Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-dipyridyl | 5 mM, 80% residual activity | Trichomonas vaginalis | |
4-hydroxymercuribenzoic acid | 0.1 mM, 49% residual activity | Trichomonas vaginalis | |
4-Methylpyrazole | 5 mM, 68% residual activity | Trichomonas vaginalis | |
iodacetamide | 50 mM, 49% residual activity | Trichomonas vaginalis | |
additional information | not inhibitory: EDTA up to 5 mM | Trichomonas vaginalis | |
pyrazole | 5 mM, 74% residual activity | Trichomonas vaginalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000061 | - |
Butanone | 37°C, pH 7.0 | Trichomonas vaginalis | |
0.000093 | - |
acetone | 37°C, pH 7.0 | Trichomonas vaginalis | |
0.000256 | - |
pentan-3-one | 37°C, pH 7.0 | Trichomonas vaginalis | |
0.00028 | - |
acetaldehyde | 37°C, pH 7.0 | Trichomonas vaginalis | |
0.00056 | - |
propan-2-ol | 37°C, pH 9.0 | Trichomonas vaginalis | |
0.00078 | - |
butan-2-ol | 37°C, pH 9.0 | Trichomonas vaginalis | |
0.0015 | - |
pentan-3-ol | 37°C, pH 9.0 | Trichomonas vaginalis | |
0.0052 | - |
NADP+ | pH 7.0, 37°C, cosubstrate acetone | Trichomonas vaginalis | |
0.0056 | - |
pentan-2-ol | 37°C, pH 9.0 | Trichomonas vaginalis | |
0.0275 | - |
propan-1-ol | 37°C, pH 9.0 | Trichomonas vaginalis | |
0.1 | - |
ethanol | 37°C, pH 9.0 | Trichomonas vaginalis | |
0.51 | - |
NADPH | pH 9.0, 37°C, cosubstrate propan-2-ol | Trichomonas vaginalis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Trichomonas vaginalis | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39100 | - |
2 * 43000, SDS-PAGE, 2 * 39100, calculated | Trichomonas vaginalis |
43000 | - |
2 * 43000, SDS-PAGE, 2 * 39100, calculated | Trichomonas vaginalis |
81000 | - |
gel filtration | Trichomonas vaginalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
butan-2-ol + NADP+ | Trichomonas vaginalis | preferred direction of the reaction is reduction rather than oxidation | butanone + NADPH + H+ | - |
r | |
propan-2-ol + NADP+ | Trichomonas vaginalis | preferred direction of the reaction is reduction rather than oxidation | acetone + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichomonas vaginalis | A2DJ40 | putative | - |
Purification (Comment) | Organism |
---|---|
- |
Trichomonas vaginalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + NADPH + H+ | - |
Trichomonas vaginalis | ethanol + NADP+ | - |
r | |
acetone + NADPH + H+ | - |
Trichomonas vaginalis | propan-2-ol + NADP+ | - |
r | |
butan-2-ol + NADP+ | preferred direction of the reaction is reduction rather than oxidation | Trichomonas vaginalis | butanone + NADPH + H+ | - |
r | |
butanone + NADPH + H+ | - |
Trichomonas vaginalis | butan-2-ol + NADP+ | - |
r | |
ethanol + NADP+ | - |
Trichomonas vaginalis | acetaldehyde + NADPH + H+ | - |
r | |
pentan-2-ol + NADP+ | - |
Trichomonas vaginalis | pentan-2-one + NADPH + H+ | - |
r | |
pentan-3-ol + NADP+ | - |
Trichomonas vaginalis | pentan-3-one + NADPH + H+ | - |
r | |
pentan-3-one + NADPH + H+ | - |
Trichomonas vaginalis | pentan-3-ol + NADP+ | - |
r | |
propan-1-ol + NADP+ | - |
Trichomonas vaginalis | propanal + NADPH + H+ | - |
r | |
propan-2-ol + NADP+ | preferred direction of the reaction is reduction rather than oxidation | Trichomonas vaginalis | acetone + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 43000, SDS-PAGE, 2 * 39100, calculated | Trichomonas vaginalis |
Synonyms | Comment | Organism |
---|---|---|
S-ADH | - |
Trichomonas vaginalis |
TVAG_228780 | - |
Trichomonas vaginalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
reduction of acetone, citrate buffer | Trichomonas vaginalis |
6.5 | 7.5 | reduction of acetone, Bis/Tris propane buffer | Trichomonas vaginalis |
8 | 8.5 | oxidation of propan-2-ol, Bis/Tris propane buffer | Trichomonas vaginalis |
9.5 | - |
oxidation of propan-2-ol, glycine/HCl buffer | Trichomonas vaginalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | strictly specific for NADPH | Trichomonas vaginalis |
Organism | Comment | Expression |
---|---|---|
Trichomonas vaginalis | iron limitation results in a 1.5fold increase in enzyme mRNA | up |