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Literature summary for 1.1.1.81 extracted from

  • Chistoserdova, L.V.; Lidstrom, M.E.
    Purification and characterization of hydroxypyruvate reductase from the facultative methylotroph Methylobacterium extorquens AM1 (1991), J. Bacteriol., 173, 7228-7232.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
citrate 5 mM, 20% inhibition of NADH linked hydroxypyruvate reduction, 25% of NADPH linked reduction Methylorubrum extorquens AM1
D-glycerate 5 mM, 20% inhibition of NADH linked hydroxypyruvate reduction, 40% of NADPH linked reduction Methylorubrum extorquens AM1
glycolate 5 mM, 15% of NADPH linked reduction Methylorubrum extorquens AM1
glyoxylate 10 mM, 55% inhibition of NADH linked hydroxypyruvate reduction, 15% of NADPH linked reduction, 80% of NAD+ linked glycerate oxidation Methylorubrum extorquens AM1
Hydroxypyruvate 0.5 mM, 90% of NAD+ linked glycerate oxidation Methylorubrum extorquens AM1
NAD+ 4 mM, 10% inhibition of NADH linked hydroxypyruvate reduction, 60% of NADPH linked reduction Methylorubrum extorquens AM1
NADP+ 4 mM, 15% inhibition of NADH linked hydroxypyruvate reduction, 40% of NADPH linked reduction, 8% of NAD+ linked glycerate oxidation Methylorubrum extorquens AM1
oxaloacetate 2.5 mM, 10% inhibition of NADH linked hydroxypyruvate reduction, 20% of NADPH linked reduction Methylorubrum extorquens AM1
pyruvate 10 mM, 40% inhibition of NADH linked hydroxypyruvate reduction Methylorubrum extorquens AM1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
NADH
-
Methylorubrum extorquens AM1
0.06
-
NADPH
-
Methylorubrum extorquens AM1
0.1
-
Hydroxypyruvate cofactor NADH Methylorubrum extorquens AM1
1.25
-
Hydroxypyruvate cofactor NADPH Methylorubrum extorquens AM1
2.6
-
glycerate
-
Methylorubrum extorquens AM1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
2 * 37000, SDS-PAGE Methylorubrum extorquens AM1
71000
-
gradient PAGE Methylorubrum extorquens AM1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hydroxypyruvate + NAD(P)H Methylorubrum extorquens AM1 cofactor NADPH, 80% of activity with NADH, rate of oxidation reaction: 1.5% of reduction reaction only with cofactor NADH D-glycerate + NAD(P)+ + H+
-
r

Organism

Organism UniProt Comment Textmining
Methylorubrum extorquens AM1
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
675
-
-
Methylorubrum extorquens AM1

Storage Stability

Storage Stability Organism
4°C, 50 mM Tris-HCl, pH 7.5, 10% glycerol, 1 mM dithiothreitol, 2 months, 10% loss of activity Methylorubrum extorquens AM1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glycerate + NAD+
-
Methylorubrum extorquens AM1 3-hydroxypyruvate + NADH + H+
-
r
glyoxylate + NAD(P)H cofactor NADH, 15% of activity with hydroxypyruvate, cofactor NADPH, 1.5% of activity with hydroxypyruvate Methylorubrum extorquens AM1 glycolate + NAD(P)+
-
?
hydroxypyruvate + NAD(P)H
-
Methylorubrum extorquens AM1 D-glycerate + NAD(P)+ + H+
-
?
hydroxypyruvate + NAD(P)H cofactor NADPH, 80% of activity with NADH, rate of oxidation reaction: 1.5% of reduction reaction only with cofactor NADH Methylorubrum extorquens AM1 D-glycerate + NAD(P)+ + H+
-
r
hydroxypyruvate + NADH + H+
-
Methylorubrum extorquens AM1 D-glycerate + NAD+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 37000, SDS-PAGE Methylorubrum extorquens AM1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
broad optimum between pH 4.0 and 6.5 for hydroxypyruvate reduction Methylorubrum extorquens AM1
9 11 glycerate oxidation Methylorubrum extorquens AM1