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Literature summary for 1.1.1.82 extracted from

  • Kagawa, T.; Bruno, P.L.
    NADP-malate dehydrogenase from leaves of Zea mays: purification and physical, chemical, and kinetic properties (1988), Arch. Biochem. Biophys., 260, 674-695.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol activates Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
NADPH
-
Zea mays
0.056
-
oxaloacetate cosubstrate NADPH Zea mays
0.061
-
oxaloacetate cosubstrate NADH Zea mays
0.073
-
NADP+ cosubstrate malate Zea mays
0.8
-
NAD+ cosubstrate malate Zea mays
0.83
-
NADH cosubstrate oxaloacetate Zea mays
29
-
(S)-malate cosubstrate NAD+ Zea mays
32
-
(S)-malate cosubstrate NADP+ Zea mays

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
2 * 43000, SDS-PAGE Zea mays
88000
-
gradient pore-PAGE Zea mays
91000
-
gel filtration Zea mays

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
oxaloacetate + NADPH Zea mays key enzyme of the photosynthetic pathway of C4 plants (S)-malate + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Zea mays
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Zea mays
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
945
-
-
Zea mays

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
Zea mays oxaloacetate + NADH + H+
-
r
(S)-malate + NADP+
-
Zea mays oxaloacetate + NADPH + H+
-
r
oxaloacetate + NADH
-
Zea mays (S)-malate + NAD+
-
r
oxaloacetate + NADPH
-
Zea mays (S)-malate + NADP+
-
r
oxaloacetate + NADPH key enzyme of the photosynthetic pathway of C4 plants Zea mays (S)-malate + NADP+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 43000, SDS-PAGE Zea mays

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
NADPH-dependent reduction of oxaloacetate Zea mays