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Literature summary for 1.1.1.80 extracted from

  • Sutak, R.; Hrdy, I.; Dolezal, P.; Cabala, R.; Sedinova, M.; Lewin, J.; Harant, K.; Mueller, M.; Tachezy, J.
    Secondary alcohol dehydrogenase catalyzes the reduction of exogenous acetone to 2-propanol in Trichomonas vaginalis (2012), FEBS J., 279, 2768-2780.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2,2'-dipyridyl 5 mM, 80% residual activity Trichomonas vaginalis
4-hydroxymercuribenzoic acid 0.1 mM, 49% residual activity Trichomonas vaginalis
4-Methylpyrazole 5 mM, 68% residual activity Trichomonas vaginalis
iodacetamide 50 mM, 49% residual activity Trichomonas vaginalis
additional information not inhibitory: EDTA up to 5 mM Trichomonas vaginalis
pyrazole 5 mM, 74% residual activity Trichomonas vaginalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000061
-
Butanone 37°C, pH 7.0 Trichomonas vaginalis
0.000093
-
acetone 37°C, pH 7.0 Trichomonas vaginalis
0.000256
-
pentan-3-one 37°C, pH 7.0 Trichomonas vaginalis
0.00028
-
acetaldehyde 37°C, pH 7.0 Trichomonas vaginalis
0.00056
-
propan-2-ol 37°C, pH 9.0 Trichomonas vaginalis
0.00078
-
butan-2-ol 37°C, pH 9.0 Trichomonas vaginalis
0.0015
-
pentan-3-ol 37°C, pH 9.0 Trichomonas vaginalis
0.0052
-
NADP+ pH 7.0, 37°C, cosubstrate acetone Trichomonas vaginalis
0.0056
-
pentan-2-ol 37°C, pH 9.0 Trichomonas vaginalis
0.0275
-
propan-1-ol 37°C, pH 9.0 Trichomonas vaginalis
0.1
-
ethanol 37°C, pH 9.0 Trichomonas vaginalis
0.51
-
NADPH pH 9.0, 37°C, cosubstrate propan-2-ol Trichomonas vaginalis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Trichomonas vaginalis 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39100
-
2 * 43000, SDS-PAGE, 2 * 39100, calculated Trichomonas vaginalis
43000
-
2 * 43000, SDS-PAGE, 2 * 39100, calculated Trichomonas vaginalis
81000
-
gel filtration Trichomonas vaginalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
butan-2-ol + NADP+ Trichomonas vaginalis preferred direction of the reaction is reduction rather than oxidation butanone + NADPH + H+
-
r
propan-2-ol + NADP+ Trichomonas vaginalis preferred direction of the reaction is reduction rather than oxidation acetone + NADPH + H+
-
r

Organism

Organism UniProt Comment Textmining
Trichomonas vaginalis A2DJ40 putative
-

Purification (Commentary)

Purification (Comment) Organism
-
Trichomonas vaginalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + NADPH + H+
-
Trichomonas vaginalis ethanol + NADP+
-
r
acetone + NADPH + H+
-
Trichomonas vaginalis propan-2-ol + NADP+
-
r
butan-2-ol + NADP+ preferred direction of the reaction is reduction rather than oxidation Trichomonas vaginalis butanone + NADPH + H+
-
r
butanone + NADPH + H+
-
Trichomonas vaginalis butan-2-ol + NADP+
-
r
ethanol + NADP+
-
Trichomonas vaginalis acetaldehyde + NADPH + H+
-
r
pentan-2-ol + NADP+
-
Trichomonas vaginalis pentan-2-one + NADPH + H+
-
r
pentan-3-ol + NADP+
-
Trichomonas vaginalis pentan-3-one + NADPH + H+
-
r
pentan-3-one + NADPH + H+
-
Trichomonas vaginalis pentan-3-ol + NADP+
-
r
propan-1-ol + NADP+
-
Trichomonas vaginalis propanal + NADPH + H+
-
r
propan-2-ol + NADP+ preferred direction of the reaction is reduction rather than oxidation Trichomonas vaginalis acetone + NADPH + H+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 43000, SDS-PAGE, 2 * 39100, calculated Trichomonas vaginalis

Synonyms

Synonyms Comment Organism
S-ADH
-
Trichomonas vaginalis
TVAG_228780
-
Trichomonas vaginalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
reduction of acetone, citrate buffer Trichomonas vaginalis
6.5 7.5 reduction of acetone, Bis/Tris propane buffer Trichomonas vaginalis
8 8.5 oxidation of propan-2-ol, Bis/Tris propane buffer Trichomonas vaginalis
9.5
-
oxidation of propan-2-ol, glycine/HCl buffer Trichomonas vaginalis

Cofactor

Cofactor Comment Organism Structure
NADPH strictly specific for NADPH Trichomonas vaginalis

Expression

Organism Comment Expression
Trichomonas vaginalis iron limitation results in a 1.5fold increase in enzyme mRNA up