Rattus norvegicus | 5737 | - |
cytoplasm | - |
Oryctolagus cuniculus | 5737 | - |
cytoplasm | - |
Locusta migratoria | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78000 | - |
- |
Rattus norvegicus |
78000 | - |
calculated from partial specific volume, sedimentation and diffusion data | Oryctolagus cuniculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sn-glycerol 3-phosphate + NAD+ | Homo sapiens | equilibrium far to the side of alpha-glycerophosphate at neutral pH | dihydroxyacetone phosphate + NADH + H+ | - |
? | |
sn-glycerol 3-phosphate + NAD+ | Rattus norvegicus | equilibrium far to the side of alpha-glycerophosphate at neutral pH | dihydroxyacetone phosphate + NADH + H+ | - |
? | |
sn-glycerol 3-phosphate + NAD+ | Oryctolagus cuniculus | equilibrium far to the side of alpha-glycerophosphate at neutral pH | dihydroxyacetone phosphate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Homo sapiens | - |
- |
- |
Locusta migratoria | - |
- |
- |
Mus musculus | - |
- |
- |
Oryctolagus cuniculus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH + H+ | one active site per enzyme molecule | Homo sapiens | |
sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH + H+ | one active site per enzyme molecule | Rattus norvegicus | |
sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH + H+ | one active site per enzyme molecule | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
breast cancer cell | - |
Homo sapiens | - |
kidney | - |
Homo sapiens | - |
kidney | - |
Rattus norvegicus | - |
leukocyte | also other blood components | Homo sapiens | - |
leukocyte | also other blood components | Rattus norvegicus | - |
liver | - |
Homo sapiens | - |
liver | - |
Rattus norvegicus | - |
liver | - |
Oryctolagus cuniculus | - |
muscle | - |
Mus musculus | - |
muscle | - |
Rattus norvegicus | - |
muscle | - |
Oryctolagus cuniculus | - |
muscle | bovine smooth muscles | Bos taurus | - |
sarcoma cell | - |
Homo sapiens | - |
sarcoma cell | - |
Oryctolagus cuniculus | - |
sarcoma cell | Crocker sarcoma | Mus musculus | - |
Storage Stability | Organism |
---|---|
4°C, dilute solution, pH 5.8, stable for weeks | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | - |
Oryctolagus cuniculus | ? | - |
? | |
sn-glycerol 3-phosphate + NAD+ | - |
Homo sapiens | dihydroxyacetone phosphate + NADH + H+ | - |
? | |
sn-glycerol 3-phosphate + NAD+ | - |
Rattus norvegicus | dihydroxyacetone phosphate + NADH + H+ | - |
? | |
sn-glycerol 3-phosphate + NAD+ | - |
Oryctolagus cuniculus | dihydroxyacetone phosphate + NADH + H+ | - |
? | |
sn-glycerol 3-phosphate + NAD+ | equilibrium far to the side of alpha-glycerophosphate at neutral pH | Homo sapiens | dihydroxyacetone phosphate + NADH + H+ | - |
? | |
sn-glycerol 3-phosphate + NAD+ | equilibrium far to the side of alpha-glycerophosphate at neutral pH | Rattus norvegicus | dihydroxyacetone phosphate + NADH + H+ | - |
? | |
sn-glycerol 3-phosphate + NAD+ | equilibrium far to the side of alpha-glycerophosphate at neutral pH | Oryctolagus cuniculus | dihydroxyacetone phosphate + NADH + H+ | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
stable for at least 30 min | Oryctolagus cuniculus |
55 | - |
t1/2: 1 min | Oryctolagus cuniculus |
60 | - |
- |
Mus musculus |
60 | - |
- |
Homo sapiens |
60 | - |
- |
Rattus norvegicus |
60 | - |
- |
Bos taurus |
60 | - |
complete inactivation after 1 min | Oryctolagus cuniculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
345 | - |
dihydroxyacetone phosphate | reduction of synthetic dihydroxyacetone phosphate, 20°C, pH 7, rabbit muscle | Oryctolagus cuniculus | |
390 | - |
dihydroxyacetone phosphate | reduction of synthetic dihydroxyacetone phosphate, 20°C, pH 7, rabbit muscle, under conditions where competitive inhibition by fructose 1,6-diphosphate are not excluded | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
reduction of dihydroxyacetone phosphate, in 0.2 M phosphate buffer | Oryctolagus cuniculus |
10.2 | - |
oxidation of glycerol 3-phosphate, in 0.03 glycine buffer | Oryctolagus cuniculus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.7 | 8.5 | around this range, most stable | Oryctolagus cuniculus |
7 | - |
less stable | Oryctolagus cuniculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | - |
Homo sapiens | |
additional information | deamino-NAD+ 60.3% as effective as NAD+ | Oryctolagus cuniculus | |
NAD+ | - |
Mus musculus | |
NAD+ | - |
Homo sapiens | |
NAD+ | - |
Rattus norvegicus | |
NAD+ | - |
Bos taurus | |
NAD+ | - |
Oryctolagus cuniculus | |
NADH | - |
Mus musculus | |
NADH | - |
Bos taurus | |
NADH | 1 mol per mol enzyme | Homo sapiens | |
NADH | 1 mol per mol enzyme | Rattus norvegicus | |
NADH | 1 mol per mol enzyme | Oryctolagus cuniculus |